Fluorescence imaging to probe mercury induced oxidative stress in living systems

doi:10.1016/j.snb.2022.131982

	Fluorescence imaging to probe mercury induced oxidative stress in living systems
	Zhang, Xia 1,5; Zhang, Li 1,5; Cheng, Xinyan 2; Liu, Shudi 2; Fang, Shujing 2; Zhang, Liangwei1,3 ; Wang, Xiaoyan 6; Chen, Lingxin1,3,4
发表期刊	SENSORS AND ACTUATORS B-CHEMICAL
	2022-09-01
卷号	366 页码:8
关键词	Fluorescent imaging Mercury stress Oxidative stress Hydrogen peroxide Hypoxia
DOI	10.1016/j.snb.2022.131982
通讯作者	Zhang, Liangwei(liangweizhang@yic.ac.cn) ; Chen, Lingxin(lxchen@yic.ac.cn)
英文摘要	Mercury is known a toxic heavy metal pollutant, and mercuric derivatives severely threaten ecological environment and human health. Their toxicological effects are usually associated with oxidative stress and redox imbalance. However, rare visualized evidences to reveal mercury induced oxidative stress in living systems. According to its characteristics, we suspected mercury resulted in two consequences, one is reactive oxygen species (ROS) outburst, the other is oxygen consumption caused hypoxia stress. Hydrogen peroxide (H2O2) is an important oxygen metabolite, and superfluous H2O2 will attack intracellular antioxidants and trigger oxidative stress, then cause dysfunctions and many diseases. Although the research of H2O2 fluorescence probe has been greatly advanced, the exploration of H2O2 bioeffects under environmental stress was scarce. There is still necessary to develop powerful tools to explore redox homeostasis and H2O2 fluctuations under mercury stress. Herein, we have designed fluorescent probe MSO-Bindol for monitoring H2O2 fluctuations through destruction of carbon-carbon double bond leading to ratiometric fluorescent changes. MSO-Bindol is successfully used to observe H2O2 variations in cells and zebrafish exposed to various stimulations or environmental stress especially mercury stress. Moreover, imaging results provide visualization proofs for Hg2+-mediated H2O2 variations and reveal the relationship between Hg2+-induced oxidative stress and up-regulated H2O2 in biological systems.
资助机构	National Natural Science Foundation of China ; Program of Youth Innovation Promotion Association, CAS ; Taishan Scholar Project Special Funding
收录类别	SCI
语种	英语
关键词[WOS]	HYDROGEN-PEROXIDE ; REACTIVE OXYGEN ; H2O2 ; MITOCHONDRIA ; SIGNALS ; CELLS
研究领域[WOS]	Chemistry ; Electrochemistry ; Instruments & Instrumentation
WOS记录号	WOS:000800383700004
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/31120
专题	中国科学院海岸带环境过程与生态修复重点实验室中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
通讯作者	Zhang, Liangwei; Chen, Lingxin
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Key Lab Coastal Environm Proc, Yantai 264003, Peoples R China 2.Yantai Univ, Coll Chem & Chem Engn, Yantai 264005, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 4.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 6.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Xia,Zhang, Li,Cheng, Xinyan,et al. Fluorescence imaging to probe mercury induced oxidative stress in living systems[J]. SENSORS AND ACTUATORS B-CHEMICAL,2022,366:8.
APA	Zhang, Xia.,Zhang, Li.,Cheng, Xinyan.,Liu, Shudi.,Fang, Shujing.,...&Chen, Lingxin.(2022).Fluorescence imaging to probe mercury induced oxidative stress in living systems.SENSORS AND ACTUATORS B-CHEMICAL,366,8.
MLA	Zhang, Xia,et al."Fluorescence imaging to probe mercury induced oxidative stress in living systems".SENSORS AND ACTUATORS B-CHEMICAL 366(2022):8.