| A SERS-based competitive immunoassay for highly sensitive and specific detection of ochratoxin A | |
Ding, Yangjun1; Shang, Hezhen2; Wang, Xiaokun3; Chen, Lingxin4,5
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| 发表期刊 | ANALYST
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| ISSN | 0003-2654 |
| 2020-09-21 | |
| 卷号 | 145期号:18页码:6079-6084 |
| 关键词 | COLORIMETRIC APTASENSOR GOLD NANOPARTICLES TOXICITY PROBES RISK |
| DOI | 10.1039/d0an01220c |
| 英文摘要 | Ochratoxin A (OTA) is a well-known carcinogenic contaminant in food commodities. The highly sensitive and specific detection of the OTA level is an essential way to prevent the health risks to humans/animals. In this work, an SERS-based competitive immunoassay platform has been developed for the detection of OTA. This assay contains two compartments: OTA-BSA-immobilized SERS nanotags and anti-OTA antibody-functionalized magnetic beads. In the presence of target OTA, a competitive reaction towards magnetic beads occurs between the target OTA and SERS nanotags. The characteristic peaks of SERS nanotags adsorbed onto magnetic beads were used for the quantitative analysis of OTA. Under optimized conditions, a good linear relationship was obtained in the range of 1 pg mL(-1)to1000 pg mL(-1). The limit of detection (LOD) was estimated to be 0.61 pg mL(-1)using the IUPAC standard method. In addition, this proposed method was also successfully applied for the detection of OTA in wine samples and satisfactory recovery rates were obtained in the range of 90.60% to 103.3%. It is believed that this SERS-based competitive method holds great potential for highly sensitive and specific detection of mycotoxins. |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21904078, 21976209] ; Taishan Scholar Project Special Funding [ts20190962] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | COLORIMETRIC APTASENSOR ; GOLD ; NANOPARTICLES ; TOXICITY ; PROBES ; RISK |
| 研究领域[WOS] | Chemistry |
| WOS记录号 | WOS:000568961600009 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/30492 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心 |
| 作者单位 | 1.Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Shandong, Peoples R China; 2.Qingdao Chengyang Dist Peoples Hosp, Dept Hepatobiliary Surg, Qingdao 266109, Peoples R China; 3.Qingdao Univ Sci & Technol, Coll Marine Sci & Biol Engn, Qingdao 266042, Peoples R China; 4.Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; 5.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ding, Yangjun,Shang, Hezhen,Wang, Xiaokun,et al. A SERS-based competitive immunoassay for highly sensitive and specific detection of ochratoxin A[J]. ANALYST,2020,145(18):6079-6084. |
| APA | Ding, Yangjun,Shang, Hezhen,Wang, Xiaokun,&Chen, Lingxin.(2020).A SERS-based competitive immunoassay for highly sensitive and specific detection of ochratoxin A.ANALYST,145(18),6079-6084. |
| MLA | Ding, Yangjun,et al."A SERS-based competitive immunoassay for highly sensitive and specific detection of ochratoxin A".ANALYST 145.18(2020):6079-6084. |
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