| Differential toxicological effects induced by mercury in gills from three pedigrees of Manila clam Ruditapes philippinarum by NMR-based metabolomics | |
Liu, Xiaoli1,2; Zhang, Linbao1,2; You, Liping1,2; Yu, Junbao1 ; Zhao, Jianmin1; Li, Lianzhen1; Wang, Qing1; Li, Fei1; Li, Chenghua1; Liu, Dongyan1; Wu, Huifeng1
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| 发表期刊 | ECOTOXICOLOGY
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| 2011 | |
| 卷号 | 20期号:1页码:177-186 |
| 关键词 | Manila Clam Bioindicator Mercury Nmr Metabolomics |
| 产权排序 | [Liu, Xiaoli; Zhang, Linbao; You, Liping; Yu, Junbao; Zhao, Jianmin; Li, Lianzhen; Wang, Qing; Li, Fei; Li, Chenghua; Liu, Dongyan; Wu, Huifeng] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res,CAS, Yantai 264003, Peoples R China; [Liu, Xiaoli; Zhang, Linbao; You, Liping] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 通讯作者 | Wu, HF, Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res,CAS, Yantai 264003, Peoples R China.hfwu@yic.ac.cn |
| 作者部门 | 污染过程与控制实验室 |
| 英文摘要 | Mercury is a hazardous pollutant in the Bohai marine environments due to its high toxicity to the marine organisms and subsequent ecological risk. Manila clam Ruditapes philippinarum is one of important sentinel organisms in 'Mussel Watch Program' launched in China and therefore used as a bioindicator in marine and coastal ecotoxicology. There are dominantly distributed three pedigrees of clam (White, Liangdao Red and Zebra) in Yantai population endowed with different tolerances to environmental stressors. In this study, gill tissues were collected from both untreated and mercury exposed White, Liangdao Red and Zebra clams, and the extracts were analyzed by NMR-based metabolomics to compare the original metabolomes and the toxicological effects induced by mercury exposure in three pedigrees. The major abundant metabolites in White clam sample were branched-chain amino acids, lactate, alanine, arginine, acetoacetate, glutamate, succinate, citrate, malonate and taurine, while the metabolite profile of Liangdao Red clam sample comprises relative high levels of alanine, arginine, glutamate, succinate and glycogen. For Zebra clam sample, the metabolite profile exhibited relatively high amount of aspartate, acetylcholine and homarine. After 48 h exposure of 20 mu g l(-1) Hg2+, the metabolic profiles from all the three pedigrees of clams commonly showed significant increases in alanine, arginine, glutamate, aspartate, alpha-ketoglutarate, glycine and ATP/ADP, and decreases in citrate, taurine and homarine. The unique metabolic differences between the metabolomes of gill tissues from Hg2+-exposed White, Liangdao Red and Zebra clams were found, including elevated acetylcholine and branched-chain amino acids in White clams, and the declined succinate in both White and Liangdao Red samples as well as the declined betaine in Zebra and White clams. Overall, our findings showed the differential toxicological responses to mercury exposure and that White clams could be a preferable bioindicator for the metal pollution monitoring based on the metabolic changes from gill compared with other two (Liangdao Red and Zebra) pedigrees of clams.; Mercury is a hazardous pollutant in the Bohai marine environments due to its high toxicity to the marine organisms and subsequent ecological risk. Manila clam Ruditapes philippinarum is one of important sentinel organisms in 'Mussel Watch Program' launched in China and therefore used as a bioindicator in marine and coastal ecotoxicology. There are dominantly distributed three pedigrees of clam (White, Liangdao Red and Zebra) in Yantai population endowed with different tolerances to environmental stressors. In this study, gill tissues were collected from both untreated and mercury exposed White, Liangdao Red and Zebra clams, and the extracts were analyzed by NMR-based metabolomics to compare the original metabolomes and the toxicological effects induced by mercury exposure in three pedigrees. The major abundant metabolites in White clam sample were branched-chain amino acids, lactate, alanine, arginine, acetoacetate, glutamate, succinate, citrate, malonate and taurine, while the metabolite profile of Liangdao Red clam sample comprises relative high levels of alanine, arginine, glutamate, succinate and glycogen. For Zebra clam sample, the metabolite profile exhibited relatively high amount of aspartate, acetylcholine and homarine. After 48 h exposure of 20 mu g l(-1) Hg2+, the metabolic profiles from all the three pedigrees of clams commonly showed significant increases in alanine, arginine, glutamate, aspartate, alpha-ketoglutarate, glycine and ATP/ADP, and decreases in citrate, taurine and homarine. The unique metabolic differences between the metabolomes of gill tissues from Hg2+-exposed White, Liangdao Red and Zebra clams were found, including elevated acetylcholine and branched-chain amino acids in White clams, and the declined succinate in both White and Liangdao Red samples as well as the declined betaine in Zebra and White clams. Overall, our findings showed the differential toxicological responses to mercury exposure and that White clams could be a preferable bioindicator for the metal pollution monitoring based on the metabolic changes from gill compared with other two (Liangdao Red and Zebra) pedigrees of clams. |
| 文章类型 | Article |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | H-1-NMR METABOLOMICS ; PATTERN-RECOGNITION ; OXIDATIVE STRESS ; TIDAL FLATS ; SPECTROSCOPY ; METABONOMICS ; EXTRACTION ; ALANINE ; TISSUE ; LA(NO3)(3) |
| 研究领域[WOS] | Environmental Sciences & Ecology ; Toxicology |
| WOS记录号 | WOS:000286003700019 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/4889 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_污染过程与控制实验室 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室 中国科学院海岸带环境过程与生态修复重点实验室_滨海湿地实验室 中国科学院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res,CAS, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Xiaoli,Zhang, Linbao,You, Liping,et al. Differential toxicological effects induced by mercury in gills from three pedigrees of Manila clam Ruditapes philippinarum by NMR-based metabolomics[J]. ECOTOXICOLOGY,2011,20(1):177-186. |
| APA | Liu, Xiaoli.,Zhang, Linbao.,You, Liping.,Yu, Junbao.,Zhao, Jianmin.,...&Wu, Huifeng.(2011).Differential toxicological effects induced by mercury in gills from three pedigrees of Manila clam Ruditapes philippinarum by NMR-based metabolomics.ECOTOXICOLOGY,20(1),177-186. |
| MLA | Liu, Xiaoli,et al."Differential toxicological effects induced by mercury in gills from three pedigrees of Manila clam Ruditapes philippinarum by NMR-based metabolomics".ECOTOXICOLOGY 20.1(2011):177-186. |
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