Visualizing and evaluating mitochondrial cysteine via near-infrared fluorescence imaging in cells, tissues and in vivo under hypoxia/ reperfusion stress
Zhang, Xia1,5; Zhang, Liangwei1,3; Wang, Xiaoyan1,4; Han, Xiaoyue1,5; Huang, Yan1,4; Li, Bowei1,3; Chen, Lingxin1,2,3
Source PublicationJOURNAL OF HAZARDOUS MATERIALS
ISSN0304-3894
2021-10-05
Volume419Pages:10
KeywordFluorescent probe Cysteine Near-infrared imaging Hypoxia reperfusion Environmental stress
DOI10.1016/j.jhazmat.2021.126476
Corresponding AuthorChen, Lingxin(lxchen@yic.ac.cn)
AbstractIncreasingly grim environmental pollutions are closely related with the occurrence and development of diseases. However, it's obscure how environmental stress disturbs the normal physiological process, and then how endogenous reactive species mend the cases. Hypoxia/reperfusion (H/R), a common and intractable injury in aquaculture and clinic, can induce oxidative stress and ultimately cause irreversible injury to organism. Cysteine (Cys) plays essential roles in maintaining transduction of numerous reactive species and redox homeostasis in subcellular structures, cells and organisms. A great deal of fluorescence research about Cys are focusing on development of selective probes but with poor exploration of the biofunction under environmental stress. Therefore, it is of great significance to examine the bio-effects of Cys against H/R stress. In the present work, we design a fluorescent probe BCy-AC for in situ detecting Cys, the unique Enol-Keto tautomerization of fluorophore BCy-Keto propels the reaction process which will improve the sensitivity and potential application performance of the probe. BCy-AC is conveniently applied to visualize Cys in HT-22 cells, zebrafish and mice tissues. Moreover, imaging results obtained from H/R models reveal that endogenous Cys changes with hypoxia and reperfusion time and Cys pretreatment effectively defend H/R injury in cells and in vivo.
Funding OrganizationNational Natural Science Foundation of China ; Science and Technology Innovation Development Plan of Yantai of China ; Shandong Provincial Natural Science Founda-tion Key Project ; Program of Youth Innovation Promotion Association, CAS ; Taishan Scholar Project Special Funding
Indexed BySCI
Language英语
WOS KeywordSELECTIVE DETECTION ; GLUTATHIONE DEPLETION ; GOLD NANOPARTICLES ; REACTIVE OXYGEN ; RECENT PROGRESS ; PROBE ; POLYSULFIDES ; LUMINESCENT
WOS Research AreaEngineering ; Environmental Sciences & Ecology
WOS IDWOS:000694784800001
Citation statistics
Cited Times:20[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/29744
Collection中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室
中国科学院海岸带环境过程与生态修复重点实验室
Corresponding AuthorChen, Lingxin
Affiliation1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Key Lab Coastal Environm Proc, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
2.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China
4.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Xia,Zhang, Liangwei,Wang, Xiaoyan,et al. Visualizing and evaluating mitochondrial cysteine via near-infrared fluorescence imaging in cells, tissues and in vivo under hypoxia/ reperfusion stress[J]. JOURNAL OF HAZARDOUS MATERIALS,2021,419:10.
APA Zhang, Xia.,Zhang, Liangwei.,Wang, Xiaoyan.,Han, Xiaoyue.,Huang, Yan.,...&Chen, Lingxin.(2021).Visualizing and evaluating mitochondrial cysteine via near-infrared fluorescence imaging in cells, tissues and in vivo under hypoxia/ reperfusion stress.JOURNAL OF HAZARDOUS MATERIALS,419,10.
MLA Zhang, Xia,et al."Visualizing and evaluating mitochondrial cysteine via near-infrared fluorescence imaging in cells, tissues and in vivo under hypoxia/ reperfusion stress".JOURNAL OF HAZARDOUS MATERIALS 419(2021):10.
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