Proteomic and metabolomic responses of Pacific oyster Crassostrea gigas to elevated pCO(2) exposure

doi:10.1016/j.jprot.2014.08.010

	Proteomic and metabolomic responses of Pacific oyster Crassostrea gigas to elevated pCO(2) exposure
	Wei, Lei; Wang, Qing; Wu, Huifeng; Ji, Chenglong; Zhao, Jianmin; Wu, HF (reprint author), Chinese Acad Sci, YICCAS, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc,Shandong Prov, Yantai 264003, Peoples R China. hfwu@yic.ac.cn; jmzhao@yic.ac.cn
发表期刊	JOURNAL OF PROTEOMICS
ISSN	1874-3919
	2015
卷号	112 页码:83-94
关键词	Ocean Acidification Proteomics Metabolomics Oyster Crassostrea Gigas
DOI	10.1016/j.jprot.2014.08.010
产权排序	[Wei, Lei; Wang, Qing; Wu, Huifeng; Ji, Chenglong; Zhao, Jianmin] Chinese Acad Sci, YICCAS, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc,Shandong Prov, Yantai 264003, Peoples R China; [Wei, Lei] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
作者部门	中科院海岸带环境过程与生态修复重点实验室
英文摘要	The gradually increased atmospheric CO2 partial pressure (pCO(2)) has thrown the carbonate chemistry off balance and resulted in decreased seawater pH in marine ecosystem, termed ocean acidification (OA). Anthropogenic OA is postulated to affect the physiology of many marine calcifying organisms. However, the susceptibility and metabolic pathways of change in most calcifying animals are still far from being well understood. In this work, the effects of exposure to elevated pCO(2) were characterized in gills and hepatopancreas of Crassostrea gigas using integrated proteomic and metabolomic approaches. Metabolic responses indicated that high CO2 exposure mainly caused disturbances in energy metabolism and osmotic regulation marked by differentially altered ATP, glucose, glycogen, amino acids and organic osmolytes in oysters, and the depletions of ATP in gills and the accumulations of ATP, glucose and glycogen in hepatopancreas accounted for the difference in energy distribution between these two tissues. Proteomic responses suggested that OA could not only affect energy and primary metabolisms, stress responses and calcium homeostasis in both tissues, but also influence the nucleotide metabolism in gills and cytoskeleton structure in hepatopancreas. This study demonstrated that the combination of proteomics and metabolomics could provide an insightful view into the effects of OA on oyster C. gigas. Biological significance The gradually increased atmospheric CO2 partial pressure (pCO(2)) has thrown the carbonate chemistry off balance and resulted in decreased seawater pH in marine ecosystem, termed ocean acidification (OA). Anthropogenic OA is postulated to affect the physiology of many marine calcifying organisms. However, the susceptibility and metabolic pathways of change in most calcifying animals are still far from being understood. To our knowledge, few studies have focused on the responses induced by pCO(2) at both protein and metabolite levels. The pacific oyster C. gigas, widely distributed throughout most of the world's oceans, is a model organism for marine environmental science. In the present study, an integrated metabolomic and proteomic approach was used to elucidate the effects of ocean acidification on Pacific oyster C. gigas, hopefully shedding light on the physiological responses of marine mollusk to the OA stress. (C) 2014 Elsevier B.V. All rights reserved.
文章类型	期刊论文
资助机构	Biochemistry & Molecular Biology
收录类别	SCI
语种	英语
关键词[WOS]	LASER-DESORPTION/IONIZATION-TIME ; CLAM RUDITAPES-PHILIPPINARUM ; OCEAN ACIDIFICATION ; ARGININE KINASE ; TROPONIN-C ; TRYPANOSOMA-CRUZI ; OXIDATIVE STRESS ; PROTEIN ; GENE ; CARBON
研究领域[WOS]	National Natural Science Foundation of China [31172388] ; Key Research Program of the Chinese Academy of Sciences [KZZD-EW-14]
WOS记录号	WOS:000347021100007
引用统计	被引频次：49[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/8677
专题	中国科学院海岸带环境过程与生态修复重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者	Wu, HF (reprint author), Chinese Acad Sci, YICCAS, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc,Shandong Prov, Yantai 264003, Peoples R China. hfwu@yic.ac.cn; jmzhao@yic.ac.cn
推荐引用方式 GB/T 7714	Wei, Lei,Wang, Qing,Wu, Huifeng,et al. Proteomic and metabolomic responses of Pacific oyster Crassostrea gigas to elevated pCO(2) exposure[J]. JOURNAL OF PROTEOMICS,2015,112:83-94.
APA	Wei, Lei.,Wang, Qing.,Wu, Huifeng.,Ji, Chenglong.,Zhao, Jianmin.,...&jmzhao@yic.ac.cn.(2015).Proteomic and metabolomic responses of Pacific oyster Crassostrea gigas to elevated pCO(2) exposure.JOURNAL OF PROTEOMICS,112,83-94.
MLA	Wei, Lei,et al."Proteomic and metabolomic responses of Pacific oyster Crassostrea gigas to elevated pCO(2) exposure".JOURNAL OF PROTEOMICS 112(2015):83-94.

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