Uptake pathways and subcellular fractionation of Cd in the polychaete Nereis diversicolor | |
Li, Lianzhen1,2; Liu, Xiaoli1,2,3; You, Liping1,2,3; Zhang, Linbao1,2,3; Zhao, Jianmin1,2; Wu, Huifeng1,2 | |
发表期刊 | ECOTOXICOLOGY |
ISSN | 0963-9292 |
2012 | |
卷号 | 21期号:1页码:104-110 |
关键词 | Cd Uptake Mechanism Metal Accumulation Nereis Diversicolor |
产权排序 | [Li, Lianzhen; Liu, Xiaoli; You, Liping; Zhang, Linbao; Zhao, Jianmin; Wu, Huifeng] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Li, Lianzhen; Liu, Xiaoli; You, Liping; Zhang, Linbao; Zhao, Jianmin; Wu, Huifeng] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Liu, Xiaoli; You, Liping; Zhang, Linbao] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
通讯作者 | Wu, HF (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China. hfwu@yic.ac.cn |
作者部门 | 污染过程与控制实验室 |
英文摘要 | Polychaetes have often been utilized as indicator species to investigate the impacts of pollutants, such as heavy metals. The uptake of Cd by the polychaete Nereis diversicolor was determined at varying Ca concentrations and with pre-exposure to Ca ion channel blockers and metabolic inhibitors in simulated sea water over 1 week period. The supply of Ca in simulated sea water inhibited Cd uptake and increased Ca concentration in N. diversicolor after 10 mu M Cd exposure. Pre-exposure to a Ca-channel blocker (Lanthanum) significantly inhibited Cd uptake, suggesting that the uptake of Cd was exerted at a Ca channel. N-ethylmaleimide, which specifically binds to sulfhydryl groups, inhibited Cd uptake at 10 mu M, implying that the transport of Cd is carrier-mediated by proteins or other SH-containing compounds. Subcellular Cd distribution analysis showed that more than 60% of the total Cd associated with the cytosolic fraction. The presence of higher concentration of Ca in simulated sea water did not impact the proportional subcellular distribution of Cd in N. diversicolor. Nevertheless, the supply of Ca could significantly lower Cd concentration in cytosol and cellular debris. The present study provides evidence that Cd transport by N. diversicolor was mediated mainly through lanthanum- sensitive Ca ion channels and accumulated by SH-containing compounds. These results help to understand the uptake mechanism and subcellular distribution of Cd in polychaetes. |
文章类型 | Article |
资助机构 | Chinese Academy of Sciences[KZCX2-YW-223, KZCX2-YW-225]; SDSFC[ZR2009CZ008]; Key Laboratory of Pollution Processes and Environmental Criteria (Nankai University); Chinese Academy of Sciences, Ministry of Education[KL-PPEC-2010-6]; CAS/SAFEA |
收录类别 | SCI |
语种 | 英语 |
关键词[WOS] | SCALLOP CHLAMYS-NOBILIS ; CADMIUM UPTAKE ; MEMBRANE-VESICLES ; ZINC TRANSPORT ; PERNA-VIRIDIS ; TRACE-METALS ; CALCIUM ; INHIBITION ; EARTHWORMS ; MECHANISMS |
研究领域[WOS] | Environmental Sciences & Ecology ; Toxicology |
WOS记录号 | WOS:000298996900012 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.yic.ac.cn/handle/133337/5621 |
专题 | 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 中国科学院海岸带环境过程与生态修复重点实验室 |
作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Li, Lianzhen,Liu, Xiaoli,You, Liping,et al. Uptake pathways and subcellular fractionation of Cd in the polychaete Nereis diversicolor[J]. ECOTOXICOLOGY,2012,21(1):104-110. |
APA | Li, Lianzhen,Liu, Xiaoli,You, Liping,Zhang, Linbao,Zhao, Jianmin,&Wu, Huifeng.(2012).Uptake pathways and subcellular fractionation of Cd in the polychaete Nereis diversicolor.ECOTOXICOLOGY,21(1),104-110. |
MLA | Li, Lianzhen,et al."Uptake pathways and subcellular fractionation of Cd in the polychaete Nereis diversicolor".ECOTOXICOLOGY 21.1(2012):104-110. |
条目包含的文件 | 下载所有文件 | |||||
文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
Uptake pathways and (301KB) | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 下载 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论