Construction of a myoglobin scaffold-based biocatalyst for the biodegradation of sulfadiazine and sulfathiazole
Zhang, Weikang1,2; Lin, Yingwu3; Meng, Xiangmin2; Wang, Qiaoning4; Chen, Lingxin4; Xu, Jiakun1
Source PublicationJOURNAL OF HAZARDOUS MATERIALS
ISSN0304-3894
2024-03-05
Volume465Pages:10
KeywordSulfonamides Myoglobin Biocatalysis Biodegradation Directed evolution
DOI10.1016/j.jhazmat.2023.133147
Corresponding AuthorChen, Lingxin(lxchen@yic.ac.cn) ; Xu, Jiakun(xujk@ysfri.ac.cn)
AbstractSulfonamide antibiotics, a family of broad-spectrum antibiotic drugs, are increasingly used in aquaculture and are frequently detected in aquatic environments. This poses a potential threat to organisms and may cause the evolution of antimicrobial resistance. Therefore, it is important to develop an environmentally friendly and efficient biocatalyst to degrade sulfonamides (SAs) such as sulfadiazine (SD) and sulfathiazole (ST). Here, we realized the direct and efficient degradation of SD and ST using a hydrogen peroxide-dependent artificial catalytic system based on myoglobin (Mb). The arrangements of amino acids at positions 29, 43, 64, and 68 were found to influence catalytic activity. An L29H/H64D/V68I myoglobin mutant showed the best catalytic efficiency (i.e., kcat/Km = 720.42 M-1 s-1) against SD. Next, mutant H64D/V68I showed the best degradation rate against SD (i.e., 91.45 +/- 0.16%). Moreover, L29H/H64D/V68I Mb was found to efficiently catalyze ST oxidation
Funding OrganizationNational Key R & D Program of China ; National Natural Science Foundation of China ; Central Public-Interest Scientific Institution Basal Research Fund, CAFS
Indexed BySCI
Language英语
WOS KeywordCOMPOUND-I ; ANTIBIOTICS ; SULFAMETHOXAZOLE ; SULFONAMIDES ; KINETICS ; BIOTRANSFORMATION ; SULFAMETHAZINE ; TRANSFORMATION ; DEGRADATION ; ENVIRONMENT
WOS Research AreaEngineering ; Environmental Sciences & Ecology
WOS IDWOS:001134426400001
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/33118
Collection中国科学院海岸带环境过程与生态修复重点实验室
中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
Corresponding AuthorChen, Lingxin; Xu, Jiakun
Affiliation1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, State Key Lab Mariculture Biobreeding & Sustainabl, Lab Marine Drugs & Byprod Pilot Natl Lab Marine Sc, Qingdao 266071, Peoples R China
2.Qingdao Univ Sci & Technol, Coll Marine Sci & Biol Engn, Qingdao 266042, Peoples R China
3.Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China
4.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Weikang,Lin, Yingwu,Meng, Xiangmin,et al. Construction of a myoglobin scaffold-based biocatalyst for the biodegradation of sulfadiazine and sulfathiazole[J]. JOURNAL OF HAZARDOUS MATERIALS,2024,465:10.
APA Zhang, Weikang,Lin, Yingwu,Meng, Xiangmin,Wang, Qiaoning,Chen, Lingxin,&Xu, Jiakun.(2024).Construction of a myoglobin scaffold-based biocatalyst for the biodegradation of sulfadiazine and sulfathiazole.JOURNAL OF HAZARDOUS MATERIALS,465,10.
MLA Zhang, Weikang,et al."Construction of a myoglobin scaffold-based biocatalyst for the biodegradation of sulfadiazine and sulfathiazole".JOURNAL OF HAZARDOUS MATERIALS 465(2024):10.
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