YIC-IR  > 中科院海岸带环境过程与生态修复重点实验室
iTRAQ-based quantitative proteomic analyses on the gender-specific responses in mussel Mytilus galloprovincialis to tetrabromobisphenol A
Ji, Chenglong1; Wu, Huifeng1; Wei, Lei1,2; Zhao, Jianmin1; Wu, HF (reprint author), Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China. hfwu@yic.ac.cn
2014-12-01
Source PublicationAQUATIC TOXICOLOGY
ISSN0166-445X
Volume157Pages:30-40
Contribution Rank[Ji, Chenglong; Wu, Huifeng; Wei, Lei; Zhao, Jianmin] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Wei, Lei] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
AbstractTetrabromobisphenol A (TBBPA) accounts for the largest production of brominated flame-retardants (BFRs) along the Laizhou Bay in China and is the most widely used BFR in industrial products. It can induce diverse toxicities including hepatotoxicity, nephrotoxicity, neurotoxicity and endocrine disrupting effects in mammalian and fish models. In this work, we applied iTRAQ-based proteomics to investigate the gender-specific responses in mussel Mytilus galloprovincialis to TBBPA. Thirty-one proteins were differentially expressed in hepatopancreas between male and female mussels, which clearly indicated the biological differences between male and female mussels at the protein level. After exposure of TBBPA (18.4 nmol/L) for one month, a total of 60 proteins were differentially expressed in response to the TBBPA treatment in mussel hepatopancreas, among which 33 and 29 proteins were significantly altered in TBBPA-treated male and female mussel samples, respectively. Only two of the 60 proteins were commonly altered in both male and female mussel samples exposed to TBBPA. Based on KEGG analysis, these differentially expressed proteins of TBBPA-induced effects were assigned to several groups, including cytoskeleton, reproduction and development, metabolism, signal transduction, gene expression, stress response and apoptosis. Overall, results indicated that TBBPA exposure could induce apoptosis, oxidative and immune stresses and disruption in energy, protein and lipid metabolisms in both male and female mussels with different mechanisms. This work suggested that the gender differences should be considered in ecotoxicoproteomics. (C) 2014 Elsevier B.V. All rights reserved.; Tetrabromobisphenol A (TBBPA) accounts for the largest production of brominated flame-retardants (BFRs) along the Laizhou Bay in China and is the most widely used BFR in industrial products. It can induce diverse toxicities including hepatotoxicity, nephrotoxicity, neurotoxicity and endocrine disrupting effects in mammalian and fish models. In this work, we applied iTRAQ-based proteomics to investigate the gender-specific responses in mussel Mytilus galloprovincialis to TBBPA. Thirty-one proteins were differentially expressed in hepatopancreas between male and female mussels, which clearly indicated the biological differences between male and female mussels at the protein level. After exposure of TBBPA (18.4 nmol/L) for one month, a total of 60 proteins were differentially expressed in response to the TBBPA treatment in mussel hepatopancreas, among which 33 and 29 proteins were significantly altered in TBBPA-treated male and female mussel samples, respectively. Only two of the 60 proteins were commonly altered in both male and female mussel samples exposed to TBBPA. Based on KEGG analysis, these differentially expressed proteins of TBBPA-induced effects were assigned to several groups, including cytoskeleton, reproduction and development, metabolism, signal transduction, gene expression, stress response and apoptosis. Overall, results indicated that TBBPA exposure could induce apoptosis, oxidative and immune stresses and disruption in energy, protein and lipid metabolisms in both male and female mussels with different mechanisms. This work suggested that the gender differences should be considered in ecotoxicoproteomics. (C) 2014 Elsevier B.V. All rights reserved.
KeywordTetrabromobisphenol a Toxicological Effects Mytilus Galloprovincialis Itraq Proteomics
Department中科院海岸带环境过程与生态修复重点实验室
Subject AreaMarine & Freshwater Biology ; Toxicology
DOI10.1016/j.aquatox.2014.09.008
Funding OrganizationMarine & Freshwater Biology ; Toxicology
Indexed BySCI
WOS KeywordBROMINATED FLAME RETARDANTS ; ESCHERICHIA-COLI ; ENVIRONMENTAL-POLLUTION ; BINDING-PROTEIN ; CELL-CYCLE ; IN-VITRO ; EXPRESSION ; TBBPA ; SEA ; APOPTOSIS
SubtypeArticle
Language英语
WOS Research AreaMarine & Freshwater Biology ; Toxicology
WOS IDWOS:000346547800004
Citation statistics
Cited Times:22[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/8686
Collection中科院海岸带环境过程与生态修复重点实验室
Corresponding AuthorWu, HF (reprint author), Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China. hfwu@yic.ac.cn
Affiliation1.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationYantai Institute of Coastal Zone Research, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Ji, Chenglong,Wu, Huifeng,Wei, Lei,et al. iTRAQ-based quantitative proteomic analyses on the gender-specific responses in mussel Mytilus galloprovincialis to tetrabromobisphenol A[J]. AQUATIC TOXICOLOGY,2014,157:30-40.
APA Ji, Chenglong,Wu, Huifeng,Wei, Lei,Zhao, Jianmin,&Wu, HF .(2014).iTRAQ-based quantitative proteomic analyses on the gender-specific responses in mussel Mytilus galloprovincialis to tetrabromobisphenol A.AQUATIC TOXICOLOGY,157,30-40.
MLA Ji, Chenglong,et al."iTRAQ-based quantitative proteomic analyses on the gender-specific responses in mussel Mytilus galloprovincialis to tetrabromobisphenol A".AQUATIC TOXICOLOGY 157(2014):30-40.
Files in This Item: Download All
File Name/Size DocType Version Access License
iTRAQ-based quantita(2729KB) 开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Ji, Chenglong]'s Articles
[Wu, Huifeng]'s Articles
[Wei, Lei]'s Articles
Baidu academic
Similar articles in Baidu academic
[Ji, Chenglong]'s Articles
[Wu, Huifeng]'s Articles
[Wei, Lei]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Ji, Chenglong]'s Articles
[Wu, Huifeng]'s Articles
[Wei, Lei]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: iTRAQ-based quantitative proteomic analyses on the gender-specific responses in mussel Mytilus galloprovincialis to tetrabromobisphenol A.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.