Characterization of a marine-isolated mercury-resistant Pseudomonas putida strain SP1 and its potential application in marine mercury reduction
Zhang, Weiwei1,2; Chen, Lingxin1,2; Liu, Dongyan1,2
2012-02-01
Source PublicationAPPLIED MICROBIOLOGY AND BIOTECHNOLOGY
ISSN0175-7598
Volume93Issue:3Pages:1305-1314
Contribution Rank(1)Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, 17 Chunhui Road, Yantai, 264003, China; (2)Key Laboratory of Coastal Zone Environmental Processes, Chinese Academy of Sciences; Shandong Provin, Chinese Academy of Sciences, Yantai, China
AbstractThe Pseudomonas putida strain SP1 was isolated from marine environment and was found to be resist © 2011 Springer-Verlag.; The Pseudomonas putida strain SP1 was isolated from marine environment and was found to be resistant to 280 mu M HgCl2. SP1 was also highly resistant to other metals, including CdCl2, CoCl2, CrCl3, CuCl2, PbCl2, and ZnSO4, and the antibiotics ampicillin (Ap), kanamycin (Kn), chloramphenicol (Cm), and tetracycline (Tc). mer operon, possessed by most mercury-resistant bacteria, and other diverse types of resistant determinants were all located on the bacterial chromosome. Cold vapor atomic absorption spectrometry and a volatilization test indicated that the isolated P. putida SP1 was able to volatilize almost 100% of the total mercury it was exposed to and could potentially be used for bioremediation in marine environments. The optimal pH for the growth of P. putida SP1 in the presence of HgCl2 and the removal of HgCl2 by P. putida SP1 was between 8.0 and 9.0, whereas the optimal pH for the expression of merA, the mercuric reductase enzyme in mer operon that reduces reactive Hg2+ to volatile and relatively inert monoatomic Hg-0 vapor, was around 5.0. LD50 of P. putida SP1 to flounder and turbot was 1.5x 10(9) CFU. Biofilm developed by P. putida SP1 was 1- to 3-fold lower than biofilm developed by an aquatic pathogen Pseudomonas fluorescens TSS. The results of this study indicate that P. putida SP1 is a low virulence strain that can potentially be applied in the bioremediation of HgCl2 contamination over a broad range of pH.
KeywordPseudomonas Putida Marine Environment Mer Operon Bioremediation Of Hgcl2 Contamination
Department环境化学实验室
URL查看原文
Indexed BySCI
WOS KeywordBACTERIA HIGHLY RESISTANT ; ESCHERICHIA-COLI ; MOLECULAR APPLICATION ; WASTE-WATER ; MER OPERON ; TRANSPOSONS ; BIOREMEDIATION ; TEMPERATURE ; SEQUENCES ; VIRULENCE
SubtypeArticle
Language英语
WOS Research AreaBiotechnology & Applied Microbiology
WOS IDWOS:000300282500038
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Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/4940
Collection中科院海岸带环境过程与生态修复重点实验室_环境化学实验室
中科院海岸带环境过程与生态修复重点实验室
Affiliation1.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai, Peoples R China
2.Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai, Peoples R China
First Author AffilicationYantai Institute of Coastal Zone Research, Chinese Academy of Sciences
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Zhang, Weiwei,Chen, Lingxin,Liu, Dongyan. Characterization of a marine-isolated mercury-resistant Pseudomonas putida strain SP1 and its potential application in marine mercury reduction[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2012,93(3):1305-1314.
APA Zhang, Weiwei,Chen, Lingxin,&Liu, Dongyan.(2012).Characterization of a marine-isolated mercury-resistant Pseudomonas putida strain SP1 and its potential application in marine mercury reduction.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,93(3),1305-1314.
MLA Zhang, Weiwei,et al."Characterization of a marine-isolated mercury-resistant Pseudomonas putida strain SP1 and its potential application in marine mercury reduction".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 93.3(2012):1305-1314.
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