Digital gene expression analysis in the gills of Ruditapes philippinarum exposed to short- and long-term exposures of ammonia nitrogen
Cong, Ming; Wu, Huifeng; Cao, Tengfei; Lv, Jiasen; Wang, Qing; Ji, Chenglong; Li, Chenghua; Zhao, Jianmin
发表期刊AQUATIC TOXICOLOGY
ISSN0166-445X
2018-01
卷号194页码:121-131
关键词Ruditapes philippinarum Digital gene expression Transcriptome Ammonia nitrogen Toxicity
研究领域Marine & Freshwater Biology ; Toxicology
DOI10.1016/j.aquatox.2017.11.012
产权排序[Cong, Ming; Wu, Huifeng; Wang, Qing; Ji, Chenglong; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Wu, Huifeng] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China; [Cao, Tengfei; Li, Chenghua] Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China; [Lv, Jiasen] Yantai Univ, Biol Sch, Yantai 264005, Peoples R China
作者部门海岸带环境过程实验室
英文摘要Previous study revealed severe toxic effects of ammonia nitrogen on Ruditapes philippinanun including lysosomal instability, disturbed metabolic profiles, gill tissues with damaged structure, and variation of neurotransmitter concentrations. However, the underlying molecular mechanism was not fully understood yet. In the present study, digital gene expression technology (DGE) was applied to globally screen the key genes and pathways involved in the responses to short- and long-term exposures of ammonia nitrogen. Results of DGE analysis indicated that short-term duration of ammonia exposure affected pathways in Dorso-ventral axis formation, Notch signaling, thyroid hormone signaling and protein processing in endoplasmic reticulum. The long-term exposure led to DEGs significantly enriched in gap junction, immunity, signal and hormone transduction, as well as key substance metabolism pathways. Functional research of significantly changed DEGs suggested that the immunity of R. philippinarum was weakened heavily by toxic effects of ammonia nitrogen, as well as neuro-transduction and metabolism of important substances. Taken together, the present study provides a molecular support for the previous results of the detrimental toxicity of ammonia exposure in R. philippinarum, further work will be performed to investigate the specific genes and their certain functions involved in ammonia toxicity to molluscs.
文章类型Article
资助机构Natural Science Foundation of China [41406132] ; National Key Basic Research Program of China [2015CB453303] ; Youth Innovation Promotion Association CAS
收录类别SCI
语种英语
关键词[WOS]BINDING-PROPERTIES ; CHLAMYS-FARRERI ; TOXICITY ; CALCIUM ; FISH ; STABILITY ; RECEPTOR ; GENOME ; STRESS ; LECTIN
研究领域[WOS]Marine & Freshwater Biology ; Toxicology
WOS记录号WOS:000423644200014
引用统计
被引频次:30[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/24406
专题中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室
海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
中国科学院海岸带环境过程与生态修复重点实验室_污染过程与控制实验室
中国科学院海岸带环境过程与生态修复重点实验室
海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China;
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China;
3.Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China;
4.Yantai Univ, Biol Sch, Yantai 264005, Peoples R China
推荐引用方式
GB/T 7714
Cong, Ming,Wu, Huifeng,Cao, Tengfei,et al. Digital gene expression analysis in the gills of Ruditapes philippinarum exposed to short- and long-term exposures of ammonia nitrogen[J]. AQUATIC TOXICOLOGY,2018,194:121-131.
APA Cong, Ming.,Wu, Huifeng.,Cao, Tengfei.,Lv, Jiasen.,Wang, Qing.,...&Zhao, Jianmin.(2018).Digital gene expression analysis in the gills of Ruditapes philippinarum exposed to short- and long-term exposures of ammonia nitrogen.AQUATIC TOXICOLOGY,194,121-131.
MLA Cong, Ming,et al."Digital gene expression analysis in the gills of Ruditapes philippinarum exposed to short- and long-term exposures of ammonia nitrogen".AQUATIC TOXICOLOGY 194(2018):121-131.
条目包含的文件 下载所有文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Digital gene express(938KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 下载
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Cong, Ming]的文章
[Wu, Huifeng]的文章
[Cao, Tengfei]的文章
百度学术
百度学术中相似的文章
[Cong, Ming]的文章
[Wu, Huifeng]的文章
[Cao, Tengfei]的文章
必应学术
必应学术中相似的文章
[Cong, Ming]的文章
[Wu, Huifeng]的文章
[Cao, Tengfei]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Digital gene expression analysis in the gills of Ruditapes philippinarum exposed to short- and long-term exposures of ammonia nitrogen.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。