The lysosome-phagosome pathway mediates immune regulatory mechanisms in Mesocentrotus nudus against Vibrio coralliilyticus infection

doi:10.1016/j.fsi.2023.108864

	The lysosome-phagosome pathway mediates immune regulatory mechanisms in Mesocentrotus nudus against Vibrio coralliilyticus infection
	Wang, Yanxia 1,2; Wang, Quanchao 1,3; Chen, Linlin1,3 ; Li, Baoquan1,3,4
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
	2023-08-01
卷号	139 页码:13
关键词	Mesocentrotusnudus Lysosome Phagosome Vibrio coralliilyticus V-ATPases
DOI	10.1016/j.fsi.2023.108864
通讯作者	Li, Baoquan(bqli@yic.ac.cn)
英文摘要	Sea urchins are a popular model species for studying invertebrate diseases. The immune regulatory mechanisms of the sea urchin Mesocentrotus nudus during pathogenic infection are currently unknown. This study aimed to reveal the potential molecular mechanisms of M. nudus during resistance to Vibrio coralliilyticus infection by integrative transcriptomic and proteomic analyses. Here, we identified a total of 135,868 unigenes and 4,351 proteins in the four infection periods of 0 h, 20 h, 60 h and 100 h in M. nudus. In the I20, I60 and I100 infection comparison groups, 10,861, 15,201 and 8,809 differentially expressed genes (DEGs) and 2,188, 2,386 and 2,516 differentially expressed proteins (DEPs) were identified, respectively. We performed an integrated comparative analysis of the transcriptome and proteome throughout the infection phase and found very a low correlation between transcriptome and proteome changes. KEGG pathway analysis revealed that most upregulated DEGs and DEPs were involved in immune strategies. Notably, "lysosome" and "phagosome" activated throughout the infection process, could be considered the two most important enrichment pathways at the mRNA and protein levels. The significant increase in phagocytosis of infected M. nudus coelomocytes further demonstrated that the lysosome-phagosome pathway played an important immunological role in M. nudus resistance to pathogenic infection. Key gene expression profiles and protein-protein interaction analysis revealed that cathepsin family and V-ATPase family genes might be key bridges in the lysosome-phagosome pathway. In addition, the expression patterns of key immune genes were verified using qRT-PCR, and the different expression trends of candidate genes reflected, to some extent, the regulatory mechanism of immune homeostasis mediated by the lysosome-phagosome pathway in M. nudus against pathogenic infection. This work will provide new insights into the immune regulatory mechanisms of sea urchins under pathogenic stress and help identify key potential genes/ proteins for sea urchin immune responses.
资助机构	International Partnership Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China
收录类别	SCI
语种	英语
关键词[WOS]	URCHIN STRONGYLOCENTROTUS-INTERMEDIUS ; CUCUMBER APOSTICHOPUS-JAPONICUS ; SEA ; TRANSCRIPTOME ; BACTERIA ; GENES ; PHAGOCYTOSIS ; TEMPERATURE ; MACROPHAGES ; CELOMOCYTES
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:001012377400001
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/33328
专题	中国科学院海岸带环境过程与生态修复重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室海岸带生物学与生物资源利用重点实验室
通讯作者	Li, Baoquan
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 2.Univ Chinese Acad Sci, Beijing 10049, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yanxia,Wang, Quanchao,Chen, Linlin,et al. The lysosome-phagosome pathway mediates immune regulatory mechanisms in Mesocentrotus nudus against Vibrio coralliilyticus infection[J]. FISH & SHELLFISH IMMUNOLOGY,2023,139:13.
APA	Wang, Yanxia,Wang, Quanchao,Chen, Linlin,&Li, Baoquan.(2023).The lysosome-phagosome pathway mediates immune regulatory mechanisms in Mesocentrotus nudus against Vibrio coralliilyticus infection.FISH & SHELLFISH IMMUNOLOGY,139,13.
MLA	Wang, Yanxia,et al."The lysosome-phagosome pathway mediates immune regulatory mechanisms in Mesocentrotus nudus against Vibrio coralliilyticus infection".FISH & SHELLFISH IMMUNOLOGY 139(2023):13.