YIC-IR  > 中科院海岸带环境过程与生态修复重点实验室
Differential metabolic responses of clam Ruditapes philippinarum to Vibrio anguillarum and Vibrio splendidus challenges
Liu, Xiaoli1; Ji, Chenglong2,3; Zhao, Jianmin2,3; Wu, Huifeng2,3
2013-12-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume35Issue:6Pages:2001-2007
Contribution Rank[Liu, Xiaoli] Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China; [Ji, Chenglong; Zhao, Jianmin; Wu, Huifeng] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Ji, Chenglong; Zhao, Jianmin; Wu, Huifeng] YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
AbstractClam Ruditapes philippinarum is one of the important marine aquaculture species in North China. However, pathogens can often cause diseases and lead to massive mortalities and economic losses of clam. In this work, we compared the metabolic responses induced by Vibrio anguillarum and Vibrio splendidus challenges towards hepatopancreas of clam using NMR-based metabolomics. Metabolic responses suggested that both V anguillarum and V splendidus induced disturbances in energy metabolism and osmotic regulation, oxidative and immune stresses with different mechanisms, as indicated by correspondingly differential metabolic biomarkers (e.g., amino acids, ATP, glucose, glycogen, taurine, betaine, choline and hypotaurine) and altered mRNA expression levels of related genes including ATP synthase, ATPase, glutathione peroxidase, heat shock protein 90, defensin and lysozyme. However, V. anguillarum caused more severe oxidative and immune stresses in clam hepatopancreas than V splendidus. Our results indicated that metabolomics could be used to elucidate the biological effects of pathogens to the marine clam R. philippinarum. (C) 2013 Elsevier Ltd. All rights reserved.; Clam Ruditapes philippinarum is one of the important marine aquaculture species in North China. However, pathogens can often cause diseases and lead to massive mortalities and economic losses of clam. In this work, we compared the metabolic responses induced by Vibrio anguillarum and Vibrio splendidus challenges towards hepatopancreas of clam using NMR-based metabolomics. Metabolic responses suggested that both V anguillarum and V splendidus induced disturbances in energy metabolism and osmotic regulation, oxidative and immune stresses with different mechanisms, as indicated by correspondingly differential metabolic biomarkers (e.g., amino acids, ATP, glucose, glycogen, taurine, betaine, choline and hypotaurine) and altered mRNA expression levels of related genes including ATP synthase, ATPase, glutathione peroxidase, heat shock protein 90, defensin and lysozyme. However, V. anguillarum caused more severe oxidative and immune stresses in clam hepatopancreas than V splendidus. Our results indicated that metabolomics could be used to elucidate the biological effects of pathogens to the marine clam R. philippinarum. (C) 2013 Elsevier Ltd. All rights reserved.
Corresponding AuthorLiu, XL (reprint author), Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China. zlylxl2009@gmail.com
KeywordMetabolomics Ruditapes Philippinarum Vibrio Anguillarum Vibrio Splendidus Hepatopancreas
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
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Indexed BySCI
WOS KeywordMYTILUS-GALLOPROVINCIALIS ; PATTERN-RECOGNITION ; NMR-SPECTROSCOPY ; CHLAMYS-FARRERI ; IMMUNE-RESPONSE ; EXPOSURE ; IDENTIFICATION ; LA(NO3)(3) ; EXPRESSION ; DISEASE
SubtypeArticle
Language英语
Funding Project中国科学院海岸带环境过程与生态修复重点实验室
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000330158800037
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Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/6929
Collection中科院海岸带环境过程与生态修复重点实验室
Affiliation1.Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
3.YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
Recommended Citation
GB/T 7714
Liu, Xiaoli,Ji, Chenglong,Zhao, Jianmin,et al. Differential metabolic responses of clam Ruditapes philippinarum to Vibrio anguillarum and Vibrio splendidus challenges[J]. FISH & SHELLFISH IMMUNOLOGY,2013,35(6):2001-2007.
APA Liu, Xiaoli,Ji, Chenglong,Zhao, Jianmin,&Wu, Huifeng.(2013).Differential metabolic responses of clam Ruditapes philippinarum to Vibrio anguillarum and Vibrio splendidus challenges.FISH & SHELLFISH IMMUNOLOGY,35(6),2001-2007.
MLA Liu, Xiaoli,et al."Differential metabolic responses of clam Ruditapes philippinarum to Vibrio anguillarum and Vibrio splendidus challenges".FISH & SHELLFISH IMMUNOLOGY 35.6(2013):2001-2007.
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