YIC-IR  > 中科院海岸带环境过程与生态修复重点实验室
Metabolic profiling of the tissue-specific responses in mussel Mytilus galloprovincialis towards Vibrio harveyi challenge
Liu, Xiaoli1; Ji, Chenglong2; Zhao, Jianmin2; Wang, Qing2; Li, Fei2; Wu, Huifeng2; Liu, XL (reprint author), Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China. zlylx12009@gmail.com
2014-08-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume39Issue:2Pages:372-377
Contribution Rank[Liu, Xiaoli] Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China; [Ji, Chenglong; Zhao, Jianmin; Wang, Qing; Li, Fei; Wu, Huifeng] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Lab Coastal Ecotoxicol, Yantai 264003, Peoples R China
AbstractMussel Mytilus galloprovincialis is a marine aquaculture shellfish distributing widely along the coast in north China. In this work, we studied the differential metabolic responses induced by Vibrio harveyi in digestive gland and gill tissues from M. galloprovincialis using NMR-based metabolomics. The differential metabolic responses in the two tissue types were detected, except the similarly altered taurine and betaine. These metabolic responses suggested that V. harveyi mainly induced osmotic disruption and reduced energy demand via the metabolic pathways of glucose synthesis and ATP/AMP conversion in mussel digestive gland. In mussel gill tissues, V. harveyi basically caused osmotic stress and possible reduced energy demand as shown by the elevated phosphocholine that is involved in one of the metabolic pathways of ATP synthesis from ADP and phosphocholine. The altered mRNA expression levels of related genes (superoxide dismutase with copper and zinc, heat shock protein 90, defensin and lysozyme) suggested that V. harveyi induced clear oxidative and immune stresses in both digestive gland and gill tissues. However, the mRNA expression levels of both lysozyme and defensin in digestive gland were more significantly up-regulated than those in gill from V harveyi-challenged mussel M. galloprovincialis, meaning that the immune organ, digestive gland, was more sensitive than gill. Overall, our results indicated that V. harveyi could induce tissue-specific metabolic responses in mussel M. galloprovincialis. (C) 2014 Elsevier Ltd. All rights reserved.; Mussel Mytilus galloprovincialis is a marine aquaculture shellfish distributing widely along the coast in north China. In this work, we studied the differential metabolic responses induced by Vibrio harveyi in digestive gland and gill tissues from M. galloprovincialis using NMR-based metabolomics. The differential metabolic responses in the two tissue types were detected, except the similarly altered taurine and betaine. These metabolic responses suggested that V. harveyi mainly induced osmotic disruption and reduced energy demand via the metabolic pathways of glucose synthesis and ATP/AMP conversion in mussel digestive gland. In mussel gill tissues, V. harveyi basically caused osmotic stress and possible reduced energy demand as shown by the elevated phosphocholine that is involved in one of the metabolic pathways of ATP synthesis from ADP and phosphocholine. The altered mRNA expression levels of related genes (superoxide dismutase with copper and zinc, heat shock protein 90, defensin and lysozyme) suggested that V. harveyi induced clear oxidative and immune stresses in both digestive gland and gill tissues. However, the mRNA expression levels of both lysozyme and defensin in digestive gland were more significantly up-regulated than those in gill from V harveyi-challenged mussel M. galloprovincialis, meaning that the immune organ, digestive gland, was more sensitive than gill. Overall, our results indicated that V. harveyi could induce tissue-specific metabolic responses in mussel M. galloprovincialis. (C) 2014 Elsevier Ltd. All rights reserved.
KeywordMetabolomics Mytilus Galloprovincialis Digestive Gland Gill Vibrio Harveyi
Department中科院海岸带环境过程与生态修复重点实验室
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI10.1016/j.fsi.2014.05.033
Funding OrganizationFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
Indexed BySCI
WOS KeywordCLAM RUDITAPES-PHILIPPINARUM ; GENE-EXPRESSION ; MEDITERRANEAN MUSSEL ; PATTERN-RECOGNITION ; NMR METABOLOMICS ; ANGUILLARUM ; HEMOCYTES ; PATHOGEN ; SPECTROSCOPY ; LA(NO3)(3)
SubtypeArticle
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000339773000032
Citation statistics
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/8723
Collection中科院海岸带环境过程与生态修复重点实验室
Corresponding AuthorLiu, XL (reprint author), Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China. zlylx12009@gmail.com
Affiliation1.Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Lab Coastal Ecotoxicol, Yantai 264003, Peoples R China
Recommended Citation
GB/T 7714
Liu, Xiaoli,Ji, Chenglong,Zhao, Jianmin,et al. Metabolic profiling of the tissue-specific responses in mussel Mytilus galloprovincialis towards Vibrio harveyi challenge[J]. FISH & SHELLFISH IMMUNOLOGY,2014,39(2):372-377.
APA Liu, Xiaoli.,Ji, Chenglong.,Zhao, Jianmin.,Wang, Qing.,Li, Fei.,...&Liu, XL .(2014).Metabolic profiling of the tissue-specific responses in mussel Mytilus galloprovincialis towards Vibrio harveyi challenge.FISH & SHELLFISH IMMUNOLOGY,39(2),372-377.
MLA Liu, Xiaoli,et al."Metabolic profiling of the tissue-specific responses in mussel Mytilus galloprovincialis towards Vibrio harveyi challenge".FISH & SHELLFISH IMMUNOLOGY 39.2(2014):372-377.
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