| Determination of chitosan content with ratio coefficient method and HPLC | |
Miao, Qin1,2; Cui, Yulin1,2 ; Zhang, Jingjing1,2; Mi, Yingqi1,2,3; Tan, Wenqiang1,2; Li, Qing1,2; Gu, Guodong4; Dong, Fang1,2; Guo, Zhanyong1,2,3
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| 发表期刊 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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| ISSN | 0141-8130 |
| 2020-12-01 | |
| 卷号 | 164页码:384-388 |
| 关键词 | Ratio coefficient Determination of chitosan content Hydrolysis rate HPLC Average deacetylation degree (DD) |
| DOI | 10.1016/j.ijbiomac.2020.07.013 |
| 通讯作者 | Guo, Zhanyong(zhanyongguo@hotmail.com) |
| 英文摘要 | The determination of chitosan is a significant topic that has been growing in importance for a variety of fields. To quantify chitosan, various analytical methods can be used. When high performance liquid chromatographic (HPLC) is used for quantitative analysis of chitosan, the chitosan is mainly hydrolyzed into glucosamine salts, which are determined by HPLC. Average deacetylation degree (DD) has an effect on acid hydrolysis of chitosan. In this paper, chitosan with different average DDs was hydrolyzed to observe its effect on the determination of chitosan content, and that the study of combining acid hydrolysis rates of chitosan (alpha) with different average DDs and chitin (beta) standard to form ratio coefficients (gamma) to quantify chitosan was designed. Then, the table about. values within certain average DD ranges of chitosan was obtained. By using. and a, the actual content of chitosan could be obtained simply and accurately. (C) 2020 Elsevier B.V. All rights reserved. |
| 资助机构 | National Key Research and Development Programof China ; Key Research and Development Program of Shandong Province ; Natural Science Foundation of Shandong Province of China ; Youth Innovation Promotion Association CAS |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | ACID-HYDROLYSIS ; LIQUID-CHROMATOGRAPHY ; DEACETYLATION ; GLUCOSAMINE ; CHITIN ; ACETYLATION |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| WOS记录号 | WOS:000588093700035 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/27523 |
| 专题 | 海岸带生物学与生物资源利用重点实验室 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室 海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心 |
| 通讯作者 | Guo, Zhanyong |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai 264003, Shandong, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Alliance Pharma Inc, 17 Lee Blvd, Malvern, PA 19355 USA |
| 推荐引用方式 GB/T 7714 | Miao, Qin,Cui, Yulin,Zhang, Jingjing,et al. Determination of chitosan content with ratio coefficient method and HPLC[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2020,164:384-388. |
| APA | Miao, Qin.,Cui, Yulin.,Zhang, Jingjing.,Mi, Yingqi.,Tan, Wenqiang.,...&Guo, Zhanyong.(2020).Determination of chitosan content with ratio coefficient method and HPLC.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,164,384-388. |
| MLA | Miao, Qin,et al."Determination of chitosan content with ratio coefficient method and HPLC".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 164(2020):384-388. |
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