Institutional Repository of Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences (KLCEP)
| A defensin from clam Venerupis philippinarum: Molecular characterization, localization, antibacterial activity, and mechanism of action | |
Zhang, LB; Yang, DL ; Wang, Q; Yuan, ZY; Wu, HF; Pei, D; Cong, M; Li, F; Ji, CL; Zhao, JM; Wu, HF (reprint author), YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China. hfwu@yic.ac.cn; jmzhao@yic.ac.cn
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| 发表期刊 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
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| ISSN | 0145-305X |
| 2015-07-01 | |
| 卷号 | 51期号:1页码:29-38 |
| 关键词 | Venerupis Philippinarum Defensin Molecular Characterization Tissue Distribution Antimicrobial Activity Membrane Lesions |
| DOI | 10.1016/j.dci.2015.02.009 |
| 产权排序 | [Zhang, Linbao; Yang, Dinglong; Wang, Qing; Wu, Huifeng; Pei, Dong; Cong, Ming; Li, Fei; Ji, Chenglong; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai, Peoples R China; [Zhang, Linbao; Yang, Dinglong; Wang, Qing; Wu, Huifeng; Pei, Dong; Cong, Ming; Li, Fei; Ji, Chenglong; Zhao, Jianmin] YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Zhang, Linbao] Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China; [Yang, Dinglong] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Yuan, Zeyi] Natl Marine Data & Informat Serv, Tianjin 300171, Peoples R China |
| 作者部门 | 中科院海岸带环境过程与生态修复重点实验室 |
| 英文摘要 | Antimicrobial peptides (AMPs) are important mediators of the primary host defense system against microbial invasion. In the present study, we cloned and characterized a member of the invertebrate defensin from the clam Venerupis philippinarum, designated VpDef. Amino acid sequence analysis showed that VpDef was similar to defensins from marine mollusks and ticks. In non-stimulated clams, RT-PCR and immunohistochemical analysis revealed that both VpDef mRNA and the encoding peptide were constitutively expressed in hemocytes and mantles, as well as in other major tissues. VpDef transcripts were significantly induced in hemocytes at different time intervals post Vibrio anguillarum infection. The recombinant VpDef (rVpDef) showed the highest activity against Gram-positive bacteria Micrococcus luteus and less effective to Gram-negative bacteria. In addition, incubation of rVpDef with M. luteus at 1 x and 3 x MIC could induce an obvious decrease of the membrane potential and notable changes of membrane permeability in a dose-dependent manner. Membrane integrity and bacterial viability analysis also revealed that rVpDef increased the membrane permeability of M. luteus and then resulted in cell death at 2 x and 10 x MIC. Overall, these results suggest that VpDef has an important function in host defense against invasive pathogens, probably killing microbes by inducing membrane lesions. (C) 2015 Elsevier Ltd. All rights reserved. |
| 文章类型 | Article |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | OYSTER CRASSOSTREA-GIGAS ; RICH ANTIMICROBIAL PEPTIDES ; PRECURSOR LIPID II ; MARINE-INVERTEBRATES ; ESCHERICHIA-COLI ; RUDITAPES-PHILIPPINARUM ; MICROCOCCUS-LUTEUS ; ARTHROPOD DEFENSIN ; INNATE IMMUNITY ; MEMBRANE |
| 研究领域[WOS] | Immunology ; Zoology |
| WOS记录号 | WOS:000357906800004 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/9274 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 中国科学院海岸带环境过程与生态修复重点实验室_污染过程与控制实验室 海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心 |
| 通讯作者 | Wu, HF (reprint author), YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China. hfwu@yic.ac.cn; jmzhao@yic.ac.cn |
| 推荐引用方式 GB/T 7714 | Zhang, LB,Yang, DL,Wang, Q,et al. A defensin from clam Venerupis philippinarum: Molecular characterization, localization, antibacterial activity, and mechanism of action[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2015,51(1):29-38. |
| APA | Zhang, LB.,Yang, DL.,Wang, Q.,Yuan, ZY.,Wu, HF.,...&jmzhao@yic.ac.cn.(2015).A defensin from clam Venerupis philippinarum: Molecular characterization, localization, antibacterial activity, and mechanism of action.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,51(1),29-38. |
| MLA | Zhang, LB,et al."A defensin from clam Venerupis philippinarum: Molecular characterization, localization, antibacterial activity, and mechanism of action".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 51.1(2015):29-38. |
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