Digital gene expression analysis in the gills of Ruditapes philippinarum exposed to short- and long-term exposures of ammonia nitrogen
Cong, Ming; Wu, Huifeng; Cao, Tengfei; Lv, Jiasen; Wang, Qing; Ji, Chenglong; Li, Chenghua; Zhao, Jianmin
Source PublicationAQUATIC TOXICOLOGY
ISSN0166-445X
2018-01
Volume194Pages:121-131
KeywordRuditapes philippinarum Digital gene expression Transcriptome Ammonia nitrogen Toxicity
MOST Discipline CatalogueMarine & Freshwater Biology ; Toxicology
DOI10.1016/j.aquatox.2017.11.012
Contribution Rank[Cong, Ming; Wu, Huifeng; Wang, Qing; Ji, Chenglong; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Wu, Huifeng] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China; [Cao, Tengfei; Li, Chenghua] Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China; [Lv, Jiasen] Yantai Univ, Biol Sch, Yantai 264005, Peoples R China
Department海岸带环境过程实验室
AbstractPrevious study revealed severe toxic effects of ammonia nitrogen on Ruditapes philippinanun including lysosomal instability, disturbed metabolic profiles, gill tissues with damaged structure, and variation of neurotransmitter concentrations. However, the underlying molecular mechanism was not fully understood yet. In the present study, digital gene expression technology (DGE) was applied to globally screen the key genes and pathways involved in the responses to short- and long-term exposures of ammonia nitrogen. Results of DGE analysis indicated that short-term duration of ammonia exposure affected pathways in Dorso-ventral axis formation, Notch signaling, thyroid hormone signaling and protein processing in endoplasmic reticulum. The long-term exposure led to DEGs significantly enriched in gap junction, immunity, signal and hormone transduction, as well as key substance metabolism pathways. Functional research of significantly changed DEGs suggested that the immunity of R. philippinarum was weakened heavily by toxic effects of ammonia nitrogen, as well as neuro-transduction and metabolism of important substances. Taken together, the present study provides a molecular support for the previous results of the detrimental toxicity of ammonia exposure in R. philippinarum, further work will be performed to investigate the specific genes and their certain functions involved in ammonia toxicity to molluscs.
Funding OrganizationNatural Science Foundation of China [41406132] ; National Key Basic Research Program of China [2015CB453303] ; Youth Innovation Promotion Association CAS
SubtypeArticle
Indexed BySCI
Language英语
WOS KeywordBINDING-PROPERTIES ; CHLAMYS-FARRERI ; TOXICITY ; CALCIUM ; FISH ; STABILITY ; RECEPTOR ; GENOME ; STRESS ; LECTIN
WOS Research AreaMarine & Freshwater Biology ; Toxicology
WOS IDWOS:000423644200014
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/24406
Collection中科院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室
Affiliation1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China;
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China;
3.Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China;
4.Yantai Univ, Biol Sch, Yantai 264005, Peoples R China
Recommended Citation
GB/T 7714
Cong, Ming,Wu, Huifeng,Cao, Tengfei,et al. Digital gene expression analysis in the gills of Ruditapes philippinarum exposed to short- and long-term exposures of ammonia nitrogen[J]. AQUATIC TOXICOLOGY,2018,194:121-131.
APA Cong, Ming.,Wu, Huifeng.,Cao, Tengfei.,Lv, Jiasen.,Wang, Qing.,...&Zhao, Jianmin.(2018).Digital gene expression analysis in the gills of Ruditapes philippinarum exposed to short- and long-term exposures of ammonia nitrogen.AQUATIC TOXICOLOGY,194,121-131.
MLA Cong, Ming,et al."Digital gene expression analysis in the gills of Ruditapes philippinarum exposed to short- and long-term exposures of ammonia nitrogen".AQUATIC TOXICOLOGY 194(2018):121-131.
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