Combined toxic effects of nanoplastics and norfloxacin on mussel: Leveraging biochemical parameters and gut microbiota
Xu, Ran1,2; Li, Li'ang1,2; Zheng, Jiahui1,2; Ji, Chenglong3; Wu, Huifeng3; Chen, Xiang1,2; Chen, Yuchuan1,2; Hu, Menghong1,2; Xu, Elvis Genbo4; Wang, Youji1,2
Source PublicationSCIENCE OF THE TOTAL ENVIRONMENT
ISSN0048-9697
2023-07-01
Volume880Pages:9
KeywordMytilus coruscus Antibiotic Biomarkers16S rRNA sequencing Joint toxicity
DOI10.1016/j.scitotenv.2023.163304
Corresponding AuthorXu, Elvis Genbo(elvis@biology.sdu.dk) ; Wang, Youji(youjiwang2@gmail.com)
AbstractAntibiotics and nanoplastics (NPs) are among the two most concerned and studied marine emerging contaminants in recent years. Given the large number of different types of antibiotics and NPs, there is a need to apply efficient tools to evaluate their combined toxic effects. Using the thick-shelled mussel (Mytilus coruscus) as a marine ecotoxicological model, we applied a battery of fast enzymatic activity assays and 16S rRNA sequencing to investigate the biochemical and gut microbial response of mussels exposed to antibiotic norfloxacin (NOR) and NPs (80 nm polystyrene beads) alone and in combination at environmentally relevant concentrations. After 15 days of exposure, NPs alone signifi-cantly inhibited superoxide dismutase (SOD) and amylase (AMS) activities, while catalase (CAT) was affected by both NOR and NPs. The changes in lysozyme (LZM) and lipase (LPS) were increased over time during the treatments. Co-exposure to NPs and NOR significantly affected glutathione (GSH) and trypsin (Typ), which might be explained by the increased bioavailable NOR carried by NPs. The richness and diversity of the gut microbiota of mussels were both decreased by exposures to NOR and NPs, and the top functions of gut microbiota that were affected by the exposures were predicted. The data fast generated by enzymatic test and 16S sequencing allowed further variance and correla-tion analysis to understand the plausible driving factors and toxicity mechanisms. Despite the toxic effects of only one type of antibiotics and NPs being evaluated, the validated assays on mussels are readily applicable to other anti-biotics, NPs, and their mixture.
Funding OrganizationCAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, YICCAS ; Innovation Program of Shanghai Municipal Education Commission ; Department of Biology, University of Southern Denmark ; Danmarks Frie Forskningsfond
Indexed BySCI
Language英语
WOS KeywordOXIDATIVE STRESS ; ANTIBIOTICS ; WATER ; MICROPLASTICS ; SEA ; ENVIRONMENTS ; WASTEWATERS ; SEWAGE
WOS Research AreaEnvironmental Sciences & Ecology
WOS IDWOS:000981809500001
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/32921
Collection中国科学院海岸带环境过程与生态修复重点实验室
中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室
Corresponding AuthorXu, Elvis Genbo; Wang, Youji
Affiliation1.Shanghai Ocean Univ, Minist Sci & Technol, Coll Fisheries & Life Sci, Int Res Ctr Marine Biosci, Shanghai 201306, Peoples R China
2.Shanghai Ocean Univ, Minist Educ, Key Lab Explorat & Utilizat Aquat Genet Resources, Shanghai 201306, Peoples R China
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Key Lab Coastal Environm Proc, Yantai 264003, Peoples R China
4.Univ Southern Denmark, Dept Biol, DK-5230 Odense M, Denmark
Recommended Citation
GB/T 7714
Xu, Ran,Li, Li'ang,Zheng, Jiahui,et al. Combined toxic effects of nanoplastics and norfloxacin on mussel: Leveraging biochemical parameters and gut microbiota[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2023,880:9.
APA Xu, Ran.,Li, Li'ang.,Zheng, Jiahui.,Ji, Chenglong.,Wu, Huifeng.,...&Wang, Youji.(2023).Combined toxic effects of nanoplastics and norfloxacin on mussel: Leveraging biochemical parameters and gut microbiota.SCIENCE OF THE TOTAL ENVIRONMENT,880,9.
MLA Xu, Ran,et al."Combined toxic effects of nanoplastics and norfloxacin on mussel: Leveraging biochemical parameters and gut microbiota".SCIENCE OF THE TOTAL ENVIRONMENT 880(2023):9.
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