| Characterization of a marine-isolated mercury-resistant Pseudomonas putida strain SP1 and its potential application in marine mercury reduction | |
Zhang, Weiwei1,2; Chen, Lingxin1,2 ; Liu, Dongyan1,2
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| 发表期刊 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
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| ISSN | 0175-7598 |
| 2012-02-01 | |
| 卷号 | 93期号:3页码:1305-1314 |
| 关键词 | Pseudomonas Putida Marine Environment Mer Operon Bioremediation Of Hgcl2 Contamination |
| 产权排序 | (1)Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, 17 Chunhui Road, Yantai, 264003, China; (2)Key Laboratory of Coastal Zone Environmental Processes, Chinese Academy of Sciences; Shandong Provin, Chinese Academy of Sciences, Yantai, China |
| 作者部门 | 环境化学实验室 |
| 英文摘要 | The Pseudomonas putida strain SP1 was isolated from marine environment and was found to be resist © 2011 Springer-Verlag.; The Pseudomonas putida strain SP1 was isolated from marine environment and was found to be resistant to 280 mu M HgCl2. SP1 was also highly resistant to other metals, including CdCl2, CoCl2, CrCl3, CuCl2, PbCl2, and ZnSO4, and the antibiotics ampicillin (Ap), kanamycin (Kn), chloramphenicol (Cm), and tetracycline (Tc). mer operon, possessed by most mercury-resistant bacteria, and other diverse types of resistant determinants were all located on the bacterial chromosome. Cold vapor atomic absorption spectrometry and a volatilization test indicated that the isolated P. putida SP1 was able to volatilize almost 100% of the total mercury it was exposed to and could potentially be used for bioremediation in marine environments. The optimal pH for the growth of P. putida SP1 in the presence of HgCl2 and the removal of HgCl2 by P. putida SP1 was between 8.0 and 9.0, whereas the optimal pH for the expression of merA, the mercuric reductase enzyme in mer operon that reduces reactive Hg2+ to volatile and relatively inert monoatomic Hg-0 vapor, was around 5.0. LD50 of P. putida SP1 to flounder and turbot was 1.5x 10(9) CFU. Biofilm developed by P. putida SP1 was 1- to 3-fold lower than biofilm developed by an aquatic pathogen Pseudomonas fluorescens TSS. The results of this study indicate that P. putida SP1 is a low virulence strain that can potentially be applied in the bioremediation of HgCl2 contamination over a broad range of pH. |
| 文章类型 | Article |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | BACTERIA HIGHLY RESISTANT ; ESCHERICHIA-COLI ; MOLECULAR APPLICATION ; WASTE-WATER ; MER OPERON ; TRANSPOSONS ; BIOREMEDIATION ; TEMPERATURE ; SEQUENCES ; VIRULENCE |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| WOS记录号 | WOS:000300282500038 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/4940 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室 中国科学院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai, Peoples R China 2.Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Weiwei,Chen, Lingxin,Liu, Dongyan. Characterization of a marine-isolated mercury-resistant Pseudomonas putida strain SP1 and its potential application in marine mercury reduction[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2012,93(3):1305-1314. |
| APA | Zhang, Weiwei,Chen, Lingxin,&Liu, Dongyan.(2012).Characterization of a marine-isolated mercury-resistant Pseudomonas putida strain SP1 and its potential application in marine mercury reduction.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,93(3),1305-1314. |
| MLA | Zhang, Weiwei,et al."Characterization of a marine-isolated mercury-resistant Pseudomonas putida strain SP1 and its potential application in marine mercury reduction".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 93.3(2012):1305-1314. |
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