YIC-IR  > 中科院海岸带环境过程与生态修复重点实验室
Molecular characterization, expression and antimicrobial activities of two c-type lysozymes from manila clam Venerupis philippinarum
Yang, Dinglong1,2,3; Wang, Qing1; Cao, Ruiwen1,3; Chen, Lizhu1,3; Liu, Yongliang2; Cong, Ming1; Wu, Huifeng1; Li, Fei1; Ji, Chenglong1; Zhao, Jianmin1,2
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
2017-08-01
Volume73Pages:109-118
KeywordVenerupis Philippinarum C-type Lysozyme Antimicrobial Activity Muramidase Activity
MOST Discipline CatalogueFisheries ; Immunology ; Zoology
DOI10.1016/j.dci.2017.03.018
Contribution Rank[Yang, Dinglong; Wang, Qing; Cao, Ruiwen; Chen, Lizhu; Cong, Ming; Wu, Huifeng; Li, Fei; Ji, Chenglong; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc, Yantai 264003, Shandong, Peoples R China; [Yang, Dinglong; Liu, Yongliang; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China; [Yang, Dinglong; Cao, Ruiwen; Chen, Lizhu] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Department海岸带生物资源高效利用研究与发展中心
AbstractLysozymes play an important role in the innate immune responses with which mollusks respond to bacterial invasion through its lytic activity. In the present study, two c-type lysozymes (designed as VpCLYZ-1 and VpCLYZ-2, respectively) were identified and characterized from the manila clam Venerupis philippinarum. The full-length cDNA of VpCLYZ-1 and VpCLYZ-2 was of 629 and 736 bp, encoding a polypeptide of 156 and153 amino acid residues, respectively. The deduced amino acid sequences of VpCLYZs showed high similarity to other known invertebrate c-type lysozymes. Multiple alignments and phylogenetic relationship strongly suggested that VpCLYZ-1 and VpCLYZ-2 belonged to the c-type lysozyme family. Both VpCLYZ-1 and VpCLYZ-2 transcripts were constitutively expressed in a wide range of tissues with different levels. The VpCLYZ-1 transcript was dominantly expressed in hepatopancreas and hemocytes, while VpCLYZ-2 transcript was mainly expressed in the tissues of hepatopancreas and gills. Both the mRNA expression of VpCLYZ-1 and VpCLYZ-2 was significantly up-regulated at 12 h post Vibrio anguillarum challenge. The recombinant VpCLYZ-1 and VpCLYZ-2 (designed as rVpCLYZ-1 and rVpCLYZ-2) exhibited lytic activity against all tested bacteria, and rVpCLYZ-1 showed higher activities than rVpCLYZ-2 in killing Micrococcus luteus and V anguillarum. Overall, our results suggested that VpCLYZ-1 and VpCLYZ-2 belonged to the c-type lysozyme family, and played important roles in the immune responses of manila clam, especially in the elimination of pathogens. (C) 2017 Elsevier Ltd. All rights reserved.
Funding OrganizationNational Natural Science Foundation of China(41476126) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA11020702 ; Key Research Program of the Chinese Academy of Sciences(KZZD-EW-14) ; Youth Innovation Promotion Association, CAS(2016196) ; XDA11020305)
SubtypeArticle
Indexed BySCI
Language英语
WOS KeywordBROWN RING DISEASE ; RUDITAPES-PHILIPPINARUM ; ANTIBACTERIAL ACTIVITY ; EASTERN OYSTER ; CLONING ; IDENTIFICATION ; PURIFICATION ; HEMOCYTES ; SYSTEM ; ANDREI
WOS Research AreaImmunology ; Zoology
WOS IDWOS:000401380400013
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/22512
Collection中科院海岸带环境过程与生态修复重点实验室
Affiliation1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc, Yantai 264003, Shandong, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Yang, Dinglong,Wang, Qing,Cao, Ruiwen,et al. Molecular characterization, expression and antimicrobial activities of two c-type lysozymes from manila clam Venerupis philippinarum[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2017,73:109-118.
APA Yang, Dinglong.,Wang, Qing.,Cao, Ruiwen.,Chen, Lizhu.,Liu, Yongliang.,...&Zhao, Jianmin.(2017).Molecular characterization, expression and antimicrobial activities of two c-type lysozymes from manila clam Venerupis philippinarum.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,73,109-118.
MLA Yang, Dinglong,et al."Molecular characterization, expression and antimicrobial activities of two c-type lysozymes from manila clam Venerupis philippinarum".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 73(2017):109-118.
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