| High Spatiotemporal Resolution Observation of Glutathione Hydropersulfides in Living Cells and Tissue via a Two-Photon Ratiometric Fluorescent Probe | |
Wang, Y ; Zhang, LW; Zhang, SZ; Liu, Z; Chen, LX
| |
| 发表期刊 | ANALYTICAL CHEMISTRY
 |
| ISSN | 0003-2700 |
| 2019-06-18 | |
| 卷号 | 91期号:12页码:7812-7818 |
| 关键词 | MITOCHONDRIAL AUTOPHAGY OXIDATIVE STRESS HYDROGEN-SULFIDE SYNTHASE CBS MERCURY PERSULFIDES CYSTEINE SULFUR DISULFIDE APOPTOSIS |
| 研究领域 | Chemistry, Analytical |
| DOI | 10.1021/acs.analchem.9b01511 |
| 产权排序 | [Wang, Yue; Zhang, Liangwei; Zhang, Songzi; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Re, Yantai 264003, Peoples R China; [Chen, Lingxin] Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China; [Liu, Zhe; Chen, Lingxin] Qufu Normal Univ, Dept Chem & Chem Engn, Qufu 273165, Peoples R China; [Chen, Lingxin] Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China; [Wang, Yue] Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 通讯作者 | Chen, Lingxin(lxchen@yic.ac.cn) |
| 作者部门 | 海岸带环境工程技术研究与发展中心 |
| 英文摘要 | Glutathione hydropersulfides (GSSH) are alluded to play crucial roles in signal transduction, redox homeostasis, and metabolic regulation. However, the detailed biological functions of GSSH in these aspects are extremely ambiguous. The key barrier to understand the role of GSSH in biological systems is a lack of detection tools with high spatiotemporal resolution. To address the issues, we are seeking novel chemical tools for GSSH detection. We herein develop the first two-photon ratiometric fluorescent probe (TP-Dise) for GSSH detection with high spatial and temporal resolution in living cells and tissue. On the basis of our probe TP-Dise, we investigate the biosynthesis of GSSH, and the results indicate that GSSH is mainly from two sulfurtransferases, CBS and CSE. Furthermore, we explore the biological function of GSSH in protecting cells from mercury ion-induced cell damage for the first time. The experimental results indicate that mercury ions may induce cell death by causing mitochondrial autophagy. GSSH acts both as antagonist and as antioxidant and can effectively alleviate the damage caused by mercury stress. |
| 文章类型 | Article |
| 资助机构 | National Nature Science Foundation of ChinaNational Natural Science Foundation of China [21575159, 21778026] ; program of Youth Innovation Promotion Association, CAS [2019217] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | MITOCHONDRIAL AUTOPHAGY ; OXIDATIVE STRESS ; HYDROGEN-SULFIDE ; SYNTHASE CBS ; MERCURY ; PERSULFIDES ; CYSTEINE ; SULFUR ; DISULFIDE ; APOPTOSIS |
| 研究领域[WOS] | Chemistry, Analytical |
| WOS记录号 | WOS:000472682000042 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/24919 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 中国科学院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Re, Yantai 264003, Peoples R China; 2.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China; 3.Qufu Normal Univ, Dept Chem & Chem Engn, Qufu 273165, Peoples R China; 4.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China; 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Y,Zhang, LW,Zhang, SZ,et al. High Spatiotemporal Resolution Observation of Glutathione Hydropersulfides in Living Cells and Tissue via a Two-Photon Ratiometric Fluorescent Probe[J]. ANALYTICAL CHEMISTRY,2019,91(12):7812-7818. |
| APA | Wang, Y,Zhang, LW,Zhang, SZ,Liu, Z,&Chen, LX.(2019).High Spatiotemporal Resolution Observation of Glutathione Hydropersulfides in Living Cells and Tissue via a Two-Photon Ratiometric Fluorescent Probe.ANALYTICAL CHEMISTRY,91(12),7812-7818. |
| MLA | Wang, Y,et al."High Spatiotemporal Resolution Observation of Glutathione Hydropersulfides in Living Cells and Tissue via a Two-Photon Ratiometric Fluorescent Probe".ANALYTICAL CHEMISTRY 91.12(2019):7812-7818. |
| 条目包含的文件 | 下载所有文件 | |||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| High Spatiotemporal （2883KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 下载 | |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论