YIC-IR  > 中科院海岸带环境过程与生态修复重点实验室
The response profiles of HSPA12A and TCTP from Mytilus galloprovincialis to pathogen and cadmium challenge
You, Liping1,3; Ning, Xuanxuan2; Liu, Feng4; Zhao, Jianmin1; Wang, Qing1; Wu, Huifeng1
2013-08-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume35Issue:2Pages:343-350
Contribution Rank[You, Liping; Zhao, Jianmin; Wang, Qing; Wu, Huifeng] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Processes & Ecol Re, Yantai 264003, Peoples R China; [Ning, Xuanxuan] State Ocean Adm, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China; [You, Liping] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Liu, Feng] China Agr Univ, Yantai 264670, Peoples R China
AbstractHeat shock 70 kDa protein 12A (HSPA12A) is an atypical member of HSP70 family, and the translationally controlled tumor protein (TCTP) is a novel HSP with chaperone-like activity. They are both involved in protecting organisms against various stressors. In the present study, the cDNAs of HSPA12A and TCTP (called MgHSPA12A and MgTCTP) were identified from Mytilus galloprovincialis by RACE approaches. The full-length cDNA of MgHSPA12A and MgTCTP encoded a peptide of 491 and 171 amino acids, respectively. Real-time PCR was employed to analyze the tissue distribution and temporal expression of these two genes after bacterial challenge and cadmium (Cd) exposure. It was found that the transcripts of MgHSPA12A and MgTCTP were dominantly expressed in gonad and muscle, respectively. The expression level of MgTCTP at 48 h post Vibrio anguillarum challenge was detected to be significantly up-regulated in hepatopancreas (P < 0.05). As concerned to Cd exposure, 2.0-fold increase of MgHSPA12A expression compared to that of the control was observed at 48 h in 5 mu g/L Cd2+-treated group, while the expression levels of MgTCTP were significantly decreased after exposed to both 5 and 50 mu g/L Cd2+ for 24 h and 96 h. These results suggested the potential involvement of MgHSPA12A and MgTCTP in the mediation of the immune responses and environmental stress in mussels. (C) 2013 Elsevier Ltd. All rights reserved.; Heat shock 70 kDa protein 12A (HSPA12A) is an atypical member of HSP70 family, and the translationally controlled tumor protein (TCTP) is a novel HSP with chaperone-like activity. They are both involved in protecting organisms against various stressors. In the present study, the cDNAs of HSPA12A and TCTP (called MgHSPA12A and MgTCTP) were identified from Mytilus galloprovincialis by RACE approaches. The full-length cDNA of MgHSPA12A and MgTCTP encoded a peptide of 491 and 171 amino acids, respectively. Real-time PCR was employed to analyze the tissue distribution and temporal expression of these two genes after bacterial challenge and cadmium (Cd) exposure. It was found that the transcripts of MgHSPA12A and MgTCTP were dominantly expressed in gonad and muscle, respectively. The expression level of MgTCTP at 48 h post Vibrio anguillarum challenge was detected to be significantly up-regulated in hepatopancreas (P < 0.05). As concerned to Cd exposure, 2.0-fold increase of MgHSPA12A expression compared to that of the control was observed at 48 h in 5 mu g/L Cd2+-treated group, while the expression levels of MgTCTP were significantly decreased after exposed to both 5 and 50 mu g/L Cd2+ for 24 h and 96 h. These results suggested the potential involvement of MgHSPA12A and MgTCTP in the mediation of the immune responses and environmental stress in mussels. (C) 2013 Elsevier Ltd. All rights reserved.
Corresponding AuthorZhao, JM (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Processes & Ecol Re, Yantai 264003, Peoples R China. jmzhao@yic.ac.cn ; hfwu@yic.ac.cn
KeywordMytilus Galloprovincialis Heat Shock Proteins Gene Expression Vibrio Anguillarum Cadmium
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
Funding OrganizationNational Natural Science Foundation of China [31172388]; Chinese Academy of Sciences [KZZD-EW-14-03]
URL查看原文
Indexed BySCI
WOS KeywordCONTROLLED TUMOR PROTEIN ; HEAT-SHOCK-PROTEIN ; VENERUPIS-PHILIPPINARUM ; VIBRIO-SPLENDIDUS ; GENE-EXPRESSION ; MUSSELS ; STRESS ; IDENTIFICATION ; CHAPERONES ; HEMOCYTES
SubtypeArticle
Language英语
Funding Project中国科学院海岸带环境过程与生态修复重点实验室
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000322207500017
Citation statistics
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/6965
Collection中科院海岸带环境过程与生态修复重点实验室
Affiliation1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Processes & Ecol Re, Yantai 264003, Peoples R China
2.State Ocean Adm, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.China Agr Univ, Yantai 264670, Peoples R China
First Author AffilicationYantai Institute of Coastal Zone Research, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
You, Liping,Ning, Xuanxuan,Liu, Feng,et al. The response profiles of HSPA12A and TCTP from Mytilus galloprovincialis to pathogen and cadmium challenge[J]. FISH & SHELLFISH IMMUNOLOGY,2013,35(2):343-350.
APA You, Liping,Ning, Xuanxuan,Liu, Feng,Zhao, Jianmin,Wang, Qing,&Wu, Huifeng.(2013).The response profiles of HSPA12A and TCTP from Mytilus galloprovincialis to pathogen and cadmium challenge.FISH & SHELLFISH IMMUNOLOGY,35(2),343-350.
MLA You, Liping,et al."The response profiles of HSPA12A and TCTP from Mytilus galloprovincialis to pathogen and cadmium challenge".FISH & SHELLFISH IMMUNOLOGY 35.2(2013):343-350.
Files in This Item: Download All
File Name/Size DocType Version Access License
The response profile(1585KB) 开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[You, Liping]'s Articles
[Ning, Xuanxuan]'s Articles
[Liu, Feng]'s Articles
Baidu academic
Similar articles in Baidu academic
[You, Liping]'s Articles
[Ning, Xuanxuan]'s Articles
[Liu, Feng]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[You, Liping]'s Articles
[Ning, Xuanxuan]'s Articles
[Liu, Feng]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: The response profiles of HSPA12A and TCTP from Mytilus galloprovincialis to pathogen and cadmium challenge.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.