Construction of a myoglobin scaffold-based biocatalyst for the biodegradation of sulfadiazine and sulfathiazole

doi:10.1016/j.jhazmat.2023.133147

	Construction of a myoglobin scaffold-based biocatalyst for the biodegradation of sulfadiazine and sulfathiazole
	Zhang, Weikang 1,2; Lin, Yingwu 3; Meng, Xiangmin 2; Wang, Qiaoning 4; Chen, Lingxin4 ; Xu, Jiakun 1
发表期刊	JOURNAL OF HAZARDOUS MATERIALS
ISSN	0304-3894
	2024-03-05
卷号	465 页码:10
关键词	Sulfonamides Myoglobin Biocatalysis Biodegradation Directed evolution
DOI	10.1016/j.jhazmat.2023.133147
通讯作者	Chen, Lingxin(lxchen@yic.ac.cn) ; Xu, Jiakun(xujk@ysfri.ac.cn)
英文摘要	Sulfonamide antibiotics, a family of broad-spectrum antibiotic drugs, are increasingly used in aquaculture and are frequently detected in aquatic environments. This poses a potential threat to organisms and may cause the evolution of antimicrobial resistance. Therefore, it is important to develop an environmentally friendly and efficient biocatalyst to degrade sulfonamides (SAs) such as sulfadiazine (SD) and sulfathiazole (ST). Here, we realized the direct and efficient degradation of SD and ST using a hydrogen peroxide-dependent artificial catalytic system based on myoglobin (Mb). The arrangements of amino acids at positions 29, 43, 64, and 68 were found to influence catalytic activity. An L29H/H64D/V68I myoglobin mutant showed the best catalytic efficiency (i.e., kcat/Km = 720.42 M-1 s-1) against SD. Next, mutant H64D/V68I showed the best degradation rate against SD (i.e., 91.45 +/- 0.16%). Moreover, L29H/H64D/V68I Mb was found to efficiently catalyze ST oxidation
资助机构	National Key R & D Program of China ; National Natural Science Foundation of China ; Central Public-Interest Scientific Institution Basal Research Fund, CAFS
收录类别	SCI
语种	英语
关键词[WOS]	COMPOUND-I ; ANTIBIOTICS ; SULFAMETHOXAZOLE ; SULFONAMIDES ; KINETICS ; BIOTRANSFORMATION ; SULFAMETHAZINE ; TRANSFORMATION ; DEGRADATION ; ENVIRONMENT
研究领域[WOS]	Engineering ; Environmental Sciences & Ecology
WOS记录号	WOS:001134426400001
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/33118
专题	中国科学院海岸带环境过程与生态修复重点实验室中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
通讯作者	Chen, Lingxin; Xu, Jiakun
作者单位	1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, State Key Lab Mariculture Biobreeding & Sustainabl, Lab Marine Drugs & Byprod Pilot Natl Lab Marine Sc, Qingdao 266071, Peoples R China 2.Qingdao Univ Sci & Technol, Coll Marine Sci & Biol Engn, Qingdao 266042, Peoples R China 3.Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China 4.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Weikang,Lin, Yingwu,Meng, Xiangmin,et al. Construction of a myoglobin scaffold-based biocatalyst for the biodegradation of sulfadiazine and sulfathiazole[J]. JOURNAL OF HAZARDOUS MATERIALS,2024,465:10.
APA	Zhang, Weikang,Lin, Yingwu,Meng, Xiangmin,Wang, Qiaoning,Chen, Lingxin,&Xu, Jiakun.(2024).Construction of a myoglobin scaffold-based biocatalyst for the biodegradation of sulfadiazine and sulfathiazole.JOURNAL OF HAZARDOUS MATERIALS,465,10.
MLA	Zhang, Weikang,et al."Construction of a myoglobin scaffold-based biocatalyst for the biodegradation of sulfadiazine and sulfathiazole".JOURNAL OF HAZARDOUS MATERIALS 465(2024):10.