Glucose biosensor using fluorescence quenching with chitosan-modified graphene oxide | |
Li, WJ; Jiang, TT; Pu, Y; Jiao, XD![]() ![]() ![]() | |
发表期刊 | MICRO & NANO LETTERS
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ISSN | 1750-0443 |
2019-03-06 | |
卷号 | 14期号:3页码:344-348 |
关键词 | biochemistry biosensors nanosensors nanofabrication fluorescence molecular biophysics radiation quenching chemical sensors sugar proteins biomedical optical imaging graphene compounds GO-CS low concentrations competitive binding maltose-binding protein recombinant phycocyanin far-red fluorescence MBP-rPC rPC emission rPC fluorescence linear detection range glucose biosensor fluorescence quenching chitosan-modified graphene oxide low-molecular-weight chitosan glucose detection fluorescence resonance energy transfer graphene oxide nanomaterial mass 0 1 mg to 1 0 mg CO |
研究领域 | Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary |
DOI | 10.1049/mnl.2018.5269 |
产权排序 | [Li, Wenjun; Jiao, Xudong; Tan, Wenqiang; Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Jiang, Tingting] Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China; [Pu, Yang] Ludong Univ, Sch Agr, Yantai 264025, Peoples R China |
作者部门 | 海岸带生物学与生物资源保护实验室 |
英文摘要 | A novel graphene oxide (GO) nanomaterial, which was rapidly and simply synthesised and conjugated with low-molecular-weight chitosan (CS), was used to develop a simple, sensitive GO biosensor system for glucose detection by fluorescence resonance energy transfer. The GO-CS was used to detect low concentrations of glucose based on competitive binding with maltose-binding protein (MBP). The -subunit of recombinant phycocyanin (rPC), which emits far-red fluorescence, was used to label MBP (MBP-rPC). The rPC emission was quenched by binding between GO-CS and MBP. However, in the presence of glucose, GO-CS was out-competed for binding to MBP, leading to rPC fluorescence. Glucose was sensitively and selectively detected with this biosensor, with a linear detection range of 0.1-1 mg glucose/ml. The limit of detection for glucose was approximate to 0.05 mg/ml. |
文章类型 | Article |
资助机构 | Marine Economy Innovation Development Demonstration Project State Oceanic Administration of China [YHCX-SW-P-201701, YHCX-SW-Y-201701] ; Scientific Research Foundation of Ludong University [ly2014041] |
收录类别 | SCI |
语种 | 英语 |
关键词[WOS] | FRET BIOSENSOR ; PURIFICATION ; MANAGEMENT ; PROTEIN ; SENSOR ; DNA |
研究领域[WOS] | Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary |
WOS记录号 | WOS:000461572800025 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.yic.ac.cn/handle/133337/24977 |
专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心 |
作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; 2.Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China; 3.Ludong Univ, Sch Agr, Yantai 264025, Peoples R China |
推荐引用方式 GB/T 7714 | Li, WJ,Jiang, TT,Pu, Y,et al. Glucose biosensor using fluorescence quenching with chitosan-modified graphene oxide[J]. MICRO & NANO LETTERS,2019,14(3):344-348. |
APA | Li, WJ,Jiang, TT,Pu, Y,Jiao, XD,Tan, WQ,&Qin, S.(2019).Glucose biosensor using fluorescence quenching with chitosan-modified graphene oxide.MICRO & NANO LETTERS,14(3),344-348. |
MLA | Li, WJ,et al."Glucose biosensor using fluorescence quenching with chitosan-modified graphene oxide".MICRO & NANO LETTERS 14.3(2019):344-348. |
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