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| Responses of Mytilus galloprovincialis to bacterial challenges by metabolomics and proteomics | |
| 其他题名 | Ultrasensitive surface-enhanced Raman scattering detection of trypsin based on .pdf |
Ji, Chenglong1,2 ; Wu, Huifeng1; Wei, Lei1,2; Zhao, Jianmin1; Wang, Qing1; Lu, Hongjian3
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| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| 2013-08-01 | |
| 卷号 | 35期号:2页码:489-498 |
| 关键词 | Proteomics Metabolomics Bacteria Mytilus Galloprovincialis Gill |
| 产权排序 | [Ji, Chenglong; Wu, Huifeng; Wei, Lei; Zhao, Jianmin; Wang, Qing] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc,YICCAS, Yantai 264003, Peoples R China; [Ji, Chenglong; Wei, Lei] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China; [Lu, Hongjian] Nanjing Univ, Inst Chem & BioMed Sci, Nanjing 210046, Jiangsu, Peoples R China |
| 通讯作者 | Wu, HF (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc,YICCAS, Yantai 264003, Peoples R China. hfwu@yic.ac.cn |
| 英文摘要 | Pathogens can cause diseases and lead to massive mortalities of aquaculture animals and substantial economic loss. In this work, we studied the responses induced by Micrococcus luteus and Vibrio anguillarum in gill of mussel Mytilus galloprovincialis at protein and metabolite levels. Metabolic biomarkers (e.g., amino acids, betaine, ATP) suggested that both M. luteus and V. anguillarum induced disturbances in energy metabolism and osmotic regulation. The unique and some more remarkably altered metabolic biomarkers (threonine, alanine, aspartate, taurine, succinate) demonstrated that V anguillarum could cause more severe disturbances in osmotic regulation and energy metabolism. Proteomic biomarkers (e.g., goose-type lysozyme 2, matrilin, ependymin-related protein, peptidylprolyl cis-trans isomerases) indicated that M. luteus caused immune stress, and disturbances in signaling pathways and protein synthesis. However, V anguillarum mainly induced oxidative stress and disturbance in energy metabolism in mussel gills indicated by altered procollagen-proline dioxygenase, protein disulfide isomerase, nucleoside diphosphate kinases, electron transfer flavoprotein and glutathione S-transferase. This work confirmed that an integration of proteomics and metabolomics could provide an insightful view into the effects of pathogens to the marine mussel M. galloprovincialis. (C) 2013 Elsevier Ltd. All rights reserved.; Pathogens can cause diseases and lead to massive mortalities of aquaculture animals and substantial economic loss. In this work, we studied the responses induced by Micrococcus luteus and Vibrio anguillarum in gill of mussel Mytilus galloprovincialis at protein and metabolite levels. Metabolic biomarkers (e.g., amino acids, betaine, ATP) suggested that both M. luteus and V. anguillarum induced disturbances in energy metabolism and osmotic regulation. The unique and some more remarkably altered metabolic biomarkers (threonine, alanine, aspartate, taurine, succinate) demonstrated that V anguillarum could cause more severe disturbances in osmotic regulation and energy metabolism. Proteomic biomarkers (e.g., goose-type lysozyme 2, matrilin, ependymin-related protein, peptidylprolyl cis-trans isomerases) indicated that M. luteus caused immune stress, and disturbances in signaling pathways and protein synthesis. However, V anguillarum mainly induced oxidative stress and disturbance in energy metabolism in mussel gills indicated by altered procollagen-proline dioxygenase, protein disulfide isomerase, nucleoside diphosphate kinases, electron transfer flavoprotein and glutathione S-transferase. This work confirmed that an integration of proteomics and metabolomics could provide an insightful view into the effects of pathogens to the marine mussel M. galloprovincialis. (C) 2013 Elsevier Ltd. All rights reserved. |
| 文章类型 | Article |
| 资助机构 | Chinese Academy of Sciences |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | LASER-DESORPTION/IONIZATION-TIME ; NUCLEAR-MAGNETIC-RESONANCE ; HIGH-FAT DIET ; MEDITERRANEAN MUSSEL ; CRASSOSTREA-GIGAS ; STRESS RESPONSES ; NMR METABOLOMICS ; IMMUNE-RESPONSE ; GENE-EXPRESSION ; MARINE BIVALVES |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS记录号 | WOS:000322207500035 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/6966 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc,YICCAS, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Nanjing Univ, Inst Chem & BioMed Sci, Nanjing 210046, Jiangsu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ji, Chenglong,Wu, Huifeng,Wei, Lei,et al. Responses of Mytilus galloprovincialis to bacterial challenges by metabolomics and proteomics[J]. FISH & SHELLFISH IMMUNOLOGY,2013,35(2):489-498. |
| APA | Ji, Chenglong,Wu, Huifeng,Wei, Lei,Zhao, Jianmin,Wang, Qing,&Lu, Hongjian.(2013).Responses of Mytilus galloprovincialis to bacterial challenges by metabolomics and proteomics.FISH & SHELLFISH IMMUNOLOGY,35(2),489-498. |
| MLA | Ji, Chenglong,et al."Responses of Mytilus galloprovincialis to bacterial challenges by metabolomics and proteomics".FISH & SHELLFISH IMMUNOLOGY 35.2(2013):489-498. |
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