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A defensin from clam Venerupis philippinarum: Molecular characterization, localization, antibacterial activity, and mechanism of action
Zhang, LB; Yang, DL; Wang, Q; Yuan, ZY; Wu, HF; Pei, D; Cong, M; Li, F; Ji, CL; Zhao, JM; Wu, HF (reprint author), YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China. hfwu@yic.ac.cn; jmzhao@yic.ac.cn
2015-07-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume51Issue:1Pages:29-38
Contribution Rank[Zhang, Linbao; Yang, Dinglong; Wang, Qing; Wu, Huifeng; Pei, Dong; Cong, Ming; Li, Fei; Ji, Chenglong; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai, Peoples R China; [Zhang, Linbao; Yang, Dinglong; Wang, Qing; Wu, Huifeng; Pei, Dong; Cong, Ming; Li, Fei; Ji, Chenglong; Zhao, Jianmin] YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Zhang, Linbao] Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China; [Yang, Dinglong] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Yuan, Zeyi] Natl Marine Data & Informat Serv, Tianjin 300171, Peoples R China
AbstractAntimicrobial peptides (AMPs) are important mediators of the primary host defense system against microbial invasion. In the present study, we cloned and characterized a member of the invertebrate defensin from the clam Venerupis philippinarum, designated VpDef. Amino acid sequence analysis showed that VpDef was similar to defensins from marine mollusks and ticks. In non-stimulated clams, RT-PCR and immunohistochemical analysis revealed that both VpDef mRNA and the encoding peptide were constitutively expressed in hemocytes and mantles, as well as in other major tissues. VpDef transcripts were significantly induced in hemocytes at different time intervals post Vibrio anguillarum infection. The recombinant VpDef (rVpDef) showed the highest activity against Gram-positive bacteria Micrococcus luteus and less effective to Gram-negative bacteria. In addition, incubation of rVpDef with M. luteus at 1 x and 3 x MIC could induce an obvious decrease of the membrane potential and notable changes of membrane permeability in a dose-dependent manner. Membrane integrity and bacterial viability analysis also revealed that rVpDef increased the membrane permeability of M. luteus and then resulted in cell death at 2 x and 10 x MIC. Overall, these results suggest that VpDef has an important function in host defense against invasive pathogens, probably killing microbes by inducing membrane lesions. (C) 2015 Elsevier Ltd. All rights reserved.
KeywordVenerupis Philippinarum Defensin Molecular Characterization Tissue Distribution Antimicrobial Activity Membrane Lesions
Department中科院海岸带环境过程与生态修复重点实验室
DOI10.1016/j.dci.2015.02.009
Indexed BySCI
WOS KeywordOYSTER CRASSOSTREA-GIGAS ; RICH ANTIMICROBIAL PEPTIDES ; PRECURSOR LIPID II ; MARINE-INVERTEBRATES ; ESCHERICHIA-COLI ; RUDITAPES-PHILIPPINARUM ; MICROCOCCUS-LUTEUS ; ARTHROPOD DEFENSIN ; INNATE IMMUNITY ; MEMBRANE
SubtypeArticle
Language英语
WOS Research AreaImmunology ; Zoology
WOS IDWOS:000357906800004
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Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/9274
Collection中科院海岸带环境过程与生态修复重点实验室
Corresponding AuthorWu, HF (reprint author), YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China. hfwu@yic.ac.cn; jmzhao@yic.ac.cn
Recommended Citation
GB/T 7714
Zhang, LB,Yang, DL,Wang, Q,et al. A defensin from clam Venerupis philippinarum: Molecular characterization, localization, antibacterial activity, and mechanism of action[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2015,51(1):29-38.
APA Zhang, LB.,Yang, DL.,Wang, Q.,Yuan, ZY.,Wu, HF.,...&jmzhao@yic.ac.cn.(2015).A defensin from clam Venerupis philippinarum: Molecular characterization, localization, antibacterial activity, and mechanism of action.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,51(1),29-38.
MLA Zhang, LB,et al."A defensin from clam Venerupis philippinarum: Molecular characterization, localization, antibacterial activity, and mechanism of action".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 51.1(2015):29-38.
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