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| The response profiles of HSPA12A and TCTP from Mytilus galloprovincialis to pathogen and cadmium challenge | |
You, Liping1,3; Ning, Xuanxuan2; Liu, Feng4; Zhao, Jianmin1 ; Wang, Qing1; Wu, Huifeng1
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| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| 2013-08-01 | |
| 卷号 | 35期号:2页码:343-350 |
| 关键词 | Mytilus Galloprovincialis Heat Shock Proteins Gene Expression Vibrio Anguillarum Cadmium |
| 产权排序 | [You, Liping; Zhao, Jianmin; Wang, Qing; Wu, Huifeng] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Processes & Ecol Re, Yantai 264003, Peoples R China; [Ning, Xuanxuan] State Ocean Adm, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China; [You, Liping] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Liu, Feng] China Agr Univ, Yantai 264670, Peoples R China |
| 通讯作者 | Zhao, JM (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Processes & Ecol Re, Yantai 264003, Peoples R China. jmzhao@yic.ac.cn ; hfwu@yic.ac.cn |
| 英文摘要 | Heat shock 70 kDa protein 12A (HSPA12A) is an atypical member of HSP70 family, and the translationally controlled tumor protein (TCTP) is a novel HSP with chaperone-like activity. They are both involved in protecting organisms against various stressors. In the present study, the cDNAs of HSPA12A and TCTP (called MgHSPA12A and MgTCTP) were identified from Mytilus galloprovincialis by RACE approaches. The full-length cDNA of MgHSPA12A and MgTCTP encoded a peptide of 491 and 171 amino acids, respectively. Real-time PCR was employed to analyze the tissue distribution and temporal expression of these two genes after bacterial challenge and cadmium (Cd) exposure. It was found that the transcripts of MgHSPA12A and MgTCTP were dominantly expressed in gonad and muscle, respectively. The expression level of MgTCTP at 48 h post Vibrio anguillarum challenge was detected to be significantly up-regulated in hepatopancreas (P < 0.05). As concerned to Cd exposure, 2.0-fold increase of MgHSPA12A expression compared to that of the control was observed at 48 h in 5 mu g/L Cd2+-treated group, while the expression levels of MgTCTP were significantly decreased after exposed to both 5 and 50 mu g/L Cd2+ for 24 h and 96 h. These results suggested the potential involvement of MgHSPA12A and MgTCTP in the mediation of the immune responses and environmental stress in mussels. (C) 2013 Elsevier Ltd. All rights reserved.; Heat shock 70 kDa protein 12A (HSPA12A) is an atypical member of HSP70 family, and the translationally controlled tumor protein (TCTP) is a novel HSP with chaperone-like activity. They are both involved in protecting organisms against various stressors. In the present study, the cDNAs of HSPA12A and TCTP (called MgHSPA12A and MgTCTP) were identified from Mytilus galloprovincialis by RACE approaches. The full-length cDNA of MgHSPA12A and MgTCTP encoded a peptide of 491 and 171 amino acids, respectively. Real-time PCR was employed to analyze the tissue distribution and temporal expression of these two genes after bacterial challenge and cadmium (Cd) exposure. It was found that the transcripts of MgHSPA12A and MgTCTP were dominantly expressed in gonad and muscle, respectively. The expression level of MgTCTP at 48 h post Vibrio anguillarum challenge was detected to be significantly up-regulated in hepatopancreas (P < 0.05). As concerned to Cd exposure, 2.0-fold increase of MgHSPA12A expression compared to that of the control was observed at 48 h in 5 mu g/L Cd2+-treated group, while the expression levels of MgTCTP were significantly decreased after exposed to both 5 and 50 mu g/L Cd2+ for 24 h and 96 h. These results suggested the potential involvement of MgHSPA12A and MgTCTP in the mediation of the immune responses and environmental stress in mussels. (C) 2013 Elsevier Ltd. All rights reserved. |
| 文章类型 | Article |
| 资助机构 | National Natural Science Foundation of China [31172388]; Chinese Academy of Sciences [KZZD-EW-14-03] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | CONTROLLED TUMOR PROTEIN ; HEAT-SHOCK-PROTEIN ; VENERUPIS-PHILIPPINARUM ; VIBRIO-SPLENDIDUS ; GENE-EXPRESSION ; MUSSELS ; STRESS ; IDENTIFICATION ; CHAPERONES ; HEMOCYTES |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS记录号 | WOS:000322207500017 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/6965 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Processes & Ecol Re, Yantai 264003, Peoples R China 2.State Ocean Adm, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.China Agr Univ, Yantai 264670, Peoples R China |
| 推荐引用方式 GB/T 7714 | You, Liping,Ning, Xuanxuan,Liu, Feng,et al. The response profiles of HSPA12A and TCTP from Mytilus galloprovincialis to pathogen and cadmium challenge[J]. FISH & SHELLFISH IMMUNOLOGY,2013,35(2):343-350. |
| APA | You, Liping,Ning, Xuanxuan,Liu, Feng,Zhao, Jianmin,Wang, Qing,&Wu, Huifeng.(2013).The response profiles of HSPA12A and TCTP from Mytilus galloprovincialis to pathogen and cadmium challenge.FISH & SHELLFISH IMMUNOLOGY,35(2),343-350. |
| MLA | You, Liping,et al."The response profiles of HSPA12A and TCTP from Mytilus galloprovincialis to pathogen and cadmium challenge".FISH & SHELLFISH IMMUNOLOGY 35.2(2013):343-350. |
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