iTRAQ-based proteomic analysis on the mitochondrial responses in gill tissues of juvenile olive flounder Paralichthys olivaceus exposed to cadmium
Lu, Z; Wang, S; Ji, CL; Li, F; Cong, M; Shan, XJ; Wu, HF
Source PublicationENVIRONMENTAL POLLUTION
ISSN0269-7491
2020-02
Volume257Pages:113591
KeywordCadmium Paralichthys olivaceus iTRAQ Proteomics Mitochondrial toxicity
MOST Discipline CatalogueEnvironmental Sciences
DOI10.1016/j.envpol.2019.113591
Contribution Rank[Lu, Zhen; Wang, Shuang; Ji, Chenglong; Li, Fei; Cong, Ming; Wu, Huifeng] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Key Lab Coastal Environm Proc,YICCAS, Yantai 264003, Peoples R China; [Ji, Chenglong; Shan, Xiujuan] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China; [Lu, Zhen; Wang, Shuang] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Department海岸带环境过程实验室
AbstractCadmium (Cd) is an important heavy metal pollutant in the Bohai Sea. Mitochondria are recognized as the key target for Cd toxicity. However, mitochondria! responses to Cd have not been fully investigated in marine fishes. In this study, the mitochondrial responses were characterized in gills of juvenile flounder Paralichthys olivaceus treated with two environmentally relevant concentrations (5 and 50 mu g/L) of Cd for 14 days by determination of mitochondrial membrane potential (MMP), observation of mitochondrial morphology and quantitative proteomic analysis. Both Cd treatments significantly decreased MMPs of mitochondria from flounder gills. Mitochondrial morphologies were altered in Cd-treated flounder samples, indicated by more and smaller mitochondria. iTRAQ-based proteomic analysis indicated that a total of 128 proteins were differentially expressed in both Cd treatments. These proteins were basically involved in various biological processes in gill mitochondria, including mitochondrial morphology and import, tricarboxylic acid (TCA) cycle, oxidative phosphorylation (OXPHOS), primary bile acid biosynthesis, stress resistance and apoptosis. These results indicated that dynamic regulations of energy homeostasis, cholesterol metabolism, stress resistance, apoptosis, and mitochondrial morphology in gill mitochondria might play significant roles in response to Cd toxicity. Overall, this study provided a global view on mitochondrial toxicity of Cd in flounder gills using iTRAQ-based proteomics. (C) 2019 Elsevier Ltd. All rights reserved.
SubtypeArticle
Indexed BySCI
Language英语
WOS KeywordVIRGINICA GMELIN BIVALVIA ; BILE-ACID SYNTHESIS ; ENERGY-METABOLISM ; ROS PRODUCTION ; COMPLEX-II ; PROTEIN ; MITOFILIN ; ACCUMULATION ; TARGET ; SAM50
WOS Research AreaEnvironmental Sciences
WOS IDWOS:000514746800091
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/24756
Collection中科院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室
Affiliation1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Key Lab Coastal Environm Proc,YICCAS, Yantai 264003, Peoples R China;
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China;
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Lu, Z,Wang, S,Ji, CL,et al. iTRAQ-based proteomic analysis on the mitochondrial responses in gill tissues of juvenile olive flounder Paralichthys olivaceus exposed to cadmium[J]. ENVIRONMENTAL POLLUTION,2020,257:113591.
APA Lu, Z.,Wang, S.,Ji, CL.,Li, F.,Cong, M.,...&Wu, HF.(2020).iTRAQ-based proteomic analysis on the mitochondrial responses in gill tissues of juvenile olive flounder Paralichthys olivaceus exposed to cadmium.ENVIRONMENTAL POLLUTION,257,113591.
MLA Lu, Z,et al."iTRAQ-based proteomic analysis on the mitochondrial responses in gill tissues of juvenile olive flounder Paralichthys olivaceus exposed to cadmium".ENVIRONMENTAL POLLUTION 257(2020):113591.
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