Rational design of a nitroreductase-activatable two-photon fluorescent probe for hypoxia imaging in cell and in vivo
Wang, Yue; Zhang, Li; Huang, Yan; Wang, Xiaoyan; Zhang, Liangwei; Chen, Lingxin
Source PublicationSENSORS AND ACTUATORS B-CHEMICAL
2020-05-01
Volume310Pages:127755
KeywordMOLECULAR-MECHANISMS SELECTIVE DETECTION LUNG-CANCER OXYGEN ZEBRAFISH CISPLATIN APOPTOSIS TUMORS
MOST Discipline CatalogueChemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
DOI10.1016/j.snb.2020.127755
Contribution Rank[Wang, Yue; Zhang, Li; Huang, Yan; Zhang, Liangwei; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Huang, Yan; Wang, Xiaoyan; Chen, Lingxin] Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China; [Chen, Lingxin] Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China; [Wang, Yue; Zhang, Li] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Department海岸带环境工程技术研究与发展中心
AbstractTumor resistance is a huge challenge for tumor treatment, which may lead to tumor treatment failure. Relieving tumor resistance and improving efficacy are long-term goals for tumor treatment. Nitroreductase (NTR) is an endogenous enzyme that highly expressed in hypoxia tumors. Herein, we develop a NTR-activatable fluorescent probe, TP-NO2, for NTR detection during tumor treatment. TP-NO2 with simple synthesis steps and high yield can qualitatively and quantitatively detect NTR in a wide range (0 - 20 mu g/mL) with a detection limit of 26 ng/mL. The probe has been successfully applied for NTR detection in cells under simulated hypoxia conditions. Hyperbaric oxygen (HBO) can alleviate tumor hypoxia and reduce NTR concentration. We pre-evaluate the effect of tumor treatment by NTR imaging. And the results demonstrate that HBO-assistant chemotherapy can effectively treat tumor cells. We further apply TP-NO2 for NTR detection in A549 and cis-dichlorodiamineplatinum(II) (DDP)-resistant A549 (A549/DDP) xenograft nude mice, and we choose NTR as an indicator to pre-evaluate the treatment efficacy of malignant tumors. With the adjuvant therapy of HBO, chemotherapeutic drugs gemcitabine and carboplatin can effectively treat A549 and A549/DDP xenograft. These applications provide us a novel perspective for NTR imaging.
SubtypeArticle
Indexed BySCI
Language英语
WOS KeywordMOLECULAR-MECHANISMS ; SELECTIVE DETECTION ; LUNG-CANCER ; OXYGEN ; ZEBRAFISH ; CISPLATIN ; APOPTOSIS ; TUMORS
WOS IDWOS:000519306300046
Funding OrganizationNational Nature Science Foundation of ChinaNational Natural Science Foundation of China [21976209, 21778026] ; program of Youth Innovation Promotion Association, CAS [2019217] ; Taishan Scholar Project Special Fund [ts20190962]
Citation statistics
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/25042
Collection中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
Affiliation1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China;
2.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China;
3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China;
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Wang, Yue,Zhang, Li,Huang, Yan,et al. Rational design of a nitroreductase-activatable two-photon fluorescent probe for hypoxia imaging in cell and in vivo[J]. SENSORS AND ACTUATORS B-CHEMICAL,2020,310:127755.
APA Wang, Yue,Zhang, Li,Huang, Yan,Wang, Xiaoyan,Zhang, Liangwei,&Chen, Lingxin.(2020).Rational design of a nitroreductase-activatable two-photon fluorescent probe for hypoxia imaging in cell and in vivo.SENSORS AND ACTUATORS B-CHEMICAL,310,127755.
MLA Wang, Yue,et al."Rational design of a nitroreductase-activatable two-photon fluorescent probe for hypoxia imaging in cell and in vivo".SENSORS AND ACTUATORS B-CHEMICAL 310(2020):127755.
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