| Evaluation Selenocysteine Protective Effect in Carbon Disulfide Induced Hepatitis with a Mitochondrial Targeting Ratiometric Near-Infrared Fluorescent Probe | |
Han, Xiaoyue; Wang, Rui; Song, Xinyu; Yu, Fabiao; Chen, Lingxin
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| 发表期刊 | ANALYTICAL CHEMISTRY
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| ISSN | 0003-2700 |
| 2018-07-03 | |
| 卷号 | 90期号:13页码:8108-8115 |
| 关键词 | PROTEIN-KINASE PATHWAYS HUMAN HEALTH SELENIUM SELENOLS CELLS SELENOPROTEINS SPECTROMETRY DYSFUNCTION TISSUES PLASMA |
| 研究领域 | Chemistry |
| DOI | 10.1021/acs.analchem.8b01306 |
| 产权排序 | [Han, Xiaoyue; Wang, Rui; Song, Xinyu; Yu, Fabiao; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Han, Xiaoyue] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Chen, Lingxin] Yantai Univ, Coll Chem & Chem Engn, Yantai 264005, Peoples R China |
| 作者部门 | 海岸带环境工程技术研究与发展中心 |
| 英文摘要 | As important active sites of oxidoreductase in mitochondria, selenocysteine (Sec) takes the responsibility for cytoprotective effect and intracellular redox homeostasis. Carbon disulfide (CS2) is a common solvent in industry, which can inhibit the activities of oxidoreductase and induce oxidative stress. It is necessary to investigate the cytoprotective effect of Sec against CS2 exposure. After integrated, the response moiety 2,4-dinitrobenzenesulfonamide and mitochondrial targeting moiety into the near-infrared heptamethine cyanine fluorophore, we develop a mitochondrial targeting near-infrared ratiometric fluorescent probe Mito-diNO(2) for the selective and sensitive analysis of Sec concentration fluctuations in living cells and in mice models under the stimulation of CS2. The probe can effectively accumulate in mitochondria and selectively detect the endogenous Sec concentrations in BRL 3A, RH-35, HL-7702, HepG2, and SMMC-7721 cell lines. The results indicate that CS2 exposure can lead to a decrease of Sec level and result in mitochondrial related acute inflammation. The exogenous supplement of Sec can protect cells from oxidative damage and reduce the symptoms of inflammation. We also establish CS2 induced acute and chronic hepatitis mice models to examine the tissue toxicity of CS2 and cytoprotection of Sec in liver. The organism can increase the concentration of Sec to deal with the damage caused by CS2 in acute hepatitis mice model. Also the exogenous supplement of Sec for the two mice models can effectively defend the CS2 induced liver damage. The real-time imaging of Sec concentrations in liver can be used to assess the degrees of liver injury during CS2 poisoning. The above applications make our probe a potential candidate for the clinical accurate diagnosis and treatment of CS2 poisoning. |
| 文章类型 | Article |
| 资助机构 | National Nature Science Foundation of China [21775162, 21575159, 41776110, 21405172] ; program of Youth Innovation Promotion Association, CAS [2015170] ; State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, CAS [KF2016-22] ; Instrument Developing Project of the Chinese Academy of Sciences [YZ201662] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | PROTEIN-KINASE PATHWAYS ; HUMAN HEALTH ; SELENIUM ; SELENOLS ; CELLS ; SELENOPROTEINS ; SPECTROMETRY ; DYSFUNCTION ; TISSUES ; PLASMA |
| 研究领域[WOS] | Chemistry, Analytical |
| WOS记录号 | WOS:000438008600045 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/24439 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心 中国科学院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 3.Yantai Univ, Coll Chem & Chem Engn, Yantai 264005, Peoples R China |
| 推荐引用方式 GB/T 7714 | Han, Xiaoyue,Wang, Rui,Song, Xinyu,et al. Evaluation Selenocysteine Protective Effect in Carbon Disulfide Induced Hepatitis with a Mitochondrial Targeting Ratiometric Near-Infrared Fluorescent Probe[J]. ANALYTICAL CHEMISTRY,2018,90(13):8108-8115. |
| APA | Han, Xiaoyue,Wang, Rui,Song, Xinyu,Yu, Fabiao,&Chen, Lingxin.(2018).Evaluation Selenocysteine Protective Effect in Carbon Disulfide Induced Hepatitis with a Mitochondrial Targeting Ratiometric Near-Infrared Fluorescent Probe.ANALYTICAL CHEMISTRY,90(13),8108-8115. |
| MLA | Han, Xiaoyue,et al."Evaluation Selenocysteine Protective Effect in Carbon Disulfide Induced Hepatitis with a Mitochondrial Targeting Ratiometric Near-Infrared Fluorescent Probe".ANALYTICAL CHEMISTRY 90.13(2018):8108-8115. |
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