A Novel C-Type Lysozyme from Mytilus galloprovincialis: Insight into Innate Immunity and Molecular Evolution of Invertebrate C-Type Lysozymes
Wang, Qing1; Wang, Chunyan2; Mu, Changkao2; Wu, Huifeng1; Zhang, Linbao1; Zhao, Jianmin1; Wu, HF (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai, Peoples R China. hfwu@yic.ac.cn; jmzhao@yic.ac.cn
发表期刊PLOS ONE
ISSN1932-6203
2013-06-20
卷号8期号:6页码:e67469
关键词Egg-white Lysozyme Expression Analysis Drosophila-melanogaster Maximum-likelihood Positive Selection Shrimp Lysozyme Gene-expression Salivary-glands Lytic Activity Bombyx-mori
产权排序[Wang, Qing; Wu, Huifeng; Zhang, Linbao; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai, Peoples R China; [Wang, Chunyan; Mu, Changkao] Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China
英文摘要A c-type lysozyme (named as MgCLYZ) gene was cloned from the mussel Mytilus galloprovincialis. Blast analysis indicated that MgCLYZ was a salivary c-type lysozyme which was mainly found in insects. The nucleotide sequence of MgCLYZ was predicted to encode a polypeptide of 154 amino acid residues with the signal peptide comprising the first 24 residues. The deduced mature peptide of MgCLYZ was of a calculated molecular weight of 14.4 kD and a theoretical isoelectric point (pI) of 8.08. Evolution analysis suggested that bivalve branch of the invertebrate c-type lysozymes phylogeny tree underwent positive selection during evolution. By quantitative real-time RT-PCR (qRT-PCR) analysis, MgCLYZ transcript was widely detected in all examined tissues and responded sensitively to bacterial challenge in hemocytes and hepatopancreas. The optimal temperature and pH of recombinant MgCLYZ (rMgCLYZ) were 20 degrees C and 4, respectively. The rMgCLYZ displayed lytic activities against Gram-positive bacteria including Micrococcus luteus and Staphyloccocus aureus, and Gram-negative bacteria including Vibrio anguillarum, Enterobacter cloacae, Pseudomonas putida, Proteus mirabilis and Bacillus aquimaris. These results suggest that MgCLYZ perhaps play an important role in innate immunity of M. galloprovincialis, and invertebrate c-type lysozymes might be under positive selection in a species-specific manner during evolution for undergoing adaptation to different environment and diverse pathogens.; A c-type lysozyme (named as MgCLYZ) gene was cloned from the mussel Mytilus galloprovincialis. Blast analysis indicated that MgCLYZ was a salivary c-type lysozyme which was mainly found in insects. The nucleotide sequence of MgCLYZ was predicted to encode a polypeptide of 154 amino acid residues with the signal peptide comprising the first 24 residues. The deduced mature peptide of MgCLYZ was of a calculated molecular weight of 14.4 kD and a theoretical isoelectric point (pI) of 8.08. Evolution analysis suggested that bivalve branch of the invertebrate c-type lysozymes phylogeny tree underwent positive selection during evolution. By quantitative real-time RT-PCR (qRT-PCR) analysis, MgCLYZ transcript was widely detected in all examined tissues and responded sensitively to bacterial challenge in hemocytes and hepatopancreas. The optimal temperature and pH of recombinant MgCLYZ (rMgCLYZ) were 20 degrees C and 4, respectively. The rMgCLYZ displayed lytic activities against Gram-positive bacteria including Micrococcus luteus and Staphyloccocus aureus, and Gram-negative bacteria including Vibrio anguillarum, Enterobacter cloacae, Pseudomonas putida, Proteus mirabilis and Bacillus aquimaris. These results suggest that MgCLYZ perhaps play an important role in innate immunity of M. galloprovincialis, and invertebrate c-type lysozymes might be under positive selection in a species-specific manner during evolution for undergoing adaptation to different environment and diverse pathogens.
文章类型Article
资助机构NSFC [31172388]; 100 Talents Program of the Chinese Academy of Sciences; Department of Science and Technology of Yantai City [2011067]
收录类别SCI
语种英语
关键词[WOS]EGG-WHITE LYSOZYME ; EXPRESSION ANALYSIS ; DROSOPHILA-MELANOGASTER ; MAXIMUM-LIKELIHOOD ; POSITIVE SELECTION ; SHRIMP LYSOZYME ; GENE-EXPRESSION ; SALIVARY-GLANDS ; LYTIC ACTIVITY ; BOMBYX-MORI
研究领域[WOS]Science & Technology - Other Topics
WOS记录号WOS:000322342800136
引用统计
被引频次:25[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/6983
专题中国科学院海岸带环境过程与生态修复重点实验室
通讯作者Wu, HF (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai, Peoples R China. hfwu@yic.ac.cn; jmzhao@yic.ac.cn
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai, Peoples R China
2.Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China
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Wang, Qing,Wang, Chunyan,Mu, Changkao,et al. A Novel C-Type Lysozyme from Mytilus galloprovincialis: Insight into Innate Immunity and Molecular Evolution of Invertebrate C-Type Lysozymes[J]. PLOS ONE,2013,8(6):e67469.
APA Wang, Qing.,Wang, Chunyan.,Mu, Changkao.,Wu, Huifeng.,Zhang, Linbao.,...&jmzhao@yic.ac.cn.(2013).A Novel C-Type Lysozyme from Mytilus galloprovincialis: Insight into Innate Immunity and Molecular Evolution of Invertebrate C-Type Lysozymes.PLOS ONE,8(6),e67469.
MLA Wang, Qing,et al."A Novel C-Type Lysozyme from Mytilus galloprovincialis: Insight into Innate Immunity and Molecular Evolution of Invertebrate C-Type Lysozymes".PLOS ONE 8.6(2013):e67469.
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