Institutional Repository of Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences (KLCEP)
| Proteomic and metabolomic responses of clam Ruditapes philippinarum to arsenic exposure under different salinities | |
| 其他题名 | Tetra(p-tolyl)borate-Functionalized Solvent Polymeric Membrane A Facile and Sensitive Sensing Platform for Peroxidase and Peroxidase Mimetics.pdf |
Wu, Huifeng1 ; Liu, Xiaoli1,2; Zhang, Xingyan1,2; Ji, Chenglong1,2; Zhao, Jianmin1; Yu, Junbao1
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| 发表期刊 | AQUATIC TOXICOLOGY
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| ISSN | 0166-445X |
| 2013-07-15 | |
| 卷号 | 136页码:91-100 |
| 关键词 | Toxicological Effect Salinity Arsenic Ruditapes Philippinarum Proteomics Metabolomics |
| 产权排序 | [Wu, Huifeng; Liu, Xiaoli; Zhang, Xingyan; Ji, Chenglong; Zhao, Jianmin; Yu, Junbao] Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc,YICCAS, Yantai 264003, Peoples R China; [Liu, Xiaoli; Zhang, Xingyan; Ji, Chenglong] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 通讯作者 | Wu, HF (reprint author), Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc,YICCAS, Yantai 264003, Peoples R China. hfwu@yic.ac.cn |
| 英文摘要 | Arsenic (As) contamination is a severe problem in the intertidal zones of the Bohai Sea (China) with wide salinity variation. In the present study, we combined proteomics and metabolomics to characterize the differential responses of arsenic in clam Ruditapes philippinarum under different salinities (31.1, 23.3 and 15.6 psu). Both proteomic and metabolomic responses indicated that varying salinities could significantly affect the toxicological responses of clams to As. Metabolic biomarkers revealed that the environmentally relevant arsenic (20 mu g L-1) exposure induced disturbance in energy metabolism and/or osmotic regulation under different salinities, whereas protein biomarkers indicated oxidative stress, cellular injury and apoptosis and disturbance in energy metabolism. In addition, the up-regulated proteins including ATP synthase, succinyl-CoA synthetase and nucleoside diphosphate kinase were validated by related metabolites, succinate and ATP, which confirmed the disturbance in energy metabolism in clam gills at low salinity (15.6 psu). These findings provide important insights into toxicological effects of environmental contaminant at molecular levels using combined proteomics and metabolomics. (C) 2013 Elsevier B.V. All rights reserved.; Arsenic (As) contamination is a severe problem in the intertidal zones of the Bohai Sea (China) with wide salinity variation. In the present study, we combined proteomics and metabolomics to characterize the differential responses of arsenic in clam Ruditapes philippinarum under different salinities (31.1, 23.3 and 15.6 psu). Both proteomic and metabolomic responses indicated that varying salinities could significantly affect the toxicological responses of clams to As. Metabolic biomarkers revealed that the environmentally relevant arsenic (20 mu g L-1) exposure induced disturbance in energy metabolism and/or osmotic regulation under different salinities, whereas protein biomarkers indicated oxidative stress, cellular injury and apoptosis and disturbance in energy metabolism. In addition, the up-regulated proteins including ATP synthase, succinyl-CoA synthetase and nucleoside diphosphate kinase were validated by related metabolites, succinate and ATP, which confirmed the disturbance in energy metabolism in clam gills at low salinity (15.6 psu). These findings provide important insights into toxicological effects of environmental contaminant at molecular levels using combined proteomics and metabolomics. (C) 2013 Elsevier B.V. All rights reserved. |
| 文章类型 | Article |
| 资助机构 | NSFC [41106102, 21237004] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | LASER-DESORPTION/IONIZATION-TIME ; METAL CONTAMINATION ; MYTILUS-EDULIS ; PROTEIN ; NMR ; IDENTIFICATION ; BINDING ; GEL ; BIOACCUMULATION ; SPECTROSCOPY |
| 研究领域[WOS] | Marine & Freshwater Biology ; Toxicology |
| WOS记录号 | WOS:000322057200009 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/6974 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 中国科学院海岸带环境过程与生态修复重点实验室_滨海湿地实验室 |
| 作者单位 | 1.Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc,YICCAS, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Huifeng,Liu, Xiaoli,Zhang, Xingyan,et al. Proteomic and metabolomic responses of clam Ruditapes philippinarum to arsenic exposure under different salinities[J]. AQUATIC TOXICOLOGY,2013,136:91-100. |
| APA | Wu, Huifeng,Liu, Xiaoli,Zhang, Xingyan,Ji, Chenglong,Zhao, Jianmin,&Yu, Junbao.(2013).Proteomic and metabolomic responses of clam Ruditapes philippinarum to arsenic exposure under different salinities.AQUATIC TOXICOLOGY,136,91-100. |
| MLA | Wu, Huifeng,et al."Proteomic and metabolomic responses of clam Ruditapes philippinarum to arsenic exposure under different salinities".AQUATIC TOXICOLOGY 136(2013):91-100. |
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