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Facile fluorescence detection of malachite green in fish using molecularly imprinted polymers doped CdTe quantum dots based system 期刊论文
FOOD CHEMISTRY, 2024, 卷号: 442, 页码: 10
作者:  Zhang, Yimeng;  Wang, Chuanliang;  Wei, Guo;  Wang, Xuesong;  Liu, Wanhui;  Yang, Gangqiang;  Zhang, Peng;  Li, Qinglian;  Geng, Xuhui;  Chen, Lingxin;  Song, Zhihua
收藏  |  浏览/下载：97/0  |  提交时间：2024/05/07
Fluorescence detection  CdTe quantum dots  Molecularly imprinted polymers  Malachite green  Fish tissues  
碳氮稳定同位素比值在潮间带大型底栖动物组织间差异性研究 期刊论文
海洋学报, 2019, 卷号: 41, 期号: 4, 页码: 74-82
作者:  李宝泉
Adobe PDF(970Kb)  |  收藏  |  浏览/下载：373/86  |  提交时间：2020/06/17
潮间带  大型底栖动物  碳氮稳定同位素  食源和营养级分析  采样部位选取  intertidal zone  macrobenthos  carbon and nitrogen stable isotopes  food sources and trophic levels study,sampling parts  The technique of stable isotope analysis has been widely applied for the field of food web studies in recent years.However,few researches concerned about the impacts of different tissues used for analysis on the food web building.With the purpose of accuracy and comparability in the food web studies,the sampling tissues should be standardized.In this study,10macrobenthic species from the intertidal zone of the Yellow River Delta and Yantai were chosen as the target object to test the isotopic characteristic variations ofdelta~(13)C anddelta15 N at different body parts (whole bodies,muscles and gills).Our results show thatdelta~(13)C anddelta15 N values vary in different sampling tissues for most species,which indicates the different tissues used for analysis impact the food web building.To achieve a more accuracy and comparability result,suitable tissue should be primarily chosen for their food sources and trophic levels analysis.Namely,when the organism as predator,their muscles should first be chosen,for examples,adductor and foot muscles of mollusks,leg muscles of crabs and back muscles of fishes  when the organism as prey, most of their tissues,except for indigestible part(for polychaetes,the digestive tract contents should be removed) should be chosen for analysis on food sources and trophic levels of the secondary consumer,for example,whole soft parts of mollusks,the mixture of muscles and gills of crabs.  
食用蔬菜能吸收和积累微塑料 期刊论文
科学通报, 2019, 卷号: 64, 期号: 9, 页码: 928-934
作者:  李连祯;  周倩;  尹娜;  涂晨;  骆永明
收藏  |  浏览/下载：536/0  |  提交时间：2020/06/17
微塑料  生菜  聚苯乙烯微球  吸收  积累  健康风险  microplastics  lettuce  polystyrene microbeads  uptake  accumulation  human health risk  Microplastic (MP, 100 nm-5 mm) may present an attributable risk to ecosystem and human health, and its pollution has become a global environmental concern. Despite a wealth of information on the accumulation of MPs in aquatic species, there is no information on the uptake and accumulation of MPs by higher plants. Terrestrial edible plants are directly exposed to MPs when agricultural soil was applied with organic manure, sewage sludge as fertilizer or plastic mulching. In this paper, the uptake of two sizes of polystyrene (PS) microbeads (0.2 and 1.0 mum) and then their distribution and migration in an edible plant lettuce were firstly investigated based on laboratory experiments. We used fluorescent markers to track PS microbeads in plant tissues and found fluorescence to be a sensitive and reliable detection method. Sections from untreated control lettuce showed no autofluorescence. When roots were treated with fluorescently labeled PS microbeads, the microbeads could be identified by its fluorescence. Our main study investigated the uptake of 0.2 mum beads, as few luminescence signals were observed in lettuce roots for 1.0 mum beads in our experiment. We observed that 0.2 mum fluorescent microbeads were extracellularly trapped in the root cap mucilage (which is a highly hydrated polysaccharide) and a dark green tip (which was typical of lettuce roots exposed to label PS beads) was usually visible to the naked eye. Confocal images revealed that the PS luminescence signals were mainly located in the vascular system and on the cell walls of the cortex tissue of the roots, indicated that the beads passed through the intercellular space via the apoplastic transport system. Once inside the central cylinder, the 0.2 mum PS beads were transferred from the roots to the stems and leaves via the vascular system following the transpiration stream. We also observed that the PS beads adhered to one another and self-assembled systematically into grape-like and (chain) string-like clusters in the intercellular space of the root and stem vascular tissue of lettuce plant. In contrast to the root and stem, PS beads were dispersed in the leaf tissue. Here, for the first time we provide evidence of the adherence, uptake, accumulation, and translocation of submicrometer MPs within an edible plant. Our findings highlight the previously underappreciated human exposure pathway to MPs through the consumption of contaminated crops and emphasize the need for new management strategies to control the release of MPs waste products into the terrestrial environment. Ultimately, the potential impacts of low range sized MPs on food safety of crop plants and human health need to be urgently considered.  
Evaluation Selenocysteine Protective Effect in Carbon Disulfide Induced Hepatitis with a Mitochondrial Targeting Ratiometric Near-Infrared Fluorescent Probe 期刊论文
ANALYTICAL CHEMISTRY, 2018, 卷号: 90, 期号: 13, 页码: 8108-8115
作者:  Han, Xiaoyue;  Wang, Rui;  Song, Xinyu;  Yu, Fabiao;  Chen, Lingxin
浏览  |  Adobe PDF(6933Kb)  |  收藏  |  浏览/下载：485/282  |  提交时间：2020/07/08
PROTEIN-KINASE PATHWAYS  HUMAN HEALTH  SELENIUM  SELENOLS  CELLS  SELENOPROTEINS  SPECTROMETRY  DYSFUNCTION  TISSUES  PLASMA  
A reversible fluorescent probe based on C=N isomerization for the selective detection of formaldehyde in living cells and in vivo 期刊论文
ANALYST, 2018, 卷号: 143, 期号: 2, 页码: 429-439
作者:  Song, Xinyu;  Han, Xiaoyue;  Yu, Fabiao;  Zhang, Jinjin;  Chen, Lingxin;  Lv, Changjun
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AZA-COPE REACTION  ENDOGENOUS FORMALDEHYDE  HYDROGEN POLYSULFIDES  IMAGING FORMALDEHYDE  SILVER NANOCLUSTERS  DESIGN STRATEGIES  FLUOROGENIC PROBE  TISSUES  MECHANISM  LEVEL  
A two-photon ratiometric fluorescent probe for the synergistic detection of the mitochondrial SO2/HClO crosstalk in cells and in vivo 期刊论文
JOURNAL OF MATERIALS CHEMISTRY B, 2017, 卷号: 5, 期号: 42, 页码: 8389-8398
作者:  Dou, K;  Chen, G;  Yu, FB;  Sun, ZW;  Li, GL;  Zhao, XE;  Chen, LX;  You, JM
浏览  |  Adobe PDF(2706Kb)  |  收藏  |  浏览/下载：533/279  |  提交时间：2020/07/08
SULFUR-DIOXIDE DERIVATIVES  LIVING CELLS  HYPOCHLOROUS ACID  OXIDATIVE STRESS  HYDROGEN-SULFIDE  BIOIMAGING APPLICATIONS  PARKINSONS-DISEASE  REACTIVE OXYGEN  LIVE-CELL  TISSUES  
The first molluscan TCTP in Venerupis philippinarum: Molecular cloning and expression analysis 期刊论文
FISH & SHELLFISH IMMUNOLOGY, 2010, 卷号: 29, 期号: 3, 页码: 530-533
作者:  Li, Chenghua;  Qiu, Limei;  Ning, Xuanxuan;  Chen, Aiqin;  Qin, Song;  Wu, Huifeng;  Zhao, Jianmin
Adobe PDF(451Kb)  |  收藏  |  浏览/下载：1278/360  |  提交时间：2011/07/14
Venerupis Philippinarum  Tctp  Tissues Expression  Time-course Expression