YIC-IR  > 中科院海岸带环境过程与生态修复重点实验室
Responses of Mytilus galloprovincialis to bacterial challenges by metabolomics and proteomics
Alternative TitleUltrasensitive surface-enhanced Raman scattering detection of trypsin based on .pdf
Ji, Chenglong1,2; Wu, Huifeng1; Wei, Lei1,2; Zhao, Jianmin1; Wang, Qing1; Lu, Hongjian3
2013-08-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume35Issue:2Pages:489-498
Contribution Rank[Ji, Chenglong; Wu, Huifeng; Wei, Lei; Zhao, Jianmin; Wang, Qing] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc,YICCAS, Yantai 264003, Peoples R China; [Ji, Chenglong; Wei, Lei] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China; [Lu, Hongjian] Nanjing Univ, Inst Chem & BioMed Sci, Nanjing 210046, Jiangsu, Peoples R China
AbstractPathogens can cause diseases and lead to massive mortalities of aquaculture animals and substantial economic loss. In this work, we studied the responses induced by Micrococcus luteus and Vibrio anguillarum in gill of mussel Mytilus galloprovincialis at protein and metabolite levels. Metabolic biomarkers (e.g., amino acids, betaine, ATP) suggested that both M. luteus and V. anguillarum induced disturbances in energy metabolism and osmotic regulation. The unique and some more remarkably altered metabolic biomarkers (threonine, alanine, aspartate, taurine, succinate) demonstrated that V anguillarum could cause more severe disturbances in osmotic regulation and energy metabolism. Proteomic biomarkers (e.g., goose-type lysozyme 2, matrilin, ependymin-related protein, peptidylprolyl cis-trans isomerases) indicated that M. luteus caused immune stress, and disturbances in signaling pathways and protein synthesis. However, V anguillarum mainly induced oxidative stress and disturbance in energy metabolism in mussel gills indicated by altered procollagen-proline dioxygenase, protein disulfide isomerase, nucleoside diphosphate kinases, electron transfer flavoprotein and glutathione S-transferase. This work confirmed that an integration of proteomics and metabolomics could provide an insightful view into the effects of pathogens to the marine mussel M. galloprovincialis. (C) 2013 Elsevier Ltd. All rights reserved.; Pathogens can cause diseases and lead to massive mortalities of aquaculture animals and substantial economic loss. In this work, we studied the responses induced by Micrococcus luteus and Vibrio anguillarum in gill of mussel Mytilus galloprovincialis at protein and metabolite levels. Metabolic biomarkers (e.g., amino acids, betaine, ATP) suggested that both M. luteus and V. anguillarum induced disturbances in energy metabolism and osmotic regulation. The unique and some more remarkably altered metabolic biomarkers (threonine, alanine, aspartate, taurine, succinate) demonstrated that V anguillarum could cause more severe disturbances in osmotic regulation and energy metabolism. Proteomic biomarkers (e.g., goose-type lysozyme 2, matrilin, ependymin-related protein, peptidylprolyl cis-trans isomerases) indicated that M. luteus caused immune stress, and disturbances in signaling pathways and protein synthesis. However, V anguillarum mainly induced oxidative stress and disturbance in energy metabolism in mussel gills indicated by altered procollagen-proline dioxygenase, protein disulfide isomerase, nucleoside diphosphate kinases, electron transfer flavoprotein and glutathione S-transferase. This work confirmed that an integration of proteomics and metabolomics could provide an insightful view into the effects of pathogens to the marine mussel M. galloprovincialis. (C) 2013 Elsevier Ltd. All rights reserved.
Corresponding AuthorWu, HF (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc,YICCAS, Yantai 264003, Peoples R China. hfwu@yic.ac.cn
KeywordProteomics Metabolomics Bacteria Mytilus Galloprovincialis Gill
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
Funding OrganizationChinese Academy of Sciences
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Indexed BySCI
WOS KeywordLASER-DESORPTION/IONIZATION-TIME ; NUCLEAR-MAGNETIC-RESONANCE ; HIGH-FAT DIET ; MEDITERRANEAN MUSSEL ; CRASSOSTREA-GIGAS ; STRESS RESPONSES ; NMR METABOLOMICS ; IMMUNE-RESPONSE ; GENE-EXPRESSION ; MARINE BIVALVES
SubtypeArticle
Language英语
Funding Project中国科学院海岸带环境过程与生态修复重点实验室
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000322207500035
Citation statistics
Cited Times:47[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/6966
Collection中科院海岸带环境过程与生态修复重点实验室
Affiliation1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc,YICCAS, Yantai 264003, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
3.Nanjing Univ, Inst Chem & BioMed Sci, Nanjing 210046, Jiangsu, Peoples R China
First Author AffilicationYantai Institute of Coastal Zone Research, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Ji, Chenglong,Wu, Huifeng,Wei, Lei,et al. Responses of Mytilus galloprovincialis to bacterial challenges by metabolomics and proteomics[J]. FISH & SHELLFISH IMMUNOLOGY,2013,35(2):489-498.
APA Ji, Chenglong,Wu, Huifeng,Wei, Lei,Zhao, Jianmin,Wang, Qing,&Lu, Hongjian.(2013).Responses of Mytilus galloprovincialis to bacterial challenges by metabolomics and proteomics.FISH & SHELLFISH IMMUNOLOGY,35(2),489-498.
MLA Ji, Chenglong,et al."Responses of Mytilus galloprovincialis to bacterial challenges by metabolomics and proteomics".FISH & SHELLFISH IMMUNOLOGY 35.2(2013):489-498.
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