YIC-IR中国科学院烟台海岸带研究所http://ir.yic.ac.cn:802024-03-19T12:25:58Z2024-03-19T12:25:58ZHow can Phycobilisome, the unique light harvesting system in certain algae working highly efficiently: The connection in between structures and functionsLiu, RunzeZhen, Zhang-HeLi, WenjunGe, BaoshengQin, Songhttp://ir.yic.ac.cn:80/handle/133337/343812024-02-23T10:54:06Z2024-02-23T10:54:06Z题名: How can Phycobilisome, the unique light harvesting system in certain algae working highly efficiently: The connection in between structures and functions
作者: Liu, Runze; Zhen, Zhang-He; Li, Wenjun; Ge, Baosheng; Qin, Song
摘要: Algae, which are ubiquitous in ecosystems, have evolved a variety of light-harvesting complexes to better adapt to diverse habitats. Phycobilisomes/phycobiliproteins, unique to cyanobacteria, red algae, and certain cryptomonads, compensate for the lack of chlorophyll absorption, allowing algae to capture and efficiently transfer light energy in aquatic environments. With the advancement of microscopy and spectroscopy, the structure and energy transfer processes of increasingly complex phycobilisomes have been elucidated, providing us with a vivid portrait of the dynamic adaptation of their structures to the light environment in which algae thrive: 1) Cyanobacteria living on the surface of the water use short, small phycobilisomes to absorb red-orange light and reduce the damage from blue-violet light via multiple methods; 2) Large red algae inhabiting the depths of the ocean have evolved long and dense phycobilisomes containing phycoerythrin to capture the feeble blue-green light; 3) In far-red light environments such as caves, algae use special allophycocyanin cores to optimally utilize the far-red light; 4) When the environment shifts, algae can adjust the length, composition and density of their rods to better adapt; 5) By carefully designing the position of the pigments, phycobilisomes can transfer light energy to the reaction center with nearly 100% efficiency via three energy transfer processes.2024-02-23T10:54:06ZTranscriptomic Responses of a Lightly Calcified Echinoderm to Experimental Seawater Acidification and Warming during Early DevelopmentZhao, YeSong, MingshanYu, ZhenglinPang, LeiZhang, LibinKarakassis, IoannisDimitriou, Panagiotis D.Yuan, Xiutanghttp://ir.yic.ac.cn:80/handle/133337/343782024-02-23T10:54:05Z2024-02-23T10:54:05Z题名: Transcriptomic Responses of a Lightly Calcified Echinoderm to Experimental Seawater Acidification and Warming during Early Development
作者: Zhao, Ye; Song, Mingshan; Yu, Zhenglin; Pang, Lei; Zhang, Libin; Karakassis, Ioannis; Dimitriou, Panagiotis D.; Yuan, Xiutang
摘要: Ocean acidification (OA) and ocean warming (OW) are potential obstacles to the survival and growth of marine organisms, particularly those that rely on calcification. This study investigated the single and joint effects of OA and OW on sea cucumber Apostichopus japonicus larvae raised under combinations of two temperatures (19 degrees C or 22 degrees C) and two pCO(2) levels (400 or 1000 mu atm) that reflect the current and end-of-21st-century projected ocean scenarios. The investigation focused on assessing larval development and identifying differences in gene expression patterns at four crucial embryo-larval stages (blastula, gastrula, auricularia, and doliolaria) of sea cucumbers, using RNA-seq. Results showed the detrimental effect of OA on the early development and body growth of A. japonicus larvae and a reduction in the expression of genes associated with biomineralization, skeletogenesis, and ion homeostasis. This effect was particularly pronounced during the doliolaria stage, indicating the presence of bottlenecks in larval development at this transition phase between the larval and megalopa stages in response to OA. OW accelerated the larval development across four stages of A. japonicus, especially at the blastula and doliolaria stages, but resulted in a widespread upregulation of genes related to heat shock proteins, antioxidant defense, and immune response. Significantly, the negative effects of elevated pCO(2) on the developmental process of larvae appeared to be mitigated when accompanied by increased temperatures at the expense of reduced immune resilience and increased system fragility. These findings suggest that alterations in gene expression within the larvae of A. japonicus provide a mechanism to adapt to stressors arising from a rapidly changing oceanic environment.2024-02-23T10:54:05ZGenome-Wide Identification and Characterization of the Hsp70/110 Genes in Two Polychaetes, <i>Capitella teleta</i> and <i>Owenia fusiformis</i>Gao, J.Jiang, X.Liu, L.Shi, Y.Sun, C.Gao, C.Sui, F.Wang, W.Song, X.http://ir.yic.ac.cn:80/handle/133337/343752024-02-23T10:54:03Z2024-02-23T10:54:03Z题名: Genome-Wide Identification and Characterization of the Hsp70/110 Genes in Two Polychaetes, <i>Capitella teleta</i> and <i>Owenia fusiformis</i>
作者: Gao, J.; Jiang, X.; Liu, L.; Shi, Y.; Sun, C.; Gao, C.; Sui, F.; Wang, W.; Song, X.
摘要: Hsp70/110s are a subfamily of heat shock proteins that play crucial roles against different types of environmental stressors and pathogenic organisms. To understand the evolution and divergence patterns of the Hsp70/110 gene family in polychaetes, genome-wide data from Capitella teleta and Owenia fusiformis were systematically analyzed based on bioinformatics methods. A total of 32 Hsp70/110 genes were identified in C. teleta, while only 11 Hsp70/110 genes were found in O. fusiformis. The phylogenetic tree supported that these genes were divided into 9 subfamilies. Selective pressure analysis showed that the Hsp70/110 gene family in polychaetes has evolved under purifying selection. Furthermore, diverse expression profiles were displayed for different Hsp70/110 genes in the two polychaetes. These results provide a theoretical basis for further studies of the molecular evolutionary patterns and function of the Hsp70/110 gene family in polychaetes.2024-02-23T10:54:03ZIdentifying the key factors influencing spatial and temporal variations of regional coastal fishing activitiesChen, RenliWang, YueqiWu, XiaoqingGao, Zhiqianghttp://ir.yic.ac.cn:80/handle/133337/343722024-02-23T10:54:01Z2024-02-23T10:54:01Z题名: Identifying the key factors influencing spatial and temporal variations of regional coastal fishing activities
作者: Chen, Renli; Wang, Yueqi; Wu, Xiaoqing; Gao, Zhiqiang
摘要: Fishing is one of the most pervasive human activities for harvesting natural resources. Accurate understanding of the dynamic variations of regional fishing activities and their influence mechanisms is crucial for marine conservation and fisheries management. Previous studies have described the spatio-temporal distribution of fishing operations. However, there is still a need for a comprehensive interpretation of why and how these variations occur, especially in regional coastal waters. In this study, taking the Bohai Sea and its adjacent waters as an example, the correlation between regional fishing activities and multiple factors, including the marine environment, geographical position, socio-economic conditions, and marine management policies, was investigated from the perspective of human geography. Automatic Identification System (AIS) data for fishing vessels were used, combining remote sensing, geographic information system (GIS), and statistical models. The results indicated that the spatio-temporal variations of regional coastal fishing activities were driven by complex interactions among these factors. Overall, the average explanatory power of socio-economic and geographical position factors on fishing activities was higher than that of marine environmental factors. The importance of the marine environment and geographical position for fishing activities varied throughout different seasons. The sensitivity of fishing activities to geographical position was relatively high, with the explained deviance exceeding 62% of the total explained deviance. Intensive fishing was primarily observed within a particular range of the sea surface temperature frontal gradient (0.20-0.60 degrees C/km) or sea surface chlorophyll-a frontal gradient (1.06-1.25). The spatial distribution of fishing activities is effectively constrained by implemented marine management policies. This study sheds new light on the key factors influencing regional fishing activities in coastal waters and highlights the complexity of their interaction effects. These findings can provide a theoretical basis for the restoration of fishery resources and marine fishing management.2024-02-23T10:54:01ZIdentification, Evolution and Expression Analysis of Dmrt Genes in PolychaetesJi, Y. L.Shen, X. H.Tian, S. J.Liu, H.Cao, T. G.Wang, Q. C.Wang, Y.http://ir.yic.ac.cn:80/handle/133337/343692024-02-23T10:53:59Z2024-02-23T10:53:59Z题名: Identification, Evolution and Expression Analysis of Dmrt Genes in Polychaetes
作者: Ji, Y. L.; Shen, X. H.; Tian, S. J.; Liu, H.; Cao, T. G.; Wang, Q. C.; Wang, Y.
摘要: The doublesex-mab3-related transcription factor (Dmrt) gene family is a class of crucial transcription factors characterized by one or several DM domains. Dmrt family genes play important roles in sex determination/differentiation. Polychaete annelids are critically important in most marine ecosystems. However, to date, the genome-wide characterization and analysis of Dmrt genes in polychaetes have not been investigated. In this study, the identification and analysis of Dmrt genes were performed in three representative polychaetes, including Capitella teleta, Dimorphilus gyrociliatus, and Owenia fusiformis. A total of 12 Dmrt genes have been found, and the number of Dmrt genes in different polychaetes ranges from 3 to 5. The phylogenetic tree showed that all identified Dmrt genes were classified into 4 classes: Dmrt2/11E, Dmrt3/93B, Dmrt4/5/99B, and DSX. Furthermore, the selection pressure analysis suggested that all the Dmrt genes underwent strong purifying selection pressure. The spatiotemporal expression profile in O. fusiformis suggested that Dmrt genes have diverse functions, and the Dmrt2/11E-like gene may play an important role in the sex determination/differentiation of polychaetes. In general, this study provides a molecular basis for polychaete Dmrt genes and may serve as a reference for in-depth phylogenomics.2024-02-23T10:53:59ZCoastline Monitoring and Prediction Based on Long-Term Remote Sensing Data-A Case Study of the Eastern Coast of Laizhou Bay, ChinaMu, KeTang, ChengTosi, LuigiLi, YanfangZheng, XiangyangDonnici, SandraSun, JixiangLiu, JunGao, Xueluhttp://ir.yic.ac.cn:80/handle/133337/343662024-02-23T10:49:10Z2024-02-23T10:49:10Z题名: Coastline Monitoring and Prediction Based on Long-Term Remote Sensing Data-A Case Study of the Eastern Coast of Laizhou Bay, China
作者: Mu, Ke; Tang, Cheng; Tosi, Luigi; Li, Yanfang; Zheng, Xiangyang; Donnici, Sandra; Sun, Jixiang; Liu, Jun; Gao, Xuelu
摘要: Monitoring shoreline movements is essential for understanding the impact of anthropogenic activities and climate change on the coastal zone dynamics. The use of remote sensing allows for large-scale spatial and temporal studies to better comprehend current trends. This study used Landsat 5 (TM), Landsat 8 (OLI), and Sentinel-2 (MSI) remote sensing images, together with the Otsu algorithm, marching squares algorithm, and tidal correction algorithm, to extract and correct the coastline positions of the east coast of Laizhou Bay in China from 1984 to 2022. The results indicate that 89.63% of the extracted shoreline segments have an error less than 30 m compared to the manually drawn coastline. The total length of the coastline increased from 166.90 km to 364.20 km, throughout the observation period, with a length change intensity (LCI) of 3.11% due to the development of coastal protection and engineering structures for human activities. The anthropization led to a decrease in the natural coastline from 83.33% to 13.89% and a continuous increase in the diversity and human use of the coastline. In particular, the index of coastline diversity (ICTD) and the index of coastline utilization degree (ICUD) increased from 0.39 to 0.79, and from 153.30 to 390.37, respectively. Over 70% of the sandy beaches experienced erosional processes. The shoreline erosion calculated using the end point rate (EPR) and the linear regression rate (LRR) is 79.54% and 85.58%, respectively. The fractal dimension of the coastline shows an increasing trend and is positively correlated with human activities. Coastline changes are primarily attributed to interventions such as land reclamation, aquaculture development, and port construction resulting in the creation of 10,000.20 hectares of new coastal areas. Finally, the use of Kalman filtering for the first time made it possible to predict that approximately 84.58% of the sandy coastline will be eroded to varying degrees by 2032. The research results can provide valuable reference for the scientific planning and rational utilization of resources on the eastern coast of Laizhou Bay.2024-02-23T10:49:10ZInteractive effects of multiple antibiotic residues and ocean acidification on physiology and metabolome of the bay scallops Argopecten irradians irradiansZhang, TianyuWang, XinZhang, QianqianYang, DinglongZhang, XiaoliLiu, HuiDong, ZhijunZhao, Jianminhttp://ir.yic.ac.cn:80/handle/133337/343632024-02-23T10:49:08Z2024-02-23T10:49:08Z题名: Interactive effects of multiple antibiotic residues and ocean acidification on physiology and metabolome of the bay scallops Argopecten irradians irradians
作者: Zhang, Tianyu; Wang, Xin; Zhang, Qianqian; Yang, Dinglong; Zhang, Xiaoli; Liu, Hui; Dong, Zhijun; Zhao, Jianmin
摘要: Coastal areas are confronted with compounding threats arising from both climatic and non-climatic stressors. Antibiotic pollution and ocean acidification are two prevalently concurrent environmental stressors. Yet their interactive effects on marine biota have not been investigated adequately and the compound hazard remain obscure. In this study, bay scallops Argopecten irradians irradians were exposed to multiple antibiotics (sulfamethoxazole, tetracycline, oxytetracycline, norfloxacin, and erythromycin, each at a concentration of 1 mu g/L) combined with/without acidic seawater (pH 7.6) for 35 days. The single and interactive effects of the two stressors on A. irradians irradians were determined from multidimensional bio-responses, including energetic physiological traits as well as the molecular underpinning (metabolome and expressions of key genes). Results showed that multiple antibiotics predominantly enhanced the process of DNA repair and replication via disturbing the purine metabolism pathway. This alternation is perhaps to cope with the DNA damage induced by oxidative stress. Ocean acidification mainly disrupted energy metabolism and ammonia metabolism of the scallops, as evidenced by the increased ammonia excretion rate, the decreased O:N ratio, and perturbations in amino acid metabolism pathways. Moreover, the antagonistic effects of multiple antibiotics and ocean acidification caused alternations in the relative abundance of neurotransmitter and gene expression of neurotransmitter receptors, which may lead to neurological disorders in scallops. Overall, the revealed alternations in physiological traits, metabolites and gene expressions provide insightful information for the health status of bivalves in a natural environmental condition under the climate change scenarios.2024-02-23T10:49:08ZLong-Term Fertilization Contributes to Carbon Saturation in Neutral-To-Alkaline Soils but not in Acidic SoilsZhou, ShiweiLv, YanchaoSong, ZhizhongBi, XiaoliMeng, Linghttp://ir.yic.ac.cn:80/handle/133337/343602024-02-23T10:49:07Z2024-02-23T10:49:07Z题名: Long-Term Fertilization Contributes to Carbon Saturation in Neutral-To-Alkaline Soils but not in Acidic Soils
作者: Zhou, Shiwei; Lv, Yanchao; Song, Zhizhong; Bi, Xiaoli; Meng, Ling
摘要: The study investigated the adsorption of dissolved organic carbon (DOC) on Chinese soils from four long-term experiments involving five treatments: (1) no fertilization (control), (2-4) inorganic nitrogen (N), phosphorus (P), and potassium (K) fertilization (N, NP, and NPK), (5) NPK plus manure (NPKM). The results showed that DOC adsorption followed a modified Langmuir isotherm model effectively (R-2 = 0.912 similar to 0.991). The maximum adsorption capacity (Q(max)) (i.e. saturation deficits) generally increased with increasing soil organic carbon (SOC) due to fertilization in Luvic Phaeozem, Haplic Gypsisols, and Eutric Cambisols. However, it decreased significantly in acidic soils (Haplic Acrisols) from 15.59 g/kg for control, to 13.96, 13.03, 9.30 and 8.02 g/kg for N, NP, NPK and NPKM, respectively. Moreover, long-term fertilization, particularly with organic fertilization (e.g. NPKM), resulted in an increase of carbon (C) saturation by 1.42 g/kg (5.52%) in Luvic Phaeozem, 9.27 g/kg (44.29%) in Haplic Gypsisols, and 3.75 g/kg (27.66%) in Eutric Cambisols; however, there was a slight decrease by 1.70 g/kg (-7.49%) in Haplic Acrisols. Canonical correlation analysis (CCA) revealed that clay content and soil available iron (AFe) might be the crucial factors controlling C deficits, and subsequently, C saturation, along with current SOC. In conclusion, long-term fertilization contributed to C saturation in neutral-to-alkaline soils, but not in acidic soils. Thus, further attention should be paid to the long-term effects of fertilization on C saturation.2024-02-23T10:49:07ZElectrochemical Sensing System for Rapid <i>In Situ</i> Monitoring of Oceanic <i>p</i>CO<sub>2</sub>Liao, ZhiboJiang, TianjiaQin, Weihttp://ir.yic.ac.cn:80/handle/133337/343572024-02-23T10:49:05Z2024-02-23T10:49:05Z题名: Electrochemical Sensing System for Rapid <i>In Situ</i> Monitoring of Oceanic <i>p</i>CO<sub>2</sub>
作者: Liao, Zhibo; Jiang, Tianjia; Qin, Wei
摘要: Oceanic partial pressure of carbon dioxide (pCO(2)) monitoring is of great importance for fully understanding the inorganic carbon chemistry of seawater. However, the in situ determination of pCO(2) via electrochemical sensing is still a challenge as a result of the fluctuating salinities and temperatures in a marine environment. In this work, a marine pCO(2) monitoring system based on potentiometric sensing has been developed using real-time corrections for the salinity and temperature effects. The system consists of an electrochemical sensing unit based on an ion-selective electrode for selective carbonate detection, probes for seawater parameters (i.e., pH, salinity, and temperature), and a control system for autonomous sample analysis, sensor calibration, and pCO(2) calculation. The proposed sensing device offers a response time of about 22 s (t(95%)), a detection accuracy of <2 mu atm (mean deviation compared to the used standard CO2 gases), and a stability of 1.43% (mean relative standard deviation for the repeated measurements of the Tris-HCl buffer solutions equilibrated with different standard gases at pCO(2) levels of 450, 600, and 800 mu atm) for the real-time monitoring of pCO(2). The functioning of the marine pCO(2) monitoring system was validated via two different monitoring approaches (i.e., monitoring one site with a fixed duration and monitoring different sites in one sea region) in two different sea areas of China. The obtained results indicate the capability of the proposed pCO(2) monitoring system to autonomously operate for routine in situ monitoring in terms of sampling, detection, calibration, data storage, and transmission. The proposed electrochemical sensing system can be used for submersible and shipboard monitoring of oceanic pCO(2).2024-02-23T10:49:05ZNIR Light-Activated Mitochondrial RNA Cross-Linking Strategy for H<sub>2</sub>S Monitoring and Prolonged Colorectal Tumor ImagingLu, ZhihaoTan, JiangkunWu, YutingYou, JinmaoXie, XiunanZhang, ZhiyongLi, ZanChen, Lingxinhttp://ir.yic.ac.cn:80/handle/133337/343542024-02-23T10:49:04Z2024-02-23T10:49:04Z题名: NIR Light-Activated Mitochondrial RNA Cross-Linking Strategy for H<sub>2</sub>S Monitoring and Prolonged Colorectal Tumor Imaging
作者: Lu, Zhihao; Tan, Jiangkun; Wu, Yuting; You, Jinmao; Xie, Xiunan; Zhang, Zhiyong; Li, Zan; Chen, Lingxin
摘要: Molecular diffusion and leakage impede the long-term retention of probes/drugs and may cause potential adverse effects in theranostic fields. Spatiotemporally manipulating the organelle-immobilization behavior of probes/drugs for prolonged tumor retention is indispensable to achieving effective cancer diagnosis and therapy. Herein, we propose a rational strategy that could realize near-infrared light-activated ribonucleic acids (RNAs) cross-linking for prolonged tumor retention and simultaneously endogenous hydrogen sulfide (H2S) monitoring in colorectal tumors. Profiting from efficient singlet oxygen (O-1(2)) generation from Cy796 under 808 nm light irradiation, the O-1(2)-animated furan moiety in Cy796 could covalently cross-link with cytoplasmic RNAs via a cycloaddition reaction and realize organelle immobilization. Subsequently, specific thiolysis of Cy796 assisted with H2S resulted in homologous product Cy644 with reduced O-1(2) generation yields and enhanced absolute fluorescence quantum yields (from 7.42 to 27.70%) with blue-shifted absorption and emission, which avoided the molecular oxidation fluorescence quenching effect mediated by O-1(2) and validated fluorescence imaging. Furthermore, studies have demonstrated that our proposed strategy possessed adequate capacity for fluorescence imaging and endogenous H2S detection in HCT116 cells, particularly accumulated at the tumor sites, and retained long-term imaging with excellent biocompatibility. The turn-on fluorescence mode and turn-off (1)O(2 )generation efficiency in our strategy successfully realized a diminished fluorescence cross-talk and oxidation quenching effect. It is adequately envisioned that our proposed strategy for monitoring biomarkers and prolonged tumor retention will contribute tremendous dedication in the clinical, diagnostic, and therapeutic fields.2024-02-23T10:49:04Z