Methylobacter couples methane oxidation and N2O production in hypoxic wetland soil

doi:10.1016/j.soilbio.2022.108863

	Methylobacter couples methane oxidation and N2O production in hypoxic wetland soil
	Hao, Qinqin 1,3; Wang, Oumei 1,2; Jiao, Jian-Yu 4; Xiao, Leilei5 ; Zhang, Yuezhi 2; Li, Wen-Jun4 ; Liu, Fanghua1,2,3,6
发表期刊	SOIL BIOLOGY & BIOCHEMISTRY
ISSN	0038-0717
	2022-12-01
卷号	175 页码:12
关键词	Methanotroph Methylobacter Nitrite reduction Nitrous oxide Wetland soil
DOI	10.1016/j.soilbio.2022.108863
通讯作者	Liu, Fanghua(fhliu@soil.gd.cn)
英文摘要	Aerobic methanotrophs differ in metabolic mechanisms under hypoxic environments. Methane oxidation com-bined with nitrate/nitrite reduction is related to the emissions of greenhouse gases methane and nitrous oxide (N2O). However, the mechanisms underlying methane oxidation and N2O production remain to be elucidated through environmental and pure culture experiments. Here, microcosmic experiments of inhibition of methane oxidation and isotope tracing revealed that methane oxidation coupled to N2O production occurred in hypoxic wetland soil. RNA sequence analysis showed that Methylobacter was the dominant methane oxidizer, and a novel strain Methylobacter sp. YRD-M1 was isolated. Genomic and pure cultured results suggested that nitrite was the electron acceptor of the isolated strain under hypoxic conditions with N2O production. Despite lacking the ge-netic potential for dissimilatory nitrate reduction, assimilatory nitrate reductase may be involved in incomplete denitrification, leading to N2O production. In addition, strain YRD-M1 consumed dissolved oxygen to below 10 mu M, showing a strong oxygen affinity. Furthermore, fermentation was induced with acetate and succinate released during hypoxic incubation. The strain YRD-M1 showed flexible and versatile metabolic processes in oxygen-limited systems. Notably, Methylobacter-like methanotrophs were widely distributed in terrestrial envi-ronments. Overall, our findings show that Methylobacter couples methane oxidation and N2O production in hypoxic environments from soil to pure culture, highlighting the potential role of related microbes in linking methane sinks and N2O sources in oxygen-limited environments.
资助机构	National Key Research and Development Program of China ; National Natural Science Foundation of China ; China Post-doctoral Science Foundation ; Guangdong Foundation for Program of Science and Technology Research ; Guangdong Basic and Applied Basic Research Foundation ; GDAS' Project of Science and Technology Development ; Pearl River Talent Recruitment Program of Guangdong Province
收录类别	SCI
语种	英语
关键词[WOS]	AEROBIC METHANOTROPHS ; ACETATE ; EMISSIONS ; PATHWAYS ; QUANTIFICATION ; METHANOGENESIS ; ALIGNMENT
研究领域[WOS]	Agriculture
WOS记录号	WOS:000884832900004
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/32386
专题	中国科学院海岸带环境过程与生态修复重点实验室中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室
通讯作者	Liu, Fanghua
作者单位	1.Guangdong Acad Sci, Inst Ecoenvironm & Soil Sci, Guangdong Key Lab Integrated Agroenvironm Pollut C, Natl Reg Joint Engn Res Ctr Soil Pollut Control &, Guangzhou 510650, Peoples R China 2.Binzhou Med Univ, Dept Biochem & Mol Biol, Yantai 264003, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 4.Sun Yat Sen Univ, Sch Life Sci, Guangdong Prov Key Lab Plant Resources & Southern, State Key Lab Biocontrol,Guangdong Lab Zhuhai, Guangzhou 510275, Peoples R China 5.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 6.Guangdong Acad Sci, Inst Ecoenvironm & Soil Sci, 808 Tianyuan Rd, Guangzhou 510650, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Hao, Qinqin,Wang, Oumei,Jiao, Jian-Yu,et al. Methylobacter couples methane oxidation and N2O production in hypoxic wetland soil[J]. SOIL BIOLOGY & BIOCHEMISTRY,2022,175:12.
APA	Hao, Qinqin.,Wang, Oumei.,Jiao, Jian-Yu.,Xiao, Leilei.,Zhang, Yuezhi.,...&Liu, Fanghua.(2022).Methylobacter couples methane oxidation and N2O production in hypoxic wetland soil.SOIL BIOLOGY & BIOCHEMISTRY,175,12.
MLA	Hao, Qinqin,et al."Methylobacter couples methane oxidation and N2O production in hypoxic wetland soil".SOIL BIOLOGY & BIOCHEMISTRY 175(2022):12.