Toxicological effects of tris(1,3-dichloro-2-propyl) phosphate in oyster Crassostrea gigas using proteomic and phosphoproteomic analyses
Yin, Chengcheng1,3; Sun, Zuodeng4; Ji, Chenglong1,2,5; Li, Fei1,5; Wu, Huifeng1,2,5
Source PublicationJOURNAL OF HAZARDOUS MATERIALS
ISSN0304-3894
2022-07-15
Volume434Pages:12
KeywordTris(1 3-dichloro-2-propyl) phosphate Crassostrea gigas Proteomics Phosphoproteomics
DOI10.1016/j.jhazmat.2022.128824
Corresponding AuthorJi, Chenglong(clji@yic.ac.cn) ; Wu, Huifeng(hfwu@yic.ac.cn)
AbstractAs a typical organophosphorus pollutant, tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) has been widely detected in aquatic environment. Previous studies showed that protein phosphorylation might be a vital way of TDCIPP to exert multiple toxic effects. However, there is a lack of high-throughput investigations on how TDCIPP affected protein phosphorylation. In this study, the toxicological effects of TDCIPP were explored by proteomic and phosphoproteomic analyses together with traditional means in oysters Crassostrea gigas treated with 0.5, 5 and 50 mu g/L TDCIPP for 28 days. Integration of omic analyses revealed that TDCIPP dysregulated transcription, energy metabolism, and apoptosis and cell proliferation by either directly phosphorylating pivotal proteins or phosphorylating their upstream signaling pathways. The U-shaped response of acetylcholinesterase activities suggested the neurotoxicity of TDCIPP in a hormesis manner. What's more, the increase in caspase-9 activity as well as the expression or phosphorylation alterations in eukaryotic translation initiation factor 4E, cell division control protein 42 and transforming growth factor-beta 1-induced protein indicated the disruption of homeostasis between apoptosis and cell proliferation, which was consistent with the observation of shedding of digestive cells. Overall, combination of proteomic and phosphoproteomic analyses showed the capability of identifying molecular events, which provided new insights into the toxicological mechanisms of TDCIPP.
Funding OrganizationNational Natural Science Foundation of China ; Yantai Science and Technology Development Plan
Indexed BySCI
Language英语
WOS KeywordORGANOPHOSPHORUS FLAME RETARDANTS ; QUANTITATIVE ALTERATIONS ; PROSTATE-CANCER ; CELL ; PHOSPHORYLATION ; METABOLISM ; EXPOSURE ; GROWTH ; BIOCONCENTRATION ; PROLIFERATION
WOS Research AreaEngineering ; Environmental Sciences & Ecology
WOS IDWOS:000793533900002
Citation statistics
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/31093
Collection中国科学院海岸带环境过程与生态修复重点实验室
中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室
Corresponding AuthorJi, Chenglong; Wu, Huifeng
Affiliation1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Key Lab Coastal Environm Proc,YICCAS, Yantai 264003, Peoples R China
2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Shandong Fisheries Dev & Resource Conservat Ctr, Jinan 250013, Peoples R China
5.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China
Recommended Citation
GB/T 7714
Yin, Chengcheng,Sun, Zuodeng,Ji, Chenglong,et al. Toxicological effects of tris(1,3-dichloro-2-propyl) phosphate in oyster Crassostrea gigas using proteomic and phosphoproteomic analyses[J]. JOURNAL OF HAZARDOUS MATERIALS,2022,434:12.
APA Yin, Chengcheng,Sun, Zuodeng,Ji, Chenglong,Li, Fei,&Wu, Huifeng.(2022).Toxicological effects of tris(1,3-dichloro-2-propyl) phosphate in oyster Crassostrea gigas using proteomic and phosphoproteomic analyses.JOURNAL OF HAZARDOUS MATERIALS,434,12.
MLA Yin, Chengcheng,et al."Toxicological effects of tris(1,3-dichloro-2-propyl) phosphate in oyster Crassostrea gigas using proteomic and phosphoproteomic analyses".JOURNAL OF HAZARDOUS MATERIALS 434(2022):12.
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