Institutional Repository of Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences (KLCEP)
| Combined metabolome and proteome analysis of the mantle tissue from Pacific oyster Crassostrea gigas exposed to elevated pCO(2) | |
Wei, Lei1; Wang, Qing1 ; Ning, Xuanxuan2; Mu, Changkao3; Wang, Chunlin3; Cao, Ruiwen1,4; Wu, Huifeng1; Cong, Ming1; Li, Fei1; Ji, Chenglong1; Zhao, Jianmin1; Wu, HF (reprint author), Chinese Acad Sci, YIC, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China. hfwu@yic.ac.cn; jmzhao@yic.ac.cn
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| 发表期刊 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
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| ISSN | 1744-117X |
| 2015-03-01 | |
| 卷号 | 13页码:16-23 |
| 关键词 | Ocean Acidification Proteomics Metabolomics Crassostrea Gigas |
| DOI | 10.1016/j.cbd2014.12.001 |
| 产权排序 | [Wei, Lei; Wang, Qing; Cao, Ruiwen; Wu, Huifeng; Cong, Ming; Li, Fei; Ji, Chenglong; Zhao, Jianmin] Chinese Acad Sci, YIC, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Ning, Xuanxuan] SOA, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China; [Mu, Changkao; Wang, Chunlin] Ningbo Univ, Fac Life Sci & Biotechnol, Key Lab Appl Marine Biotechnol, Minist Educ, Ningbo 315211, Zhejiang, Peoples R China; [Cao, Ruiwen] Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 作者部门 | 中科院海岸带环境过程与生态修复重点实验室 |
| 英文摘要 | Ocean acidification (OA) has been found to affect an array of normal physiological processes in mollusks, especially posing a significant threat to the fabrication process of mollusk shell. In the current study, the impact of exposure to elevated pCO(2) condition was investigated in mantle tissue of Crassostrea gigas by an integrated metabolomic and proteomic approach. Analysis of metabolome and proteome revealed that elevated pCO(2) could affect energy metabolism in oyster C. gigas, marked by differentially altered ATP, succinate, MDH, PEPCK and ALDH levels. Moreover, the up-regulated calponin-2, tropomyosins and myosin light chains indicated that elevated pCO(2) probably caused disturbances in cytoskeleton structure in mantle tissue of oyster C.gigas. This work demonstrated that a combination of proteomics and metabolomics could provide important insights into the effects of OA at molecular levels. (C) 2014 Elsevier Inc. All rights reserved.; Ocean acidification (OA) has been found to affect an array of normal physiological processes in mollusks, especially posing a significant threat to the fabrication process of mollusk shell. In the current study, the impact of exposure to elevated pCO(2) condition was investigated in mantle tissue of Crassostrea gigas by an integrated metabolomic and proteomic approach. Analysis of metabolome and proteome revealed that elevated pCO(2) could affect energy metabolism in oyster C. gigas, marked by differentially altered ATP, succinate, MDH, PEPCK and ALDH levels. Moreover, the up-regulated calponin-2, tropomyosins and myosin light chains indicated that elevated pCO(2) probably caused disturbances in cytoskeleton structure in mantle tissue of oyster C.gigas. This work demonstrated that a combination of proteomics and metabolomics could provide important insights into the effects of OA at molecular levels. (C) 2014 Elsevier Inc. All rights reserved. |
| 文章类型 | Article |
| 资助机构 | Biochemistry & Molecular Biology ; Genetics & Heredity |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | LASER-DESORPTION/IONIZATION-TIME ; CLAM RUDITAPES-PHILIPPINARUM ; EARTHWORM EISENIA-FOETIDA ; SMOOTH-MUSCLE MYOSIN ; OCEAN ACIDIFICATION ; MYTILUS-GALLOPROVINCIALIS ; SACCOSTREA-GLOMERATA ; 2,2',4,4'-TETRABROMODIPHENYL ETHER ; MALATE-DEHYDROGENASES ; METAL CONTAMINATION |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Genetics & Heredity |
| WOS记录号 | WOS:000351032100003 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/8646 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室 |
| 通讯作者 | Wu, HF (reprint author), Chinese Acad Sci, YIC, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China. hfwu@yic.ac.cn; jmzhao@yic.ac.cn |
| 作者单位 | 1.Chinese Acad Sci, YIC, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China 2.SOA, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China 3.Ningbo Univ, Fac Life Sci & Biotechnol, Key Lab Appl Marine Biotechnol, Minist Educ, Ningbo 315211, Zhejiang, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wei, Lei,Wang, Qing,Ning, Xuanxuan,et al. Combined metabolome and proteome analysis of the mantle tissue from Pacific oyster Crassostrea gigas exposed to elevated pCO(2)[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,2015,13:16-23. |
| APA | Wei, Lei.,Wang, Qing.,Ning, Xuanxuan.,Mu, Changkao.,Wang, Chunlin.,...&jmzhao@yic.ac.cn.(2015).Combined metabolome and proteome analysis of the mantle tissue from Pacific oyster Crassostrea gigas exposed to elevated pCO(2).COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,13,16-23. |
| MLA | Wei, Lei,et al."Combined metabolome and proteome analysis of the mantle tissue from Pacific oyster Crassostrea gigas exposed to elevated pCO(2)".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS 13(2015):16-23. |
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