Institutional Repository of Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences (KLCEP)
| Molecular cloning and differential expression patterns of sigma and omega glutathione S-transferases from Venerupis philippinarum to heavy metals and benzo[a]pyrene exposure | |
Zhang Linbao1,2; Wu Huifeng1 ; Liu Xiaoli1,2; Chen Leilei1; Wang Qing1; Zhao Jianmin1; You Liping1,2
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| 发表期刊 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
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| ISSN | 0254-4059 |
| 2012-05-01 | |
| 卷号 | 30期号:3页码:413-423 |
| 关键词 | Venerupis Philippinarum Glutathione S-transferase Mrna Expression Heavy Metals Benzo[a]Pyrene Biomarker |
| 产权排序 | [Zhang Linbao; Wu Huifeng; Liu Xiaoli; Chen Leilei; Wang Qing; Zhao Jianmin; You Liping] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Zhang Linbao; Liu Xiaoli; You Liping] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 通讯作者 | Wu, HF (reprint author), Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China.,hfwu@yic.ac.cn ; jmzhao@yic.ac.cn |
| 作者部门 | 中科院海岸带环境过程与生态修复重点实验室 |
| 英文摘要 | Glutathione S-transferases (GSTs) are a class of enzymes that facilitate the detoxification of xenobiotics, and also play important roles in antioxidant defense. We identified two glutathione S-transferase isoforms (VpGSTS, sigma GST; VpGSTO, omega GST) from Venerupis philippinarum by RACE approaches. The open reading frames of VpGSTS and VpGSTO were of 612 bp and 729 bp, encoding 203 and 242 amino acids with an estimated molecular mass of 22.88 and 27.94 kDa, respectively. The expression profiles of VpGSTS and VpGSTO responded to heavy metals and benzo[a]pyrene (B[a]P) exposure were investigated by quantitative real-time RT-PCR. The expression of VpGSTS and VpGSTO were both rapidly up-regulated, however, they showed differential expression patterns to different toxicants. Cd displayed stronger induction of VpGSTS expression with an approximately 12-fold increase than that of VpGSTO with a maximum 6.4-fold rise. Cu exposure resulted in similar expression patterns for both VpGSTS and VpGSTO. For B[a]P exposure, the maximum induction of VpGSTO was approximately two times higher than that of VpGSTS. Altogether, these findings implied the involvement of VpGSTS and VpGSTO in host antioxidant responses, and highlighted their potential as a biomarker to Cd and B[a]P exposure.; Glutathione S-transferases (GSTs) are a class of enzymes that facilitate the detoxification of xenobiotics, and also play important roles in antioxidant defense. We identified two glutathione S-transferase isoforms (VpGSTS, sigma GST; VpGSTO, omega GST) from Venerupis philippinarum by RACE approaches. The open reading frames of VpGSTS and VpGSTO were of 612 bp and 729 bp, encoding 203 and 242 amino acids with an estimated molecular mass of 22.88 and 27.94 kDa, respectively. The expression profiles of VpGSTS and VpGSTO responded to heavy metals and benzo[a]pyrene (B[a]P) exposure were investigated by quantitative real-time RT-PCR. The expression of VpGSTS and VpGSTO were both rapidly up-regulated, however, they showed differential expression patterns to different toxicants. Cd displayed stronger induction of VpGSTS expression with an approximately 12-fold increase than that of VpGSTO with a maximum 6.4-fold rise. Cu exposure resulted in similar expression patterns for both VpGSTS and VpGSTO. For B[a]P exposure, the maximum induction of VpGSTO was approximately two times higher than that of VpGSTS. Altogether, these findings implied the involvement of VpGSTS and VpGSTO in host antioxidant responses, and highlighted their potential as a biomarker to Cd and B[a]P exposure. |
| 文章类型 | Article |
| 资助机构 | Chinese Academy of Sciences; Key Laboratory of Marine Spill Oil Identification and Damage Assessment Technology[201115]; State Oceanic Administration of China |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | OYSTER CRASSOSTREA-GIGAS ; HALIOTIS-DISCUS-DISCUS ; MESSENGER-RNA ; MYTILUS-GALLOPROVINCIALIS ; AROMATIC-HYDROCARBONS ; TIGRIOPUS-JAPONICUS ; DNA-DAMAGE ; MU-CLASS ; COPPER ; BIOMARKERS |
| 研究领域[WOS] | Marine & Freshwater Biology ; Oceanography |
| WOS记录号 | WOS:000303680400008 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/6014 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室 海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang Linbao,Wu Huifeng,Liu Xiaoli,et al. Molecular cloning and differential expression patterns of sigma and omega glutathione S-transferases from Venerupis philippinarum to heavy metals and benzo[a]pyrene exposure[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2012,30(3):413-423. |
| APA | Zhang Linbao.,Wu Huifeng.,Liu Xiaoli.,Chen Leilei.,Wang Qing.,...&You Liping.(2012).Molecular cloning and differential expression patterns of sigma and omega glutathione S-transferases from Venerupis philippinarum to heavy metals and benzo[a]pyrene exposure.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,30(3),413-423. |
| MLA | Zhang Linbao,et al."Molecular cloning and differential expression patterns of sigma and omega glutathione S-transferases from Venerupis philippinarum to heavy metals and benzo[a]pyrene exposure".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 30.3(2012):413-423. |
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