Toxicological responses to acute mercury exposure for three species of Manila clam Ruditapes philippinarum by NMR-based metabolomics
Liu, Xiaoli1,2; Zhang, Linbao1,2; You, Liping1,2; Cong, Ming1; Zhao, Jianmin1; Wu, Huifeng1; Li, Chenghua1; Liu, Dongyan1; Yu, Junbao1
Source PublicationENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
ISSN1382-6689
2011-03-01
Volume31Issue:2Pages:323-332
KeywordManila Clam Bioindicator Heavy Metal Nmr Metabolomics
Contribution Rank[Liu, Xiaoli; Zhang, Linbao; You, Liping; Cong, Ming; Zhao, Jianmin; Wu, Huifeng; Li, Chenghua; Liu, Dongyan; Yu, Junbao] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc, CAS,Shandong Prov Key Lab Coastal Zone Environm P, Yantai 264003, Shandong, Peoples R China; [Liu, Xiaoli; Zhang, Linbao; You, Liping] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
Corresponding AuthorWu, HF, Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc, CAS,Shandong Prov Key Lab Coastal Zone Environm P, 17 Chunhui Rd, Yantai 264003, Shandong, Peoples R China.hfwu@yic.ac.cn
Department污染过程与控制实验室 
AbstractThe Manila clam (Ruditapes philippinarum) has been considered a good sentinel species for metal pollution monitoring in estuarine tidal flats. Along the Bohai coast of China, there are dominantly distributed three species of clams (White, Liangdao Red and Zebra in Yantai population) endowed with distinct tolerances to environmental stressors. In this study, adductor muscle samples were collected from both control and acute mercury exposed White, Liangdao Red and Zebra clams, and the extracts were analyzed by NMR-based metabolomics to compare the metabolic profiles and responses to the acute mercury exposure to determine the most sensitive clam species capable of acting as abioindicator for heavy metal pollution monitoring. The major abundant metabolites in the White clam sample were branched-chain amino acids (leucine, isoleucine and valine), lactate, arginine, aspartate, acetylcholine, homarine and ATP/ADP, while the metabolite profile of Zebra clam sample comprised high levels of glutamine, acetoacetate, betaine, taurine and one unidentified metabolite. For the Liangdao Red clam sample, the metabolite profile relatively exhibited high amount of branched-chain amino acids, arginine, glutamate, succinate, acetylcholine, homarine and two unassigned metabolites. After 48h exposure of 20 mu g L-1 Hg2+, the metabolic profiles showed significant differences between three clam species, which included increased lactate, succinate, taurine, acetylcholine, betaine and homarine and decreased alanine, arginine, glutamine, glutamate, acetoacetate, glycine and ATP/ADP in White clam samples, and elevated succinate, taurine and acetylcholine, and declined glutamine, glycine, and aspartate in Liangdao Red clam samples, while the increased branched-chain amino acids, lactate, succinate, acetylcholine and homarine, and reduced alanine, acetoacetate, glycine and taurine were observed in the Zebra clam samples. Overall, our findings showed that White clams could be a preferable bioindicator for the metal pollution monitoring based on the more sensitive metabolic changes in the adductor muscle compared with other two (Liangdao Red and Zebra) clam species. (C) 2010 Elsevier B.V. All rights reserved.; The Manila clam (Ruditapes philippinarum) has been considered a good sentinel species for metal pollution monitoring in estuarine tidal flats. Along the Bohai coast of China, there are dominantly distributed three species of clams (White, Liangdao Red and Zebra in Yantai population) endowed with distinct tolerances to environmental stressors. In this study, adductor muscle samples were collected from both control and acute mercury exposed White, Liangdao Red and Zebra clams, and the extracts were analyzed by NMR-based metabolomics to compare the metabolic profiles and responses to the acute mercury exposure to determine the most sensitive clam species capable of acting as abioindicator for heavy metal pollution monitoring. The major abundant metabolites in the White clam sample were branched-chain amino acids (leucine, isoleucine and valine), lactate, arginine, aspartate, acetylcholine, homarine and ATP/ADP, while the metabolite profile of Zebra clam sample comprised high levels of glutamine, acetoacetate, betaine, taurine and one unidentified metabolite. For the Liangdao Red clam sample, the metabolite profile relatively exhibited high amount of branched-chain amino acids, arginine, glutamate, succinate, acetylcholine, homarine and two unassigned metabolites. After 48h exposure of 20 mu g L-1 Hg2+, the metabolic profiles showed significant differences between three clam species, which included increased lactate, succinate, taurine, acetylcholine, betaine and homarine and decreased alanine, arginine, glutamine, glutamate, acetoacetate, glycine and ATP/ADP in White clam samples, and elevated succinate, taurine and acetylcholine, and declined glutamine, glycine, and aspartate in Liangdao Red clam samples, while the increased branched-chain amino acids, lactate, succinate, acetylcholine and homarine, and reduced alanine, acetoacetate, glycine and taurine were observed in the Zebra clam samples. Overall, our findings showed that White clams could be a preferable bioindicator for the metal pollution monitoring based on the more sensitive metabolic changes in the adductor muscle compared with other two (Liangdao Red and Zebra) clam species. (C) 2010 Elsevier B.V. All rights reserved.
SubtypeArticle
Indexed BySCI
Language英语
WOS KeywordMAGNETIC-RESONANCE-SPECTROSCOPY ; PATTERN-RECOGNITION ; H-1-NMR METABOLOMICS ; TIDAL FLATS ; METABONOMICS ; EXTRACTION ; TISSUE ; PERFORMANCE ; LA(NO3)(3) ; BIOMARKER
WOS Research AreaEnvironmental Sciences & Ecology ; Pharmacology & Pharmacy ; Toxicology
WOS IDWOS:000288636200008
Citation statistics
Cited Times:54[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/4909
Collection中科院海岸带环境过程与生态修复重点实验室_污染过程与控制实验室
中科院海岸带环境过程与生态修复重点实验室
Affiliation1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc, CAS,Shandong Prov Key Lab Coastal Zone Environm P, Yantai 264003, Shandong, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Liu, Xiaoli,Zhang, Linbao,You, Liping,et al. Toxicological responses to acute mercury exposure for three species of Manila clam Ruditapes philippinarum by NMR-based metabolomics[J]. ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY,2011,31(2):323-332.
APA Liu, Xiaoli.,Zhang, Linbao.,You, Liping.,Cong, Ming.,Zhao, Jianmin.,...&Yu, Junbao.(2011).Toxicological responses to acute mercury exposure for three species of Manila clam Ruditapes philippinarum by NMR-based metabolomics.ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY,31(2),323-332.
MLA Liu, Xiaoli,et al."Toxicological responses to acute mercury exposure for three species of Manila clam Ruditapes philippinarum by NMR-based metabolomics".ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY 31.2(2011):323-332.
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