Tissue distribution and functional characterization of mytimacin-4 in Mytilus galloprovincialis
Lv, JS; Zhao, JM; Yang, DL; Wu, HF; Cong, M
Source PublicationJOURNAL OF INVERTEBRATE PATHOLOGY
ISSN0022-2011
2019-09
Volume166Pages:107215
KeywordMytilus galloprovincialis Mytimacin-4 Tissue distribution Recombinant proteins Antimicrobial activity
MOST Discipline CatalogueZoology
DOI10.1016/j.jip.2019.107215
Contribution Rank[Lv, Jiasen] Yantai Univ, Biol Sch, Yantai 264005, Peoples R China; [Zhao, Jianmin; Yang, Dinglong; Wu, Huifeng; Cong, Ming] Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res YIC, Shandong Key Lab Coastal Environm Proc,YICCAS, Yantai 264003, Shandong, Peoples R China
Department海岸带环境过程实验室
AbstractAntimicrobial peptides (AMPs) play fundamental roles in the innate immunity of invertebrates. Mytimacin-4 is a kind of AMP gene previously sequenced from Mytilus galloprovincialis based on an identified EST sequence in our lab. In the present study, the tissue distribution and antimicrobial activities of mytimacin-4 were further investigated. A qRT-PCR analysis revealed that mytimacin-4 transcripts were constitutively expressed in all of the tested tissues of M. galloprovincialis, with the highest expression level in the posterior adductor muscle. After challenge by Vibrio anguillarum, the expression level of mytimacin-4 gene was significantly increased at 24 h (P < 0.05) in the mantle and increased at 48 h (P < 0.05) in the posterior adductor muscle. This finding suggested that mytimacin-4 transcripts were inducible upon pathogen infection. A minimal inhibitory concentration (MIC) assay indicated that recombinant mytimacin-4 protein had potent antimicrobial activities against gram-positive and gram-negative bacteria. Among the tested microorganisms, mytimacin-4 protein exhibited strong inhibition activities against Bacillus subtilis and Vibrio parahaemolyticus with MICs of 0.315 mu M and 0.62 mu M respectively. This study provides for the first time direct evidence of antimicrobial action of mytimacin-4 in M. galloprovincialis.
Funding OrganizationNational Natural Science Foundation of ChinaNational Natural Science Foundation of China [NSFC 41876135]
SubtypeArticle
Indexed BySCI
Language英语
WOS KeywordRICH ANTIMICROBIAL PEPTIDE ; MOLECULAR CHARACTERIZATION ; ANTIBACTERIAL PEPTIDES ; HEMOCYTES ; INVERTEBRATES ; CHALLENGE ; SEAWATER ; IMMUNITY ; BINDING ; LEECH
WOS Research AreaZoology
WOS IDWOS:000485335900010
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Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/24867
Collection中科院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室
Affiliation1.Yantai Univ, Biol Sch, Yantai 264005, Peoples R China;
2.Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res YIC, Shandong Key Lab Coastal Environm Proc,YICCAS, Yantai 264003, Shandong, Peoples R China
Recommended Citation
GB/T 7714
Lv, JS,Zhao, JM,Yang, DL,et al. Tissue distribution and functional characterization of mytimacin-4 in Mytilus galloprovincialis[J]. JOURNAL OF INVERTEBRATE PATHOLOGY,2019,166:107215.
APA Lv, JS,Zhao, JM,Yang, DL,Wu, HF,&Cong, M.(2019).Tissue distribution and functional characterization of mytimacin-4 in Mytilus galloprovincialis.JOURNAL OF INVERTEBRATE PATHOLOGY,166,107215.
MLA Lv, JS,et al."Tissue distribution and functional characterization of mytimacin-4 in Mytilus galloprovincialis".JOURNAL OF INVERTEBRATE PATHOLOGY 166(2019):107215.
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