| Rational design of a nitroreductase-activatable two-photon fluorescent probe for hypoxia imaging in cell and in vivo | |
Wang, Yue; Zhang, Li; Huang, Yan; Wang, Xiaoyan; Zhang, Liangwei ; Chen, Lingxin
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| Source Publication | SENSORS AND ACTUATORS B-CHEMICAL
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| 2020-05-01 | |
| Volume | 310Pages:127755 |
| Keyword | MOLECULAR-MECHANISMS SELECTIVE DETECTION LUNG-CANCER OXYGEN ZEBRAFISH CISPLATIN APOPTOSIS TUMORS |
| MOST Discipline Catalogue | Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation |
| DOI | 10.1016/j.snb.2020.127755 |
| Contribution Rank | [Wang, Yue; Zhang, Li; Huang, Yan; Zhang, Liangwei; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Huang, Yan; Wang, Xiaoyan; Chen, Lingxin] Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China; [Chen, Lingxin] Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China; [Wang, Yue; Zhang, Li] Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| Department | 海岸带环境工程技术研究与发展中心 |
| Abstract | Tumor resistance is a huge challenge for tumor treatment, which may lead to tumor treatment failure. Relieving tumor resistance and improving efficacy are long-term goals for tumor treatment. Nitroreductase (NTR) is an endogenous enzyme that highly expressed in hypoxia tumors. Herein, we develop a NTR-activatable fluorescent probe, TP-NO2, for NTR detection during tumor treatment. TP-NO2 with simple synthesis steps and high yield can qualitatively and quantitatively detect NTR in a wide range (0 - 20 mu g/mL) with a detection limit of 26 ng/mL. The probe has been successfully applied for NTR detection in cells under simulated hypoxia conditions. Hyperbaric oxygen (HBO) can alleviate tumor hypoxia and reduce NTR concentration. We pre-evaluate the effect of tumor treatment by NTR imaging. And the results demonstrate that HBO-assistant chemotherapy can effectively treat tumor cells. We further apply TP-NO2 for NTR detection in A549 and cis-dichlorodiamineplatinum(II) (DDP)-resistant A549 (A549/DDP) xenograft nude mice, and we choose NTR as an indicator to pre-evaluate the treatment efficacy of malignant tumors. With the adjuvant therapy of HBO, chemotherapeutic drugs gemcitabine and carboplatin can effectively treat A549 and A549/DDP xenograft. These applications provide us a novel perspective for NTR imaging. |
| Subtype | Article |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | MOLECULAR-MECHANISMS ; SELECTIVE DETECTION ; LUNG-CANCER ; OXYGEN ; ZEBRAFISH ; CISPLATIN ; APOPTOSIS ; TUMORS |
| WOS ID | WOS:000519306300046 |
| Funding Organization | National Nature Science Foundation of ChinaNational Natural Science Foundation of China [21976209, 21778026] ; program of Youth Innovation Promotion Association, CAS [2019217] ; Taishan Scholar Project Special Fund [ts20190962] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.yic.ac.cn/handle/133337/25042 |
| Collection | 中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心 |
| Affiliation | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; 2.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China; 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China; 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| Recommended Citation GB/T 7714 | Wang, Yue,Zhang, Li,Huang, Yan,et al. Rational design of a nitroreductase-activatable two-photon fluorescent probe for hypoxia imaging in cell and in vivo[J]. SENSORS AND ACTUATORS B-CHEMICAL,2020,310:127755. |
| APA | Wang, Yue,Zhang, Li,Huang, Yan,Wang, Xiaoyan,Zhang, Liangwei,&Chen, Lingxin.(2020).Rational design of a nitroreductase-activatable two-photon fluorescent probe for hypoxia imaging in cell and in vivo.SENSORS AND ACTUATORS B-CHEMICAL,310,127755. |
| MLA | Wang, Yue,et al."Rational design of a nitroreductase-activatable two-photon fluorescent probe for hypoxia imaging in cell and in vivo".SENSORS AND ACTUATORS B-CHEMICAL 310(2020):127755. |
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