Isolation, identification and characterization of Bacillus amyloliquefaciens BZ-6, a bacterial isolate for enhancing oil recovery from oily sludge | |
Liu, Wuxing1; Wang, Xiaobing1; Wu, Longhua1; Chen, Mengfang1; Tu, Chen1; Luo, Yongming1,2; Christie, Peter3 | |
发表期刊 | CHEMOSPHERE |
ISSN | 0045-6535 |
2012-06-01 | |
卷号 | 87期号:10页码:1105-1110 |
关键词 | Oily Sludge Biosurfactants Oil Recovery Bacillus Amyloliquefaciens |
研究领域 | Environmental Sciences & Ecology |
DOI | 10.1016/j.chemosphere.2012.01.059 |
产权排序 | [Liu, Wuxing; Wang, Xiaobing; Wu, Longhua; Chen, Mengfang; Tu, Chen; Luo, Yongming] Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China; [Luo, Yongming] Chinese Acad Sci, Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Christie, Peter] Agri Food & Biosci Inst, Agri Environm Branch, Belfast BT9 5PX, Antrim, North Ireland |
作者部门 | 中科院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室 |
英文摘要 | Over 100 biosurfactant-producing microorganisms were isolated from oily sludge and petroleum-contaminated soil from Shengli oil field in north China. Sixteen of the bacterial isolates produced biosurfactants and reduced the surface tension of the growth medium from 71 to <30 mN m(-1) after 72 h of growth. These bacteria were used to treat oily sludge and the recovery efficiencies of oil from oily sludge were determined. The oil recovery efficiencies of different isolates ranged from 39% to 88%. Bacterial isolate BZ-6 was found to be the most efficient strain and the three phases (oil, water and sediment) were separated automatically after the sludge was treated with the culture medium of BZ-6. Based on morphological, physiological characteristics and molecular identification, isolate BZ-6 was identified as Bacillus amyloliquefaciens. The biosurfactant produced by isolate BZ-6 was purified and analyzed by high performance liquid chromatography-electrospray ionization tandem mass spectrometry. There were four ion peaks representing four different fengycin A homologues.;Over 100 biosurfactant-producing microorganisms were isolated from oily sludge and petroleum-contaminated soil from Shengli oil field in north China. Sixteen of the bacterial isolates produced biosurfactants and reduced the surface tension of the growth medium from 71 to <30 mN m(-1) after 72 h of growth. These bacteria were used to treat oily sludge and the recovery efficiencies of oil from oily sludge were determined. The oil recovery efficiencies of different isolates ranged from 39% to 88%. Bacterial isolate BZ-6 was found to be the most efficient strain and the three phases (oil, water and sediment) were separated automatically after the sludge was treated with the culture medium of BZ-6. Based on morphological, physiological characteristics and molecular identification, isolate BZ-6 was identified as Bacillus amyloliquefaciens. The biosurfactant produced by isolate BZ-6 was purified and analyzed by high performance liquid chromatography-electrospray ionization tandem mass spectrometry. There were four ion peaks representing four different fengycin A homologues. (C) 2012 Published by Elsevier Ltd. |
文章类型 | Article |
资助机构 | Chinese Academy of Sciences[KSCX2-YW-G-053, KZCX2-YW-Q02-02] ; National Natural Science Foundation of China[41001182] ; Environmental Protection Public Welfare Special Fund for Scientific Research[201009015] |
收录类别 | SCI |
语种 | 英语 |
关键词[WOS] | BIOSURFACTANT PRODUCTION ; CONTAMINATED SOILS ; CRUDE-OIL ; MICROORGANISMS ; REMEDIATION ; SUBTILIS |
研究领域[WOS] | Environmental Sciences & Ecology |
WOS记录号 | WOS:000303292800004 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.yic.ac.cn/handle/133337/5507 |
专题 | 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室 中国科学院海岸带环境过程与生态修复重点实验室_污染过程与控制实验室 |
通讯作者 | Luo, Yongming; Christie, Peter |
作者单位 | 1.Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China 2.Chinese Acad Sci, Inst Coastal Zone Res, Yantai 264003, Peoples R China 3.Agri Food & Biosci Inst, Agri Environm Branch, Belfast BT9 5PX, Antrim, North Ireland |
推荐引用方式 GB/T 7714 | Liu, Wuxing,Wang, Xiaobing,Wu, Longhua,et al. Isolation, identification and characterization of Bacillus amyloliquefaciens BZ-6, a bacterial isolate for enhancing oil recovery from oily sludge[J]. CHEMOSPHERE,2012,87(10):1105-1110. |
APA | Liu, Wuxing.,Wang, Xiaobing.,Wu, Longhua.,Chen, Mengfang.,Tu, Chen.,...&Christie, Peter.(2012).Isolation, identification and characterization of Bacillus amyloliquefaciens BZ-6, a bacterial isolate for enhancing oil recovery from oily sludge.CHEMOSPHERE,87(10),1105-1110. |
MLA | Liu, Wuxing,et al."Isolation, identification and characterization of Bacillus amyloliquefaciens BZ-6, a bacterial isolate for enhancing oil recovery from oily sludge".CHEMOSPHERE 87.10(2012):1105-1110. |
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