Evaluation of mitochondrial toxicity of cadmium in clam Ruditapes philippinarum using iTRAQ-based proteomics

doi:10.1016/j.envpol.2019.05.046

	Evaluation of mitochondrial toxicity of cadmium in clam Ruditapes philippinarum using iTRAQ-based proteomics
	Ji, CL; Lu, Z ; Xu, LL ; Li, F; Cong, M; Shan, XJ ; Wu, HF
发表期刊	ENVIRONMENTAL POLLUTION
ISSN	0269-7491
	2019-08
卷号	251 页码:802-810
关键词	Cadmium iTRAQ Proteomics Mitochondrial toxicity Ruditapes philippinarum
研究领域	Environmental Sciences
DOI	10.1016/j.envpol.2019.05.046
产权排序	[Ji, Chenglong; Lu, Zhen; Xu, Lanlan; Li, Fei; Cong, Ming; Wu, Huifeng] Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res YIC, Shandong Key Lab Coastal Environm Proc,YICCAS, Yantai 264003, Peoples R China; [Ji, Chenglong; Shan, Xiujuan; Wu, Huifeng] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Shandong, Peoples R China; [Lu, Zhen; Xu, Lanlan] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
通讯作者	Wu, Huifeng(hfwu@yic.ac.cn)
作者部门	海岸带环境过程实验室
英文摘要	Cadmium is one of the most serious metal pollutants in the Bohai Sea. Previous studies revealed that mitochondrion might be the target organelle of Cd toxicity. However, there is a lack of a global view on the mitochondrial responses in marine animals to Cd. In this work, the mitochondrial responses were characterized in clams Ruditapes philippinarum treated with two concentrations (5 and 50 mu g/L) of Cd for 5 weeks using tetraethylbenzimidazolylcarbocyanine iodide (JC-1) staining, ultrastructural observation and quantitative proteomic analysis. Basically, a significant decrease of mitochondrial membrane potential (Delta Psi m) was observed in clams treated with the high concentration (50 mu g/L) of Cd. Cd treatments also induced specific morphological changes indicated by elongated mitochondria. Furthermore, iTRAQ-based mitochondrial proteomics showed that a total of 97 proteins were significantly altered in response to Cd treatment. These proteins were closely associated with multiple biological processes in mitochondria, including tricarboxylic acid (TCA) cycle, oxidative phosphorylation, fatty acid beta-oxidation, stress resistance and apoptosis, and mitochondrial fission. These findings confirmed that mitochondrion was one of the key targets of Cd toxicity. Moreover, dynamical regulations, such as reconstruction of energy homeostasis, induction of stress resistance and apoptosis, and morphological alterations, in mitochondria might play essential roles in Cd tolerance. Overall, this work provided a deep insight into the mitochondrial toxicity of Cd in clams based on a global mitochondrial proteomic analysis. (C) 2019 Elsevier Ltd. All rights reserved.
文章类型	Article
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China [41676114] ; Young Taishan Scholars Program of Shandong Province [tsqn201812115] ; Qingdao National Laboratory for Marine Science and Technology [QNLM201701] ; Youth Innovation Promotion Association CAS [2015169]
收录类别	SCI
语种	英语
关键词[WOS]	MUSSELS MYTILUS-GALLOPROVINCIALIS ; VIRGINICA GMELIN BIVALVIA ; SHRIMP CRANGON-AFFINIS ; METABOLIC-RESPONSES ; OXIDATIVE STRESS ; METAL POLLUTION ; MEMBRANE ; INSIGHTS ; FISSION ; BOHAI
研究领域[WOS]	Environmental Sciences
WOS记录号	WOS:000474329700088
引用统计	被引频次：30[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/24896
专题	中国科学院海岸带环境过程与生态修复重点实验室中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室
作者单位	1.Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res YIC, Shandong Key Lab Coastal Environm Proc,YICCAS, Yantai 264003, Peoples R China; 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Shandong, Peoples R China; 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Ji, CL,Lu, Z,Xu, LL,et al. Evaluation of mitochondrial toxicity of cadmium in clam Ruditapes philippinarum using iTRAQ-based proteomics[J]. ENVIRONMENTAL POLLUTION,2019,251:802-810.
APA	Ji, CL.,Lu, Z.,Xu, LL.,Li, F.,Cong, M.,...&Wu, HF.(2019).Evaluation of mitochondrial toxicity of cadmium in clam Ruditapes philippinarum using iTRAQ-based proteomics.ENVIRONMENTAL POLLUTION,251,802-810.
MLA	Ji, CL,et al."Evaluation of mitochondrial toxicity of cadmium in clam Ruditapes philippinarum using iTRAQ-based proteomics".ENVIRONMENTAL POLLUTION 251(2019):802-810.

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