Institutional Repository of Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences (KLCEP)
| Toxicological effects of tris(1,3-dichloro-2-propyl) phosphate in oyster Crassostrea gigas using proteomic and phosphoproteomic analyses | |
Yin, Chengcheng1,3; Sun, Zuodeng4; Ji, Chenglong1,2,5 ; Li, Fei1,5; Wu, Huifeng1,2,5
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| Source Publication | JOURNAL OF HAZARDOUS MATERIALS
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| ISSN | 0304-3894 |
| 2022-07-15 | |
| Volume | 434Pages:12 |
| Keyword | Tris(1 3-dichloro-2-propyl) phosphate Crassostrea gigas Proteomics Phosphoproteomics |
| DOI | 10.1016/j.jhazmat.2022.128824 |
| Abstract | As a typical organophosphorus pollutant, tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) has been widely detected in aquatic environment. Previous studies showed that protein phosphorylation might be a vital way of TDCIPP to exert multiple toxic effects. However, there is a lack of high-throughput investigations on how TDCIPP affected protein phosphorylation. In this study, the toxicological effects of TDCIPP were explored by proteomic and phosphoproteomic analyses together with traditional means in oysters Crassostrea gigas treated with 0.5, 5 and 50 mu g/L TDCIPP for 28 days. Integration of omic analyses revealed that TDCIPP dysregulated transcription, energy metabolism, and apoptosis and cell proliferation by either directly phosphorylating pivotal proteins or phosphorylating their upstream signaling pathways. The U-shaped response of acetylcholinesterase activities suggested the neurotoxicity of TDCIPP in a hormesis manner. What's more, the increase in caspase-9 activity as well as the expression or phosphorylation alterations in eukaryotic translation initiation factor 4E, cell division control protein 42 and transforming growth factor-beta 1-induced protein indicated the disruption of homeostasis between apoptosis and cell proliferation, which was consistent with the observation of shedding of digestive cells. Overall, combination of proteomic and phosphoproteomic analyses showed the capability of identifying molecular events, which provided new insights into the toxicological mechanisms of TDCIPP. |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | ORGANOPHOSPHORUS FLAME RETARDANTS ; QUANTITATIVE ALTERATIONS ; PROSTATE-CANCER ; CELL ; PHOSPHORYLATION ; METABOLISM ; EXPOSURE ; GROWTH ; BIOCONCENTRATION ; PROLIFERATION |
| WOS Research Area | Engineering ; Environmental Sciences & Ecology |
| WOS ID | WOS:000793533900002 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.yic.ac.cn/handle/133337/31093 |
| Collection | 中科院海岸带环境过程与生态修复重点实验室 中科院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室 |
| Corresponding Author | Ji, Chenglong; Wu, Huifeng |
| Affiliation | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Key Lab Coastal Environm Proc,YICCAS, Yantai 264003, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Shandong Fisheries Dev & Resource Conservat Ctr, Jinan 250013, Peoples R China 5.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China |
| Recommended Citation GB/T 7714 | Yin, Chengcheng,Sun, Zuodeng,Ji, Chenglong,et al. Toxicological effects of tris(1,3-dichloro-2-propyl) phosphate in oyster Crassostrea gigas using proteomic and phosphoproteomic analyses[J]. JOURNAL OF HAZARDOUS MATERIALS,2022,434:12. |
| APA | Yin, Chengcheng,Sun, Zuodeng,Ji, Chenglong,Li, Fei,&Wu, Huifeng.(2022).Toxicological effects of tris(1,3-dichloro-2-propyl) phosphate in oyster Crassostrea gigas using proteomic and phosphoproteomic analyses.JOURNAL OF HAZARDOUS MATERIALS,434,12. |
| MLA | Yin, Chengcheng,et al."Toxicological effects of tris(1,3-dichloro-2-propyl) phosphate in oyster Crassostrea gigas using proteomic and phosphoproteomic analyses".JOURNAL OF HAZARDOUS MATERIALS 434(2022):12. |
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