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重组别藻蓝蛋白的超快能量传递过程 期刊论文
海洋学报, 2021, 卷号: 43, 期号: 9, 页码: 115-125
作者:  甄张赫;  朱锐丹;  秦松;  陈海龙;  蒲洋;  翁羽翔;  李文军
Adobe PDF(2054Kb)  |  收藏  |  浏览/下载：298/55  |  提交时间：2021/12/01
别藻蓝蛋白  重组异源表达  能量传递  稳态光谱  瞬态光谱  
基于分子印迹的荧光传感技术及其应用研究 期刊论文
中国科学:化学, 2020, 卷号: 50, 期号: 04, 页码: 463-475
作者:  韩笑笑;  齐骥;  宋志花;  石雅君;  刘丰;  张昱;  韩京龙;  徐惠忠;  李博伟
浏览  |  Adobe PDF(5473Kb)  |  收藏  |  浏览/下载：806/392  |  提交时间：2020/07/08
分子印迹荧光传感器  单发射型  比率型  农药残留  雌激素  抗生素  
藻类特有的捕光色素蛋白——藻红蛋白的结构、功能及应用 期刊论文
科学通报, 2020, 卷号: 65, 期号: 07, 页码: 565-576
作者:  臧帆;  秦松;  马丞博;  李文军;  林剑
Adobe PDF(1397Kb)  |  收藏  |  浏览/下载：727/241  |  提交时间：2020/07/08
藻红蛋白  结构功能  制备工艺  光学活性  生物活性  
环境友好型比率荧光微流控纸芯片快速检测苯醚甲环唑 期刊论文
中国科学:化学, 2020, 卷号: 50, 期号: 03, 页码: 393-405
作者:  马昕;  郝帼英;  张忠;  李金花;  杨兴斌;  陈令新
浏览  |  Adobe PDF(13626Kb)  |  收藏  |  浏览/下载：454/188  |  提交时间：2020/07/08
分子印迹  碳量子点  比率荧光  苯醚甲环唑  微流控纸芯片  
藻类特有的捕光色素蛋白藻红蛋白的结构、功能及应用 期刊论文
科学通报, 2020, 卷号: 65, 期号: 7, 页码: 565-576
作者:  臧帆;  秦松;  马丞博;  李文军;  林剑
Adobe PDF(1396Kb)  |  收藏  |  浏览/下载：191/51  |  提交时间：2021/12/01
藻红蛋白  结构功能  制备工艺  光学活性  生物活性  
基于分子印迹的荧光传感技术及其应用研究 期刊论文
中国科学. 化学, 2020, 卷号: 50, 期号: 4, 页码: 463-475
作者:  韩笑笑;  齐骥;  宋志花;  石雅君;  刘丰;  张昱;  韩京龙;  徐惠忠;  李博伟
浏览  |  Adobe PDF(5473Kb)  |  收藏  |  浏览/下载：432/235  |  提交时间：2021/12/01
分子印迹荧光传感器  单发射型  比率型  农药残留  雌激素  抗生素  
环境友好型比率荧光微流控纸芯片快速检测苯醚甲环唑 期刊论文
中国科学. 化学, 2020, 卷号: 50, 期号: 3, 页码: 393-405
作者:  马昕;  郝帼英;  张忠;  李金花;  杨兴斌;  陈令新
浏览  |  Adobe PDF(13626Kb)  |  收藏  |  浏览/下载：324/117  |  提交时间：2021/12/01
分子印迹  碳量子点  比率荧光  苯醚甲环唑  微流控纸芯片  
基于印迹聚合物的微流控量子点纸基芯片检测环境中的镉、铅离子 期刊论文
分析试验室, 2019, 卷号: 38, 期号: 01, 页码: 7-12
作者:  王冠;  齐骥;  戚安金;  周俊瑞;  李博伟;  陈令新
浏览  |  Adobe PDF(417Kb)  |  收藏  |  浏览/下载：518/224  |  提交时间：2020/07/08
碲化镉量子点  纸芯片  离子印迹聚合物  镉和铅离子  
藻胆蛋白生物合成研究进展 期刊论文
科学通报, 2019, 卷号: 64, 期号: 01, 页码: 49-59
作者:  马丞博;  秦松;  李文军;  葛保胜
Adobe PDF(12113Kb)  |  收藏  |  浏览/下载：446/76  |  提交时间：2020/07/08
藻胆蛋白  生物合成  体外重组  抗氧化  荧光  
食用蔬菜能吸收和积累微塑料 期刊论文
科学通报, 2019, 卷号: 64, 期号: 9, 页码: 928-934
作者:  李连祯;  周倩;  尹娜;  涂晨;  骆永明
收藏  |  浏览/下载：493/0  |  提交时间：2020/06/17
微塑料  生菜  聚苯乙烯微球  吸收  积累  健康风险  microplastics  lettuce  polystyrene microbeads  uptake  accumulation  human health risk  Microplastic (MP, 100 nm-5 mm) may present an attributable risk to ecosystem and human health, and its pollution has become a global environmental concern. Despite a wealth of information on the accumulation of MPs in aquatic species, there is no information on the uptake and accumulation of MPs by higher plants. Terrestrial edible plants are directly exposed to MPs when agricultural soil was applied with organic manure, sewage sludge as fertilizer or plastic mulching. In this paper, the uptake of two sizes of polystyrene (PS) microbeads (0.2 and 1.0 mum) and then their distribution and migration in an edible plant lettuce were firstly investigated based on laboratory experiments. We used fluorescent markers to track PS microbeads in plant tissues and found fluorescence to be a sensitive and reliable detection method. Sections from untreated control lettuce showed no autofluorescence. When roots were treated with fluorescently labeled PS microbeads, the microbeads could be identified by its fluorescence. Our main study investigated the uptake of 0.2 mum beads, as few luminescence signals were observed in lettuce roots for 1.0 mum beads in our experiment. We observed that 0.2 mum fluorescent microbeads were extracellularly trapped in the root cap mucilage (which is a highly hydrated polysaccharide) and a dark green tip (which was typical of lettuce roots exposed to label PS beads) was usually visible to the naked eye. Confocal images revealed that the PS luminescence signals were mainly located in the vascular system and on the cell walls of the cortex tissue of the roots, indicated that the beads passed through the intercellular space via the apoplastic transport system. Once inside the central cylinder, the 0.2 mum PS beads were transferred from the roots to the stems and leaves via the vascular system following the transpiration stream. We also observed that the PS beads adhered to one another and self-assembled systematically into grape-like and (chain) string-like clusters in the intercellular space of the root and stem vascular tissue of lettuce plant. In contrast to the root and stem, PS beads were dispersed in the leaf tissue. Here, for the first time we provide evidence of the adherence, uptake, accumulation, and translocation of submicrometer MPs within an edible plant. Our findings highlight the previously underappreciated human exposure pathway to MPs through the consumption of contaminated crops and emphasize the need for new management strategies to control the release of MPs waste products into the terrestrial environment. Ultimately, the potential impacts of low range sized MPs on food safety of crop plants and human health need to be urgently considered.