Assessment of Clam Ruditapes philippinarum as Heavy Metal Bioindicators Using NMR-Based Metabolomics
Liu, Xiaoli1,2; Zhang, Linbao1,2; You, Liping1,2; Yu, Junbao1; Cong, Ming1; Wang, Qing1; Li, Fei1; Li, Lianzhen1; Zhao, Jianmin1; Li, Chenghua1; Wu, Huifeng1
Source PublicationCLEAN-SOIL AIR WATER
ISSN1863-0650
2011-08-01
Volume39Issue:8Pages:759-766
KeywordBioindicator Manila Clam Mercury Metabolomics Nmr
Contribution Rank[Liu, XL; Zhang, LB; You, LP; Yu, JB; Cong, M; Wang, Q; Li, F; Li, LZ; Zhao, JM; Li, CH; Wu, HF] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc,CAS, Yantai 264003, Peoples R China; [Liu, XL; Zhang, LB; You, LP] Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
Corresponding AuthorWu, HF, Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc,CAS, Yantai 264003, Peoples R China. hfwu@yic.ac.cn
Department污染过程与控制实验室 
AbstractThere are mainly distributed three pedigrees (White, Liangdao Red, and Zebra) of Manila clam Ruditapes philippinarum in Yantai population along the Bohai marine and coast. However, the biological differences to environmental stressors have been ignored in toxicology studies, which could lead to the distortion of biological interpretations of toxicological effects induced by environmental contaminants. In this study, we applied a system biology approach, metabolomics to compare the metabolic profiles in digestive gland from three pedigrees of clam and characterize and compare the metabolic responses induced by mercury in clam digestive gland tissues to determine a sensitive pedigree of clam as a preferable bioindicator for metal pollution monitoring and toxicology research. The most abundant metabolites, respectively, included branched-chain amino acids, alanine, and arginine in White samples, glutamate, dimethylglycine, and glycine in Zebra clams and acetylcholine, betaine, glucose, and glycogen in Liangdao Red clams. After 48 h exposure of 20 mu g L(-1) Hg(2+), the metabolic profiles from the three pedigrees of clams showed differentially significant changes in alanine, glutamate, succinate, taurine, hypotaurine, glycine, arginine, glucose, etc. Our findings indicate the toxicological effects of mercury exposure in Manila clams including the neurotoxicity, disturbances in energetic metabolisms and osmoregulation in the digestive glands and suggest that Liangdao Red pedigree of clam could be a preferable bioindicator for the metal pollution monitoring based on the more sensitive classes of metabolic changes from digestive glands compared with other two (White and Zebra) pedigrees of clams.; There are mainly distributed three pedigrees (White, Liangdao Red, and Zebra) of Manila clam Ruditapes philippinarum in Yantai population along the Bohai marine and coast. However, the biological differences to environmental stressors have been ignored in toxicology studies, which could lead to the distortion of biological interpretations of toxicological effects induced by environmental contaminants. In this study, we applied a system biology approach, metabolomics to compare the metabolic profiles in digestive gland from three pedigrees of clam and characterize and compare the metabolic responses induced by mercury in clam digestive gland tissues to determine a sensitive pedigree of clam as a preferable bioindicator for metal pollution monitoring and toxicology research. The most abundant metabolites, respectively, included branched-chain amino acids, alanine, and arginine in White samples, glutamate, dimethylglycine, and glycine in Zebra clams and acetylcholine, betaine, glucose, and glycogen in Liangdao Red clams. After 48 h exposure of 20 mu g L-1 Hg2+, the metabolic profiles from the three pedigrees of clams showed differentially significant changes in alanine, glutamate, succinate, taurine, hypotaurine, glycine, arginine, glucose, etc. Our findings indicate the toxicological effects of mercury exposure in Manila clams including the neurotoxicity, disturbances in energetic metabolisms and osmoregulation in the digestive glands and suggest that Liangdao Red pedigree of clam could be a preferable bioindicator for the metal pollution monitoring based on the more sensitive classes of metabolic changes from digestive glands compared with other two (White and Zebra) pedigrees of clams.
SubtypeArticle
Indexed BySCI
Language英语
WOS KeywordLAGOON-OF-VENICE ; TAPES-PHILIPPINARUM ; MYTILUS-GALLOPROVINCIALIS ; H-1-NMR METABOLOMICS ; PATTERN-RECOGNITION ; MANILA CLAM ; SPECTROSCOPY ; METABONOMICS ; EXTRACTION ; POLLUTION
WOS Research AreaEnvironmental Sciences & Ecology ; Marine & Freshwater Biology ; Water Resources
WOS IDWOS:000294720700007
Citation statistics
Cited Times:23[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/5117
Collection中科院海岸带环境过程与生态修复重点实验室_污染过程与控制实验室
中科院海岸带环境过程与生态修复重点实验室
Affiliation1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc,CAS, Yantai 264003, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Liu, Xiaoli,Zhang, Linbao,You, Liping,et al. Assessment of Clam Ruditapes philippinarum as Heavy Metal Bioindicators Using NMR-Based Metabolomics[J]. CLEAN-SOIL AIR WATER,2011,39(8):759-766.
APA Liu, Xiaoli.,Zhang, Linbao.,You, Liping.,Yu, Junbao.,Cong, Ming.,...&Wu, Huifeng.(2011).Assessment of Clam Ruditapes philippinarum as Heavy Metal Bioindicators Using NMR-Based Metabolomics.CLEAN-SOIL AIR WATER,39(8),759-766.
MLA Liu, Xiaoli,et al."Assessment of Clam Ruditapes philippinarum as Heavy Metal Bioindicators Using NMR-Based Metabolomics".CLEAN-SOIL AIR WATER 39.8(2011):759-766.
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