Molecular cloning and characterization of FADD from the manila clam Ruditapes philippinarum

doi:10.1016/j.fsi.2019.03.033

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	Molecular cloning and characterization of FADD from the manila clam Ruditapes philippinarum
	Hu, GG ; Han, YJ ; Yang, DL; Cao, RW ; Wang, Q; Liu, H; Dong, ZJ ; Zhang, XL; Zhang, QQ; Zhao, JM
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
	2019-05
卷号	88 页码:556-566
关键词	FADD Ruditapes philippinarum NF-kappa B Apoptosis
研究领域	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI	10.1016/j.fsi.2019.03.033
产权排序	[Hu, Gege; Han, Yijing; Yang, Dinglong; Cao, Ruiwen; Wang, Qing; Liu, Hui; Dong, Zhijun; Zhang, Xiaoli; Zhang, Qianqian; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China; [Yang, Dinglong; Wang, Qing; Liu, Hui; Dong, Zhijun; Zhang, Xiaoli; Zhang, Qianqian; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai 264003, Peoples R China; [Zhao, Jianmin] Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China; [Hu, Gege; Han, Yijing; Cao, Ruiwen] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
通讯作者	Yang, Dinglong(dlyang@yic.ac.cn) ; Zhao, Jianmin(jmzhao@yic.ac.cn)
作者部门	海岸带生物资源高效利用研究与发展中心
英文摘要	Fas-associated protein with death domain (FADD) is an essential element in cell death, and also implicates in cell cycle progression, inflammation and innate immunity. In the study, an FADD (designated as RpFADD) was identified and characterized from manila clam, Ruditapes philippinarum. Multiple alignments and phylogenetic analysis strongly suggested that RpFADD was a new member of the FADD family. The RpFADD transcripts were constitutively expressed in a wide range of tissues, and dominantly expressed in hemocytes. After challenged with Vibrio anguillarum or Micrococcus luteus, the expression level of RpFADD transcripts was significantly induced and reached the maximum level at 72 h and 48 h, respectively. Knockdown of RpFADD down-regulated the transcript levels of Rp1KK, RpTAK1 and RpNF-kappa B with the exception of RpI kappa B. Moreover, RpFADD primarily localized in the cell cytoplasm, and its over-expression promoted the apoptosis of HeLa cells. These results revealed that RpFADD perhaps regulated the NF-kappa B signaling pathways positively, which provided a better understanding of RpFADD in innate immunity.
文章类型	Article
资助机构	Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDA23050303] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [41806196] ; Youth Innovation Promotion Association CAS [2016196]
收录类别	SCI
语种	英语
关键词[WOS]	NF-KAPPA-B ; DEATH DOMAIN ; EXPRESSION ; PROTEIN ; FAS ; ASSOCIATION ; ACTIVATION ; FLICE
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:000465060500065
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/24949
专题	海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室中国科学院海岸带环境过程与生态修复重点实验室支撑部门_中国科学院牟平海岸带环境综合试验站
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China; 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai 264003, Peoples R China; 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China; 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Hu, GG,Han, YJ,Yang, DL,et al. Molecular cloning and characterization of FADD from the manila clam Ruditapes philippinarum[J]. FISH & SHELLFISH IMMUNOLOGY,2019,88:556-566.
APA	Hu, GG.,Han, YJ.,Yang, DL.,Cao, RW.,Wang, Q.,...&Zhao, JM.(2019).Molecular cloning and characterization of FADD from the manila clam Ruditapes philippinarum.FISH & SHELLFISH IMMUNOLOGY,88,556-566.
MLA	Hu, GG,et al."Molecular cloning and characterization of FADD from the manila clam Ruditapes philippinarum".FISH & SHELLFISH IMMUNOLOGY 88(2019):556-566.

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