Both released silver ions and particulate Ag contribute to the toxicity of AgNPs to earthworm Eisenia fetida

doi:10.3109/17435390.2014.976851

	Both released silver ions and particulate Ag contribute to the toxicity of AgNPs to earthworm Eisenia fetida
	Li, LZ; Wu, HF; Peijnenburg, WJGM; van Gestel, CAM; Wu, HF (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai 264003, Shandong, Peoples R China. hfwu@yic.ac.cn
发表期刊	NANOTOXICOLOGY
ISSN	1743-5390
	2015
卷号	9 期号:6 页码:792-801
关键词	Eisenia Fetida Metabolomics Nanoparticles Nanotoxicology
DOI	10.3109/17435390.2014.976851
产权排序	[Li, Lianzhen; Wu, Huifeng] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai 264003, Shandong, Peoples R China; [Li, Lianzhen; Wu, Huifeng] YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai, Shandong, Peoples R China; [Peijnenburg, Willie J. G. M.] Natl Inst Publ Hlth & Environm, Ctr Safety Subst & Prod, NL-3720 BA Bilthoven, Netherlands; [Peijnenburg, Willie J. G. M.] Leiden Univ, Inst Environm Sci CML, Leiden, Netherlands; [van Gestel, Cornelis A. M.] Vrije Univ Amsterdam, Fac Earth & Life Sci, Dept Ecol Sci, Amsterdam, Netherlands
作者部门	中科院海岸带环境过程与生态修复重点实验室
英文摘要	To disentangle the contribution of ionic and nanoparticulate Ag to the overall toxicity to the earthworm Eisenia fetida, a semi-permeable membrane strategy was used to separate Ag+ released from silver nanoparticles (AgNPs) in an aqueous exposure. Internal Ag fractionation, activities of antioxidant enzymes and metabolites in E. fetida were determined after 96 h of exposure to two sizes of polyvinylpyrrolidone-coated AgNPs. The response of the antioxidant system combined with the content of malondialdehyde indicated that the Ag+ released from AgNPs induced significant oxidative stress to the earthworms. Ag accumulated from AgNPs was predominantly associated with the granules and cell membrane compartments, whereas dissolved Ag was localized in the cytosol-containing fraction. In both Ag+ exposures, two intermediates in the Krebs cycle, succinate and fumarate, were significantly elevated and depleted, respectively. A similar alteration pattern was seen in groups exposed to both smaller AgNPs (S AgNP, 10 nm) and larger AgNP (L AgNP, 40 nm), indicating that these effects in E. fetida were induced by exposure to released Ag+. In addition, unique metabolic responses including decreased malate and glucose levels in S AgNP-exposed earthworms could be associated with exposure to nanoparticulate silver. Increased leucine and arginine and decreased ATP and inosine levels were observed in L AgNP exposures only, which clearly demonstrated a size-specific effect of AgNPs. Collectively, this study provided strong evidence that nanosilver acts by a different mechanism than ionic silver to cause acute toxicity to E. fetida, but further verification under different environmental conditions is needed.
文章类型	Article
收录类别	SCI
语种	英语
关键词[WOS]	HUMAN HEPATOMA-CELLS ; DAPHNIA-MAGNA ; NANOPARTICLES ; EXPOSURE ; METABOLOMICS ; INTERNALIZATION ; PHENANTHRENE ; DISSOLUTION ; MECHANISMS ; RESPONSES
研究领域[WOS]	Science & Technology - Other Topics ; Toxicology
WOS记录号	WOS:000361330200012
引用统计	被引频次：38[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/9993
专题	中国科学院海岸带环境过程与生态修复重点实验室
通讯作者	Wu, HF (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai 264003, Shandong, Peoples R China. hfwu@yic.ac.cn
推荐引用方式 GB/T 7714	Li, LZ,Wu, HF,Peijnenburg, WJGM,et al. Both released silver ions and particulate Ag contribute to the toxicity of AgNPs to earthworm Eisenia fetida[J]. NANOTOXICOLOGY,2015,9(6):792-801.
APA	Li, LZ,Wu, HF,Peijnenburg, WJGM,van Gestel, CAM,&Wu, HF .(2015).Both released silver ions and particulate Ag contribute to the toxicity of AgNPs to earthworm Eisenia fetida.NANOTOXICOLOGY,9(6),792-801.
MLA	Li, LZ,et al."Both released silver ions and particulate Ag contribute to the toxicity of AgNPs to earthworm Eisenia fetida".NANOTOXICOLOGY 9.6(2015):792-801.

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