Isolation, identification and characterization of Bacillus amyloliquefaciens BZ-6, a bacterial isolate for enhancing oil recovery from oily sludge
Liu, Wuxing1; Wang, Xiaobing1; Wu, Longhua1; Chen, Mengfang1; Tu, Chen1; Luo, Yongming1,2; Christie, Peter3
2012-06-01
Source PublicationCHEMOSPHERE
ISSN0045-6535
Volume87Issue:10Pages:1105-1110
Contribution Rank[Liu, Wuxing; Wang, Xiaobing; Wu, Longhua; Chen, Mengfang; Tu, Chen; Luo, Yongming] Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China; [Luo, Yongming] Chinese Acad Sci, Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Christie, Peter] Agri Food & Biosci Inst, Agri Environm Branch, Belfast BT9 5PX, Antrim, North Ireland
AbstractOver 100 biosurfactant-producing microorganisms were isolated from oily sludge and petroleum-contaminated soil from Shengli oil field in north China. Sixteen of the bacterial isolates produced biosurfactants and reduced the surface tension of the growth medium from 71 to <30 mN m(-1) after 72 h of growth. These bacteria were used to treat oily sludge and the recovery efficiencies of oil from oily sludge were determined. The oil recovery efficiencies of different isolates ranged from 39% to 88%. Bacterial isolate BZ-6 was found to be the most efficient strain and the three phases (oil, water and sediment) were separated automatically after the sludge was treated with the culture medium of BZ-6. Based on morphological, physiological characteristics and molecular identification, isolate BZ-6 was identified as Bacillus amyloliquefaciens. The biosurfactant produced by isolate BZ-6 was purified and analyzed by high performance liquid chromatography-electrospray ionization tandem mass spectrometry. There were four ion peaks representing four different fengycin A homologues.; Over 100 biosurfactant-producing microorganisms were isolated from oily sludge and petroleum-contaminated soil from Shengli oil field in north China. Sixteen of the bacterial isolates produced biosurfactants and reduced the surface tension of the growth medium from 71 to <30 mN m(-1) after 72 h of growth. These bacteria were used to treat oily sludge and the recovery efficiencies of oil from oily sludge were determined. The oil recovery efficiencies of different isolates ranged from 39% to 88%. Bacterial isolate BZ-6 was found to be the most efficient strain and the three phases (oil, water and sediment) were separated automatically after the sludge was treated with the culture medium of BZ-6. Based on morphological, physiological characteristics and molecular identification, isolate BZ-6 was identified as Bacillus amyloliquefaciens. The biosurfactant produced by isolate BZ-6 was purified and analyzed by high performance liquid chromatography-electrospray ionization tandem mass spectrometry. There were four ion peaks representing four different fengycin A homologues. (C) 2012 Published by Elsevier Ltd.
Corresponding AuthorChristie, P (reprint author), Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China. ymluo@issas.ac.cn
KeywordOily Sludge Biosurfactants Oil Recovery Bacillus Amyloliquefaciens
Department环境化学实验室 
Subject AreaEnvironmental Sciences & Ecology
Funding OrganizationChinese Academy of Sciences[KSCX2-YW-G-053, KZCX2-YW-Q02-02]; National Natural Science Foundation of China[41001182]; Environmental Protection Public Welfare Special Fund for Scientific Research[201009015]
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Indexed BySCI
WOS KeywordBIOSURFACTANT PRODUCTION ; CONTAMINATED SOILS ; CRUDE-OIL ; MICROORGANISMS ; REMEDIATION ; SUBTILIS
SubtypeArticle
Language英语
WOS Research AreaEnvironmental Sciences & Ecology
WOS IDWOS:000303292800004
Citation statistics
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/5507
Collection中科院海岸带环境过程与生态修复重点实验室_环境化学实验室
Affiliation1.Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China
2.Chinese Acad Sci, Inst Coastal Zone Res, Yantai 264003, Peoples R China
3.Agri Food & Biosci Inst, Agri Environm Branch, Belfast BT9 5PX, Antrim, North Ireland
Recommended Citation
GB/T 7714
Liu, Wuxing,Wang, Xiaobing,Wu, Longhua,et al. Isolation, identification and characterization of Bacillus amyloliquefaciens BZ-6, a bacterial isolate for enhancing oil recovery from oily sludge[J]. CHEMOSPHERE,2012,87(10):1105-1110.
APA Liu, Wuxing.,Wang, Xiaobing.,Wu, Longhua.,Chen, Mengfang.,Tu, Chen.,...&Christie, Peter.(2012).Isolation, identification and characterization of Bacillus amyloliquefaciens BZ-6, a bacterial isolate for enhancing oil recovery from oily sludge.CHEMOSPHERE,87(10),1105-1110.
MLA Liu, Wuxing,et al."Isolation, identification and characterization of Bacillus amyloliquefaciens BZ-6, a bacterial isolate for enhancing oil recovery from oily sludge".CHEMOSPHERE 87.10(2012):1105-1110.
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