The present invention discloses a fluorescent probe for detecting hydrogen polysulfide and its preparation method and application. wherein the fluorescent probe is an organic small molecule compound based on a coumarin derivative, The structural formula of the fluorescent probe is shown in the specification. The fluorescent probe can respond to H2Sn with high selectivity and high sensitivity. In the presence of H2Sn, a fluorescent spectrum has an obvious and new emission peak. The fluorescent probe can be used for qualitative and quantitative detection of H2Sn in solution and cells, can greatly reduce interference of external detection conditions, and has high detection speed and high detection precision.
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