Institutional Repository of Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences (KLCEP)
| Metabolic profiling of the tissue-specific responses in mussel Mytilus galloprovincialis towards Vibrio harveyi challenge | |
Liu, Xiaoli1; Ji, Chenglong2 ; Zhao, Jianmin2; Wang, Qing2; Li, Fei2; Wu, Huifeng2; Liu, XL (reprint author), Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China. zlylx12009@gmail.com
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| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| 2014-08-01 | |
| 卷号 | 39期号:2页码:372-377 |
| 关键词 | Metabolomics Mytilus Galloprovincialis Digestive Gland Gill Vibrio Harveyi |
| DOI | 10.1016/j.fsi.2014.05.033 |
| 产权排序 | [Liu, Xiaoli] Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China; [Ji, Chenglong; Zhao, Jianmin; Wang, Qing; Li, Fei; Wu, Huifeng] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Lab Coastal Ecotoxicol, Yantai 264003, Peoples R China |
| 作者部门 | 中科院海岸带环境过程与生态修复重点实验室 |
| 英文摘要 | Mussel Mytilus galloprovincialis is a marine aquaculture shellfish distributing widely along the coast in north China. In this work, we studied the differential metabolic responses induced by Vibrio harveyi in digestive gland and gill tissues from M. galloprovincialis using NMR-based metabolomics. The differential metabolic responses in the two tissue types were detected, except the similarly altered taurine and betaine. These metabolic responses suggested that V. harveyi mainly induced osmotic disruption and reduced energy demand via the metabolic pathways of glucose synthesis and ATP/AMP conversion in mussel digestive gland. In mussel gill tissues, V. harveyi basically caused osmotic stress and possible reduced energy demand as shown by the elevated phosphocholine that is involved in one of the metabolic pathways of ATP synthesis from ADP and phosphocholine. The altered mRNA expression levels of related genes (superoxide dismutase with copper and zinc, heat shock protein 90, defensin and lysozyme) suggested that V. harveyi induced clear oxidative and immune stresses in both digestive gland and gill tissues. However, the mRNA expression levels of both lysozyme and defensin in digestive gland were more significantly up-regulated than those in gill from V harveyi-challenged mussel M. galloprovincialis, meaning that the immune organ, digestive gland, was more sensitive than gill. Overall, our results indicated that V. harveyi could induce tissue-specific metabolic responses in mussel M. galloprovincialis. (C) 2014 Elsevier Ltd. All rights reserved.; Mussel Mytilus galloprovincialis is a marine aquaculture shellfish distributing widely along the coast in north China. In this work, we studied the differential metabolic responses induced by Vibrio harveyi in digestive gland and gill tissues from M. galloprovincialis using NMR-based metabolomics. The differential metabolic responses in the two tissue types were detected, except the similarly altered taurine and betaine. These metabolic responses suggested that V. harveyi mainly induced osmotic disruption and reduced energy demand via the metabolic pathways of glucose synthesis and ATP/AMP conversion in mussel digestive gland. In mussel gill tissues, V. harveyi basically caused osmotic stress and possible reduced energy demand as shown by the elevated phosphocholine that is involved in one of the metabolic pathways of ATP synthesis from ADP and phosphocholine. The altered mRNA expression levels of related genes (superoxide dismutase with copper and zinc, heat shock protein 90, defensin and lysozyme) suggested that V. harveyi induced clear oxidative and immune stresses in both digestive gland and gill tissues. However, the mRNA expression levels of both lysozyme and defensin in digestive gland were more significantly up-regulated than those in gill from V harveyi-challenged mussel M. galloprovincialis, meaning that the immune organ, digestive gland, was more sensitive than gill. Overall, our results indicated that V. harveyi could induce tissue-specific metabolic responses in mussel M. galloprovincialis. (C) 2014 Elsevier Ltd. All rights reserved. |
| 文章类型 | Article |
| 资助机构 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | CLAM RUDITAPES-PHILIPPINARUM ; GENE-EXPRESSION ; MEDITERRANEAN MUSSEL ; PATTERN-RECOGNITION ; NMR METABOLOMICS ; ANGUILLARUM ; HEMOCYTES ; PATHOGEN ; SPECTROSCOPY ; LA(NO3)(3) |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS记录号 | WOS:000339773000032 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/8723 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 通讯作者 | Liu, XL (reprint author), Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China. zlylx12009@gmail.com |
| 作者单位 | 1.Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Lab Coastal Ecotoxicol, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Xiaoli,Ji, Chenglong,Zhao, Jianmin,et al. Metabolic profiling of the tissue-specific responses in mussel Mytilus galloprovincialis towards Vibrio harveyi challenge[J]. FISH & SHELLFISH IMMUNOLOGY,2014,39(2):372-377. |
| APA | Liu, Xiaoli.,Ji, Chenglong.,Zhao, Jianmin.,Wang, Qing.,Li, Fei.,...&Liu, XL .(2014).Metabolic profiling of the tissue-specific responses in mussel Mytilus galloprovincialis towards Vibrio harveyi challenge.FISH & SHELLFISH IMMUNOLOGY,39(2),372-377. |
| MLA | Liu, Xiaoli,et al."Metabolic profiling of the tissue-specific responses in mussel Mytilus galloprovincialis towards Vibrio harveyi challenge".FISH & SHELLFISH IMMUNOLOGY 39.2(2014):372-377. |
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