YIC-IR  > 中科院海岸带环境过程与生态修复重点实验室
Combined metabolome and proteome analysis of the mantle tissue from Pacific oyster Crassostrea gigas exposed to elevated pCO(2)
Wei, Lei1; Wang, Qing1; Ning, Xuanxuan2; Mu, Changkao3; Wang, Chunlin3; Cao, Ruiwen1,4; Wu, Huifeng1; Cong, Ming1; Li, Fei1; Ji, Chenglong1; Zhao, Jianmin1; Wu, HF (reprint author), Chinese Acad Sci, YIC, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China. hfwu@yic.ac.cn; jmzhao@yic.ac.cn
Source PublicationCOMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
ISSN1744-117X
2015-03-01
Volume13Pages:16-23
KeywordOcean Acidification Proteomics Metabolomics Crassostrea Gigas
DOI10.1016/j.cbd2014.12.001
Contribution Rank[Wei, Lei; Wang, Qing; Cao, Ruiwen; Wu, Huifeng; Cong, Ming; Li, Fei; Ji, Chenglong; Zhao, Jianmin] Chinese Acad Sci, YIC, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Ning, Xuanxuan] SOA, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China; [Mu, Changkao; Wang, Chunlin] Ningbo Univ, Fac Life Sci & Biotechnol, Key Lab Appl Marine Biotechnol, Minist Educ, Ningbo 315211, Zhejiang, Peoples R China; [Cao, Ruiwen] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Department中科院海岸带环境过程与生态修复重点实验室
AbstractOcean acidification (OA) has been found to affect an array of normal physiological processes in mollusks, especially posing a significant threat to the fabrication process of mollusk shell. In the current study, the impact of exposure to elevated pCO(2) condition was investigated in mantle tissue of Crassostrea gigas by an integrated metabolomic and proteomic approach. Analysis of metabolome and proteome revealed that elevated pCO(2) could affect energy metabolism in oyster C. gigas, marked by differentially altered ATP, succinate, MDH, PEPCK and ALDH levels. Moreover, the up-regulated calponin-2, tropomyosins and myosin light chains indicated that elevated pCO(2) probably caused disturbances in cytoskeleton structure in mantle tissue of oyster C.gigas. This work demonstrated that a combination of proteomics and metabolomics could provide important insights into the effects of OA at molecular levels. (C) 2014 Elsevier Inc. All rights reserved.; Ocean acidification (OA) has been found to affect an array of normal physiological processes in mollusks, especially posing a significant threat to the fabrication process of mollusk shell. In the current study, the impact of exposure to elevated pCO(2) condition was investigated in mantle tissue of Crassostrea gigas by an integrated metabolomic and proteomic approach. Analysis of metabolome and proteome revealed that elevated pCO(2) could affect energy metabolism in oyster C. gigas, marked by differentially altered ATP, succinate, MDH, PEPCK and ALDH levels. Moreover, the up-regulated calponin-2, tropomyosins and myosin light chains indicated that elevated pCO(2) probably caused disturbances in cytoskeleton structure in mantle tissue of oyster C.gigas. This work demonstrated that a combination of proteomics and metabolomics could provide important insights into the effects of OA at molecular levels. (C) 2014 Elsevier Inc. All rights reserved.
SubtypeArticle
Indexed BySCI
Language英语
WOS KeywordLASER-DESORPTION/IONIZATION-TIME ; CLAM RUDITAPES-PHILIPPINARUM ; EARTHWORM EISENIA-FOETIDA ; SMOOTH-MUSCLE MYOSIN ; OCEAN ACIDIFICATION ; MYTILUS-GALLOPROVINCIALIS ; SACCOSTREA-GLOMERATA ; 2,2',4,4'-TETRABROMODIPHENYL ETHER ; MALATE-DEHYDROGENASES ; METAL CONTAMINATION
WOS Research AreaBiochemistry & Molecular Biology ; Genetics & Heredity
WOS IDWOS:000351032100003
Citation statistics
Cited Times:27[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/8646
Collection中科院海岸带环境过程与生态修复重点实验室
Corresponding AuthorWu, HF (reprint author), Chinese Acad Sci, YIC, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China. hfwu@yic.ac.cn; jmzhao@yic.ac.cn
Affiliation1.Chinese Acad Sci, YIC, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
2.SOA, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China
3.Ningbo Univ, Fac Life Sci & Biotechnol, Key Lab Appl Marine Biotechnol, Minist Educ, Ningbo 315211, Zhejiang, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Wei, Lei,Wang, Qing,Ning, Xuanxuan,et al. Combined metabolome and proteome analysis of the mantle tissue from Pacific oyster Crassostrea gigas exposed to elevated pCO(2)[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,2015,13:16-23.
APA Wei, Lei.,Wang, Qing.,Ning, Xuanxuan.,Mu, Changkao.,Wang, Chunlin.,...&jmzhao@yic.ac.cn.(2015).Combined metabolome and proteome analysis of the mantle tissue from Pacific oyster Crassostrea gigas exposed to elevated pCO(2).COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,13,16-23.
MLA Wei, Lei,et al."Combined metabolome and proteome analysis of the mantle tissue from Pacific oyster Crassostrea gigas exposed to elevated pCO(2)".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS 13(2015):16-23.
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