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Multiple biomarkers of the cytotoxicity induced by BDE-47 in human embryonic kidney cells
Wu, Huifeng1; Cao, Lulu1,2; Li, Fei1; Lian, Peiwen3; Zhao, Jianmin1; Li, F (reprint author), Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai 264003, Shandong, Peoples R China. fli@yic.ac.cn
2015-05-01
Source PublicationCHEMOSPHERE
ISSN0045-6535
Volume126Pages:32-39
Contribution Rank[Wu, Huifeng; Cao, Lulu; Li, Fei; Zhao, Jianmin] Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai 264003, Shandong, Peoples R China; [Cao, Lulu] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Lian, Peiwen] Yantai Yuhuangding Hosp, Dept Ctr Lab, Yantai 264000, Peoples R China
AbstractPolybrominated diphenyl ethers (PBDEs) are widely used as brominated flame-retardants in a variety of industrial products. Among these PBDEs, 2,2',4,4'-tetra-bromodiphenyl ether (BDE-47) is one of the most predominant congeners inducing multiple toxicities, including hepatotoxicity, neurotoxicity, cytotoxicity, genotoxicity, carcinogenecity and immunotoxicity in human body. In this study, the cytotoxicity of BDE-47 in human embryonic kidney cells (HEK293) was investigated by a set of bioassays, including cell proliferation, apoptosis, oxidative stress and metabolic responses as well as gene expressions related to apoptosis. Results showed that BDE-47 induced an inverted U-shaped curve of cell proliferation in HEK293 cells from 10(-6) to 10(-4) M. Cell apoptosis and ROS overproduction were detected at 10(-5) M of BDE-47 (p < 0.05). In addition, the expressions of Bcl-2 family-encoding genes (Bad, Hrk and Bcl-2) increased significantly in 10(-4) M group (p <0.05). Metabolic responses indicated that BDE-47 mainly caused disturbance in energy metabolism marked by differentially altered ethanol, glutathione, creatine, aspartate, UDP-glucose and NAD(+). The increased lactatefalanine ratios indicated the higher reductive state induced by BDE-47 in all exposures confirmed by the overproduction of ROS. (C) 2015 Elsevier Ltd. All rights reserved.; Polybrominated diphenyl ethers (PBDEs) are widely used as brominated flame-retardants in a variety of industrial products. Among these PBDEs, 2,2',4,4'-tetra-bromodiphenyl ether (BDE-47) is one of the most predominant congeners inducing multiple toxicities, including hepatotoxicity, neurotoxicity, cytotoxicity, genotoxicity, carcinogenecity and immunotoxicity in human body. In this study, the cytotoxicity of BDE-47 in human embryonic kidney cells (HEK293) was investigated by a set of bioassays, including cell proliferation, apoptosis, oxidative stress and metabolic responses as well as gene expressions related to apoptosis. Results showed that BDE-47 induced an inverted U-shaped curve of cell proliferation in HEK293 cells from 10(-6) to 10(-4) M. Cell apoptosis and ROS overproduction were detected at 10(-5) M of BDE-47 (p < 0.05). In addition, the expressions of Bcl-2 family-encoding genes (Bad, Hrk and Bcl-2) increased significantly in 10(-4) M group (p <0.05). Metabolic responses indicated that BDE-47 mainly caused disturbance in energy metabolism marked by differentially altered ethanol, glutathione, creatine, aspartate, UDP-glucose and NAD(+). The increased lactatefalanine ratios indicated the higher reductive state induced by BDE-47 in all exposures confirmed by the overproduction of ROS. (C) 2015 Elsevier Ltd. All rights reserved.
KeywordBde-47 Hek293 Cells Cytotoxicity Cell Apoptosis Ros Metabolomics
Department中科院海岸带环境过程与生态修复重点实验室
Subject AreaEnvironmental Sciences
DOI10.1016/j.chemosphere.2015.01.055
Funding OrganizationEnvironmental Sciences & Ecology
Indexed BySCI
WOS KeywordPOLYBROMINATED DIPHENYL ETHERS ; CLAM RUDITAPES-PHILIPPINARUM ; BROMINATED FLAME RETARDANTS ; 2,2',4,4'-TETRABROMODIPHENYL ETHER ; METABOLIC-RESPONSES ; DEVELOPMENTAL EXPOSURE ; MOLECULAR-MECHANISM ; OXIDATIVE STRESS ; NORTH CHINA ; PBDE LEVELS
SubtypeArticle
Language英语
WOS Research AreaEnvironmental Sciences & Ecology
WOS IDWOS:000351979800005
Citation statistics
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/8628
Collection中科院海岸带环境过程与生态修复重点实验室
Corresponding AuthorLi, F (reprint author), Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai 264003, Shandong, Peoples R China. fli@yic.ac.cn
Affiliation1.Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai 264003, Shandong, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Yantai Yuhuangding Hosp, Dept Ctr Lab, Yantai 264000, Peoples R China
First Author AffilicationYantai Institute of Coastal Zone Research, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wu, Huifeng,Cao, Lulu,Li, Fei,et al. Multiple biomarkers of the cytotoxicity induced by BDE-47 in human embryonic kidney cells[J]. CHEMOSPHERE,2015,126:32-39.
APA Wu, Huifeng,Cao, Lulu,Li, Fei,Lian, Peiwen,Zhao, Jianmin,&Li, F .(2015).Multiple biomarkers of the cytotoxicity induced by BDE-47 in human embryonic kidney cells.CHEMOSPHERE,126,32-39.
MLA Wu, Huifeng,et al."Multiple biomarkers of the cytotoxicity induced by BDE-47 in human embryonic kidney cells".CHEMOSPHERE 126(2015):32-39.
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