Glucose biosensor using fluorescence quenching with chitosan-modified graphene oxide

doi:10.1049/mnl.2018.5269

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	Glucose biosensor using fluorescence quenching with chitosan-modified graphene oxide
	Li, WJ ; Jiang, TT ; Pu, Y ; Jiao, XD; Tan, WQ; Qin, S
发表期刊	MICRO & NANO LETTERS
ISSN	1750-0443
	2019-03-06
卷号	14 期号:3 页码:344-348
关键词	biochemistry biosensors nanosensors nanofabrication fluorescence molecular biophysics radiation quenching chemical sensors sugar proteins biomedical optical imaging graphene compounds GO-CS low concentrations competitive binding maltose-binding protein recombinant phycocyanin far-red fluorescence MBP-rPC rPC emission rPC fluorescence linear detection range glucose biosensor fluorescence quenching chitosan-modified graphene oxide low-molecular-weight chitosan glucose detection fluorescence resonance energy transfer graphene oxide nanomaterial mass 0 1 mg to 1 0 mg CO
研究领域	Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
DOI	10.1049/mnl.2018.5269
产权排序	[Li, Wenjun; Jiao, Xudong; Tan, Wenqiang; Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Jiang, Tingting] Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China; [Pu, Yang] Ludong Univ, Sch Agr, Yantai 264025, Peoples R China
作者部门	海岸带生物学与生物资源保护实验室
英文摘要	A novel graphene oxide (GO) nanomaterial, which was rapidly and simply synthesised and conjugated with low-molecular-weight chitosan (CS), was used to develop a simple, sensitive GO biosensor system for glucose detection by fluorescence resonance energy transfer. The GO-CS was used to detect low concentrations of glucose based on competitive binding with maltose-binding protein (MBP). The -subunit of recombinant phycocyanin (rPC), which emits far-red fluorescence, was used to label MBP (MBP-rPC). The rPC emission was quenched by binding between GO-CS and MBP. However, in the presence of glucose, GO-CS was out-competed for binding to MBP, leading to rPC fluorescence. Glucose was sensitively and selectively detected with this biosensor, with a linear detection range of 0.1-1 mg glucose/ml. The limit of detection for glucose was approximate to 0.05 mg/ml.
文章类型	Article
资助机构	Marine Economy Innovation Development Demonstration Project State Oceanic Administration of China [YHCX-SW-P-201701, YHCX-SW-Y-201701] ; Scientific Research Foundation of Ludong University [ly2014041]
收录类别	SCI
语种	英语
关键词[WOS]	FRET BIOSENSOR ; PURIFICATION ; MANAGEMENT ; PROTEIN ; SENSOR ; DNA
研究领域[WOS]	Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS记录号	WOS:000461572800025
引用统计	被引频次：10[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/24977
专题	海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; 2.Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China; 3.Ludong Univ, Sch Agr, Yantai 264025, Peoples R China
推荐引用方式 GB/T 7714	Li, WJ,Jiang, TT,Pu, Y,et al. Glucose biosensor using fluorescence quenching with chitosan-modified graphene oxide[J]. MICRO & NANO LETTERS,2019,14(3):344-348.
APA	Li, WJ,Jiang, TT,Pu, Y,Jiao, XD,Tan, WQ,&Qin, S.(2019).Glucose biosensor using fluorescence quenching with chitosan-modified graphene oxide.MICRO & NANO LETTERS,14(3),344-348.
MLA	Li, WJ,et al."Glucose biosensor using fluorescence quenching with chitosan-modified graphene oxide".MICRO & NANO LETTERS 14.3(2019):344-348.

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