Molecular characterization, expression and antimicrobial activities of two c-type lysozymes from manila clam Venerupis philippinarum

doi:10.1016/j.dci.2017.03.018

	Molecular characterization, expression and antimicrobial activities of two c-type lysozymes from manila clam Venerupis philippinarum
	Yang, Dinglong1,2,3 ; Wang, Qing1 ; Cao, Ruiwen 1,3; Chen, Lizhu 1,3; Liu, Yongliang 2; Cong, Ming1 ; Wu, Huifeng1 ; Li, Fei1 ; Ji, Chenglong1 ; Zhao, Jianmin1,2
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN	0145-305X
	2017-08-01
卷号	73 页码:109-118
关键词	Venerupis Philippinarum C-type Lysozyme Antimicrobial Activity Muramidase Activity
研究领域	Fisheries ; Immunology ; Zoology
DOI	10.1016/j.dci.2017.03.018
产权排序	[Yang, Dinglong; Wang, Qing; Cao, Ruiwen; Chen, Lizhu; Cong, Ming; Wu, Huifeng; Li, Fei; Ji, Chenglong; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc, Yantai 264003, Shandong, Peoples R China; [Yang, Dinglong; Liu, Yongliang; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China; [Yang, Dinglong; Cao, Ruiwen; Chen, Lizhu] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
作者部门	海岸带生物资源高效利用研究与发展中心
英文摘要	Lysozymes play an important role in the innate immune responses with which mollusks respond to bacterial invasion through its lytic activity. In the present study, two c-type lysozymes (designed as VpCLYZ-1 and VpCLYZ-2, respectively) were identified and characterized from the manila clam Venerupis philippinarum. The full-length cDNA of VpCLYZ-1 and VpCLYZ-2 was of 629 and 736 bp, encoding a polypeptide of 156 and153 amino acid residues, respectively. The deduced amino acid sequences of VpCLYZs showed high similarity to other known invertebrate c-type lysozymes. Multiple alignments and phylogenetic relationship strongly suggested that VpCLYZ-1 and VpCLYZ-2 belonged to the c-type lysozyme family. Both VpCLYZ-1 and VpCLYZ-2 transcripts were constitutively expressed in a wide range of tissues with different levels. The VpCLYZ-1 transcript was dominantly expressed in hepatopancreas and hemocytes, while VpCLYZ-2 transcript was mainly expressed in the tissues of hepatopancreas and gills. Both the mRNA expression of VpCLYZ-1 and VpCLYZ-2 was significantly up-regulated at 12 h post Vibrio anguillarum challenge. The recombinant VpCLYZ-1 and VpCLYZ-2 (designed as rVpCLYZ-1 and rVpCLYZ-2) exhibited lytic activity against all tested bacteria, and rVpCLYZ-1 showed higher activities than rVpCLYZ-2 in killing Micrococcus luteus and V anguillarum. Overall, our results suggested that VpCLYZ-1 and VpCLYZ-2 belonged to the c-type lysozyme family, and played important roles in the immune responses of manila clam, especially in the elimination of pathogens. (C) 2017 Elsevier Ltd. All rights reserved.
文章类型	Article
资助机构	National Natural Science Foundation of China(41476126) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA11020702 ; Key Research Program of the Chinese Academy of Sciences(KZZD-EW-14) ; Youth Innovation Promotion Association, CAS(2016196) ; XDA11020305)
收录类别	SCI
语种	英语
关键词[WOS]	BROWN RING DISEASE ; RUDITAPES-PHILIPPINARUM ; ANTIBACTERIAL ACTIVITY ; EASTERN OYSTER ; CLONING ; IDENTIFICATION ; PURIFICATION ; HEMOCYTES ; SYSTEM ; ANDREI
研究领域[WOS]	Immunology ; Zoology
WOS记录号	WOS:000401380400013
引用统计	被引频次：23[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/22512
专题	中国科学院海岸带环境过程与生态修复重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc, Yantai 264003, Shandong, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Yang, Dinglong,Wang, Qing,Cao, Ruiwen,et al. Molecular characterization, expression and antimicrobial activities of two c-type lysozymes from manila clam Venerupis philippinarum[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2017,73:109-118.
APA	Yang, Dinglong.,Wang, Qing.,Cao, Ruiwen.,Chen, Lizhu.,Liu, Yongliang.,...&Zhao, Jianmin.(2017).Molecular characterization, expression and antimicrobial activities of two c-type lysozymes from manila clam Venerupis philippinarum.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,73,109-118.
MLA	Yang, Dinglong,et al."Molecular characterization, expression and antimicrobial activities of two c-type lysozymes from manila clam Venerupis philippinarum".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 73(2017):109-118.