Molecular characterization, expression and antimicrobial activities of two c-type lysozymes from manila clam Venerupis philippinarum
Yang, Dinglong1,2,3; Wang, Qing1; Cao, Ruiwen1,3; Chen, Lizhu1,3; Liu, Yongliang2; Cong, Ming1; Wu, Huifeng1; Li, Fei1; Ji, Chenglong1; Zhao, Jianmin1,2
2017-08-01
发表期刊DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
卷号73页码:109-118
摘要Lysozymes play an important role in the innate immune responses with which mollusks respond to bacterial invasion through its lytic activity. In the present study, two c-type lysozymes (designed as VpCLYZ-1 and VpCLYZ-2, respectively) were identified and characterized from the manila clam Venerupis philippinarum. The full-length cDNA of VpCLYZ-1 and VpCLYZ-2 was of 629 and 736 bp, encoding a polypeptide of 156 and153 amino acid residues, respectively. The deduced amino acid sequences of VpCLYZs showed high similarity to other known invertebrate c-type lysozymes. Multiple alignments and phylogenetic relationship strongly suggested that VpCLYZ-1 and VpCLYZ-2 belonged to the c-type lysozyme family. Both VpCLYZ-1 and VpCLYZ-2 transcripts were constitutively expressed in a wide range of tissues with different levels. The VpCLYZ-1 transcript was dominantly expressed in hepatopancreas and hemocytes, while VpCLYZ-2 transcript was mainly expressed in the tissues of hepatopancreas and gills. Both the mRNA expression of VpCLYZ-1 and VpCLYZ-2 was significantly up-regulated at 12 h post Vibrio anguillarum challenge. The recombinant VpCLYZ-1 and VpCLYZ-2 (designed as rVpCLYZ-1 and rVpCLYZ-2) exhibited lytic activity against all tested bacteria, and rVpCLYZ-1 showed higher activities than rVpCLYZ-2 in killing Micrococcus luteus and V anguillarum. Overall, our results suggested that VpCLYZ-1 and VpCLYZ-2 belonged to the c-type lysozyme family, and played important roles in the immune responses of manila clam, especially in the elimination of pathogens. (C) 2017 Elsevier Ltd. All rights reserved.
关键词Venerupis Philippinarum C-type Lysozyme Antimicrobial Activity Muramidase Activity
DOI10.1016/j.dci.2017.03.018
项目资助者National Natural Science Foundation of China(41476126) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA11020702 ; Key Research Program of the Chinese Academy of Sciences(KZZD-EW-14) ; Youth Innovation Promotion Association, CAS(2016196) ; XDA11020305)
收录类别SCI
关键词[WOS]BROWN RING DISEASE ; RUDITAPES-PHILIPPINARUM ; ANTIBACTERIAL ACTIVITY ; EASTERN OYSTER ; CLONING ; IDENTIFICATION ; PURIFICATION ; HEMOCYTES ; SYSTEM ; ANDREI
文章类型Article
语种英语
WOS研究方向Immunology ; Zoology
WOS记录号WOS:000401380400013
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/22512
专题中科院海岸带环境过程与生态修复重点实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc, Yantai 264003, Shandong, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位中国科学院烟台海岸带研究所
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Yang, Dinglong,Wang, Qing,Cao, Ruiwen,et al. Molecular characterization, expression and antimicrobial activities of two c-type lysozymes from manila clam Venerupis philippinarum[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2017,73:109-118.
APA Yang, Dinglong.,Wang, Qing.,Cao, Ruiwen.,Chen, Lizhu.,Liu, Yongliang.,...&Zhao, Jianmin.(2017).Molecular characterization, expression and antimicrobial activities of two c-type lysozymes from manila clam Venerupis philippinarum.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,73,109-118.
MLA Yang, Dinglong,et al."Molecular characterization, expression and antimicrobial activities of two c-type lysozymes from manila clam Venerupis philippinarum".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 73(2017):109-118.
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