YIC-IR  > 中科院海岸带环境过程与生态修复重点实验室
Molecular cloning and differential expression patterns of sigma and omega glutathione S-transferases from Venerupis philippinarum to heavy metals and benzo[a]pyrene exposure
Zhang Linbao1,2; Wu Huifeng1; Liu Xiaoli1,2; Chen Leilei1; Wang Qing1; Zhao Jianmin1; You Liping1,2
2012-05-01
Source PublicationCHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN0254-4059
Volume30Issue:3Pages:413-423
Contribution Rank[Zhang Linbao; Wu Huifeng; Liu Xiaoli; Chen Leilei; Wang Qing; Zhao Jianmin; You Liping] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Zhang Linbao; Liu Xiaoli; You Liping] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
AbstractGlutathione S-transferases (GSTs) are a class of enzymes that facilitate the detoxification of xenobiotics, and also play important roles in antioxidant defense. We identified two glutathione S-transferase isoforms (VpGSTS, sigma GST; VpGSTO, omega GST) from Venerupis philippinarum by RACE approaches. The open reading frames of VpGSTS and VpGSTO were of 612 bp and 729 bp, encoding 203 and 242 amino acids with an estimated molecular mass of 22.88 and 27.94 kDa, respectively. The expression profiles of VpGSTS and VpGSTO responded to heavy metals and benzo[a]pyrene (B[a]P) exposure were investigated by quantitative real-time RT-PCR. The expression of VpGSTS and VpGSTO were both rapidly up-regulated, however, they showed differential expression patterns to different toxicants. Cd displayed stronger induction of VpGSTS expression with an approximately 12-fold increase than that of VpGSTO with a maximum 6.4-fold rise. Cu exposure resulted in similar expression patterns for both VpGSTS and VpGSTO. For B[a]P exposure, the maximum induction of VpGSTO was approximately two times higher than that of VpGSTS. Altogether, these findings implied the involvement of VpGSTS and VpGSTO in host antioxidant responses, and highlighted their potential as a biomarker to Cd and B[a]P exposure.; Glutathione S-transferases (GSTs) are a class of enzymes that facilitate the detoxification of xenobiotics, and also play important roles in antioxidant defense. We identified two glutathione S-transferase isoforms (VpGSTS, sigma GST; VpGSTO, omega GST) from Venerupis philippinarum by RACE approaches. The open reading frames of VpGSTS and VpGSTO were of 612 bp and 729 bp, encoding 203 and 242 amino acids with an estimated molecular mass of 22.88 and 27.94 kDa, respectively. The expression profiles of VpGSTS and VpGSTO responded to heavy metals and benzo[a]pyrene (B[a]P) exposure were investigated by quantitative real-time RT-PCR. The expression of VpGSTS and VpGSTO were both rapidly up-regulated, however, they showed differential expression patterns to different toxicants. Cd displayed stronger induction of VpGSTS expression with an approximately 12-fold increase than that of VpGSTO with a maximum 6.4-fold rise. Cu exposure resulted in similar expression patterns for both VpGSTS and VpGSTO. For B[a]P exposure, the maximum induction of VpGSTO was approximately two times higher than that of VpGSTS. Altogether, these findings implied the involvement of VpGSTS and VpGSTO in host antioxidant responses, and highlighted their potential as a biomarker to Cd and B[a]P exposure.
Corresponding AuthorWu, HF (reprint author), Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China.,hfwu@yic.ac.cn ; jmzhao@yic.ac.cn
KeywordVenerupis Philippinarum Glutathione S-transferase Mrna Expression Heavy Metals Benzo[a]Pyrene Biomarker
Department中科院海岸带环境过程与生态修复重点实验室
Subject AreaLimnology ; Oceanography
Funding OrganizationChinese Academy of Sciences; Key Laboratory of Marine Spill Oil Identification and Damage Assessment Technology[201115]; State Oceanic Administration of China
Indexed BySCI
WOS KeywordOYSTER CRASSOSTREA-GIGAS ; HALIOTIS-DISCUS-DISCUS ; MESSENGER-RNA ; MYTILUS-GALLOPROVINCIALIS ; AROMATIC-HYDROCARBONS ; TIGRIOPUS-JAPONICUS ; DNA-DAMAGE ; MU-CLASS ; COPPER ; BIOMARKERS
SubtypeArticle
Language英语
Funding ProjectZhang Linbao; Wu Huifeng; Liu Xiaoli; Chen Leilei; Wang Qing; Zhao Jianmin; You Liping
WOS Research AreaMarine & Freshwater Biology ; Oceanography
WOS IDWOS:000303680400008
Citation statistics
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/6014
Collection中科院海岸带环境过程与生态修复重点实验室
Affiliation1.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Zhang Linbao,Wu Huifeng,Liu Xiaoli,et al. Molecular cloning and differential expression patterns of sigma and omega glutathione S-transferases from Venerupis philippinarum to heavy metals and benzo[a]pyrene exposure[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2012,30(3):413-423.
APA Zhang Linbao.,Wu Huifeng.,Liu Xiaoli.,Chen Leilei.,Wang Qing.,...&You Liping.(2012).Molecular cloning and differential expression patterns of sigma and omega glutathione S-transferases from Venerupis philippinarum to heavy metals and benzo[a]pyrene exposure.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,30(3),413-423.
MLA Zhang Linbao,et al."Molecular cloning and differential expression patterns of sigma and omega glutathione S-transferases from Venerupis philippinarum to heavy metals and benzo[a]pyrene exposure".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 30.3(2012):413-423.
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